Life Sciences Commons^™

Differences In Lean And Obese Skeletal Muscle Myoblasts Of Adult And Post-Natal La/N (Faf) Rats: A Confocal Description Of The Skeletal Muscle Myoblast, J. Adam Noah

Theses, Dissertations and Capstones

Mutant strains of obese rats are currently being studied to reveal the mechanisms of increased lipid synthesis and decreased lipid oxidation in these rats. It was originally thought that much of the fat production that leads to obesity is the result of metabolic processes in the liver and adipose tissue. Studies by Kahle et al.³ showed that the liver and adipose tissue account for only 22% and 7% respectively, of total fatty acid synthesis. It was also shown that 21 % of total de novo fatty acid was localized in the postural skeletal muscle.

The dry weight of the …

Ecology And Natural History Of The Cow Knob Salamander, Plethodon Punctatus Highton, In West Virginia, Robert B. Tucker

Theses, Dissertations and Capstones

Very little has been written on the ecology and natural history of the Cow Knob salamander, Plethodon punctatus Highton, since it was described by Highton (1971). Plethodon punctatus is known only from the higher elevations of Great North (above 2,800 ft) and Shenandoah (above 3,000 ft.) Mountains in Virginia and West Virginia (Highton, 1972; Conant and Collins, 1991). The type locality is Cow Knob, Pendleton County, West Virginia. Plethodon punctatus is a large (up to 75 mm snout-to-vent length) dorso-ventrally flattened salamander with creamish iridiophore spots on the back and sides, webbed feet for a fossorial lifestyle, and large …

The Use Of Isoelectric Focusing To Assess The Genetic Diversity Of The Cyprinid Fishes: Rhinichthys Cataractae And Rhinichthys Atratulus In West Virginia, Fauzia Alam Miah

Theses, Dissertations and Capstones

In this study, isoelectric focusing is used to separate proteins of Rhinichthys cataractae and R. atratulus and conventional staining is used to identify alleles of six genes. The objective of this work is to determine whether these methods can effectively assay isozymes that may be used to establish the relationship between anthropogenic stress and genetic diversity. Alleles for LDH-A, LDH-B, EST-A, EST-B, EST-C, and EST-D loci were identified for two species of fish, R. atratulus and R. cataractae. Both fish species are known to be sensitive to anthropogenic stress and known to be widely distributed in stressed environments. Alleles …

Influences Of Acid Mine Drainage On Water Chemistry Of Blackwater River Watershed, Carla C. Morato-Lara

Theses, Dissertations and Capstones

Acidic mine drainage flow from active and abandoned coal mines has been a major source of water pollution in the Appalachian Mountain region of the eastern USA. Water samples were collected and analyzed from Blackwater River watershed during 2 years (September 1996 - May 1998). The water quality measurements determined that the Beaver Creek and North Fork tributaries were affected by acid mine drainage but not Yellow Creek. Beaver Creek and North Fork stations had high sulfate and manganese markers of acid mine drainage, whereas Yellow Creek was slightly acidic with low sulfate and manganese. North Fork was the primary …

Ecological Life History Of Caenis Amica Hagen (Emphemeroptera: Caenidae) From The Mitigated Area Of Green Bottom Wildlife Management Area, Cabell County, West Virginia, Gail Lynn Perrine

Theses, Dissertations and Capstones

The ecological life history of a population of Caenis amica Hagen from the Green Bottom Wildlife Management Area was studied from November 1996 to November 1997. Water quality data and 2,505 larvae were collected from the newly mitigated area of Green Bottom Swamp. Total body lengths of larvae were measured using a computer-digitizing program. These results indicated a bivoltine life cycle. Most larvae were found in the spring and summer months of April, June, and July. The highest peak of larvae (1,035) occurred in July. The pH values ranged from 7.0 to 8.0 with a mean of 7.6. Temperatures ranged …

Life History, Ecology, And Low Ph Tolerance Of The Freshwater Prawn, Palaemonetes Kadiakensis Rathbun, In A Mitigated Wetland Of The Green Bottom Wildlife Management Area, West Virginia, Jennifer Ann Aderman

