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Collaborative Roles For C-Jun N-Terminal Kinase, C-Jun, Serum Response Factor, And Sp1 In Calcium-Regulated Myocardial Gene Expression, Patrick M. Mcdonough, Deanna S. Hanford, Amy B. Sprenkle, Noel R. Mellon, Christopher C. Glembotski
Collaborative Roles For C-Jun N-Terminal Kinase, C-Jun, Serum Response Factor, And Sp1 In Calcium-Regulated Myocardial Gene Expression, Patrick M. Mcdonough, Deanna S. Hanford, Amy B. Sprenkle, Noel R. Mellon, Christopher C. Glembotski
Amy Sprenkle
Electrical stimulation of contractions (pacing) of primary neonatal rat ventricular myocytes increases intracellular calcium and activates a hypertrophic growth program that includes expression of the cardiac-specific gene, atrial natriuretic factor (ANF). To investigate the mechanism whereby pacing increases ANF, pacing was tested for its ability to regulate mitogen-activated protein kinase family members, ANF promoter activity, and the trans-activation domain of the transcription factor, Sp1. Pacing and the calcium channel agonist BAYK 8644 activated c-Jun N-terminal kinase (JNK) but not extracellular signal-regulated kinase. Pacing stimulated ANF-promoter activity approximately 10-fold. Furthermore, transfection with an expression vector for c-Jun, a substrate for JNK, …