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Full-Text Articles in Life Sciences
The Role Of Calcium In The Regulation Of Apoptosis, David J. Mcconkey
The Role Of Calcium In The Regulation Of Apoptosis, David J. Mcconkey
Scanning Microscopy
The recognition that apoptosis is regulated by an evolutionarily conserved set of polypeptides from the nematode Caenorhabditis elegans to humans suggests that a conserved set of biochemical mechanism(s) may also be involved in the response. Early evidence suggested that the endogenous endonuclease implicated in apoptosis in most model systems is Ca2+ -dependent, and subsequent work from a number of independent laboratories suggests that alterations in cytosolic Ca2+ homeostasis are one of the conserved biochemical pathways regulating the response. Molecular targets for Ca2+ are now being identified and include signal transduction intermediates, endonuclease(s) and proteases, and the enzymes involved in the …
Applications Of Laser Microprobe Mass Spectrometry In Biology And Medicine, Annette Bakker, Luc Van Vaeck, Wim Jacob
Applications Of Laser Microprobe Mass Spectrometry In Biology And Medicine, Annette Bakker, Luc Van Vaeck, Wim Jacob
Scanning Microscopy
Laser microprobe mass spectrometry (LMMS) provides spot analysis with a lateral resolution of 1-5 μm. Focused laser ionisation, combined with mass spectrometry, yields information on elements, molecular identification of inorganic substances, and structural characterisation of organic molecules of typically less than 2-3 kDa. Quantification in complex heterogeneous systems, such as biological materials, is not possible at the present state of the art. The strength of the method lies in the qualitative information on the molecular composition of the analyte, not just element detection, with a lateral resolution at the light microscopy level. The applications of this technique in the field …