Theses, Dissertations and Capstones

An ecological life history study of the freshwater prawn Palaemonetes kadiakensis was conducted at the mitigated wetland of the Green Bottom Wildlife management Area (GBWMA). Monthly samples were collected from November 1996 through November 1997. In addition, weekly samples were collected from May through November for growth and reproductive analysis. Monthly samples yielded 520 prawns. Palaemonetes kadiakensis had a one-year life cycle, with all adults dying by September. Males averaged 23.82 mm (range = 14.15 to 36.75 mm) and females averaged 26.78mm (range = 15.06 to 38.60 mm) in length. Males averaged 0.087g (range = 0.014to 0.293 g) and females …

An Assessment Of The Prevalence Of The Bass Tapeworm, Proteocephalus Ambloplitis, And Other Parasites In Five Species Of Basses From River And Lake Ecosystems In West Virginia, J. Alyce Bradbury

Theses, Dissertations and Capstones

Two-hundred and fifteen basses were collected from four river sites (Racine Locks and Dam, Robert C. Byrd Locks and Dam; Ashland, KY—Ohio River; London Locks and Dam—Kanawha River) and five reservoir sites (Beech Fork Lake, East Lynn Lake, Summersville Lake, Burnsville Lake, and Stonewall Jackson Lake,). The five species of bass caught were: largemouth bass, Micropterus salmoides; smallmouth bass, Micropterus dolomieui; spotted bass, Micropterus punctulatus; white bass, Morone chrysops; and hybrid bass, Morone chyrsops x Morone saxatilis. Basses were collected by three methods: 1) local anglers (hook and line), 2) gill netting surveys; and 3) …

The Mechanics And Regulation Of Rat Aortic Smooth Muscle Contraction: Implications Of Cytoskeletal Remodeling, Protein Phosphorylations, And Microtubule-Based Kinase Transport, Alison Summers Battistella-Patterson

Theses, Dissertations and Capstones

The exact nature of the mechanisms and the regulation of vascular smooth muscle contraction is not well understood. To better understand these processes, we examined two systems involved in smooth muscle contraction, the cytoskeleton and the protein kinases. In order to study the role of the cytoskeleton in smooth muscle contraction, we examined the contractile and mechanical effects of cytoskeleton disruption. We found that the relationship between passive tension applied to aortic rings and the resulting increase in tissue length was nearly linear over the range of 1 g to 15 g. However, even with increasing tissue length, within the …

The Role Of Domain I In Laminin Chain Assembly, George D. Kamphaus

Theses, Dissertations and Capstones

Laminin, a major component of basement membrane, is a trimeric glycoprotein comprised of three chains - α, β and γ (Burgeson et al., 1994). An order for trimer assembly has been deduced: first, the β and γ chains bind to form a dimer and subsequently α is added to complete the trimer (I. Hunter et al., 1990 & 1992; Utani et al., 1994 & 1995). The C-terminal portions, found within the protein structural domain I of the p and y chains, are implicated in dimer and trimer formation by biochemical studies performed extracellularly (Utani, et al., 1994 & 1995; Nomizu …

Molecular Mapping Of The Fatty (F) (Formerly Corpulent) Gene In The La/N Rat Obesity Model, Kenneth G. Butz

Theses, Dissertations and Capstones

The LA/N-corpulent (cp) rat obesity model was a congenic rat strain developed at the National Institutes of Health by C. T. Hansen . The LA/N-cp rat originated from breeding the Koletsky rat, possessing the corpulent gene, with the normotensive LA/N inbred rat. The autosomal recessive single gene mutation, cp, produces obesity with hyperphagia, hyperinsulinemia and elevated activities of liver and skeletal muscle lipogenic enzymes in the LA/N-cp rat model. The Zucker-fatty mutation, which was mapped to rat chromosome , resulted in a comparable, but not identical obesity syndrome. Breeding experiments by Yen et al …

The Use Of Emergent Rocks As Refugia For The Cheat Mountain Salamander, Plethodon Nettingi Green, Beth Anne Pauley

Theses, Dissertations and Capstones

Plethodon nettinqi was listed as a threatened species in 1989 by the United States Fish and Wildlife Service (Pauley, 1991). Its total range is within 5 counties in eastern West Virginia. There are fewer then 60 disjunct populations known and most populations are above 3,000 ft. and are associated with emergent rocks or narrow ravines with Rhododendron. It is hypothesized the P. netting! survived lumbering practices at the turn of the century by taking refuge beneath large emergent rocks and narrow ravines with Rhododendron. This study examined environmental factors associated with emergent rocks that might regulate the distribution of P. …