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Spiroplasma Lineolae Sp. Nov., From The Horsefly Tabanus Lineola (Diptera : Tabanidae), Frank E. French, Robert F. Whitcomb, Joseph G. Tully, Patricia Carle, Joseph M. Bové, Roberta B. Henegar, Jean R. Adams, Gail E. Gasparich, David L. Williamson
Spiroplasma Lineolae Sp. Nov., From The Horsefly Tabanus Lineola (Diptera : Tabanidae), Frank E. French, Robert F. Whitcomb, Joseph G. Tully, Patricia Carle, Joseph M. Bové, Roberta B. Henegar, Jean R. Adams, Gail E. Gasparich, David L. Williamson
Gail Gasparich
Spiroplasma strain TALS-2T from the viscera of the striped horsefly, Tabanus lineola, collected in Georgia was serologically distinct from other Spiroplasma species, groups, putative groups, and subgroups. Light and electron microscopy of cells of strain TALS-2T revealed helical motile cells surrounded only by a single cytoplasmic membrane. The organism grew in M1D and SP-4 liquid media. Growth also occurred in 1% serum fraction medium and in conventional horse serum medium. Growth in liquid media was serum dependent. The strain passed through 220-nm filter pores, but was retained in filters with 100-nm pores. The optimum temperature for growth was 30°C. Multiplication …