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The Pax-5 Gene Is Alternatively Spliced During B-Cell Development, Patty Zwollo, Hector Arrieta, Kaleo Ede, Karen Molinder, Stephen Desiderio, Roberta Pollock
The Pax-5 Gene Is Alternatively Spliced During B-Cell Development, Patty Zwollo, Hector Arrieta, Kaleo Ede, Karen Molinder, Stephen Desiderio, Roberta Pollock
Roberta Pollock
The transcription factor Pax-5 is expressed during the early stages of B-cell differentiation and influences the expression of several B-cell-specific genes. In addition to the existing isoform (Pax-5, which we have named Pax-5a), we have isolated three new isoforms, Pax-5b, Pax-5d, and Pax-5e, from murine spleen and B-lymphoid cell lines using library screenings and polymerase chain reaction amplification. Isoforms Pax-5b and Pax-5e have spliced out their second exon, resulting in proteins with only a partial DNA-binding domain. Isoforms Pax-5d and Pax-5e have deleted the 3′-region, which encodes the transactivating domain, and replaced it with a novel sequence. The existence of …