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Full-Text Articles in Life Sciences
Detection Of Haplosporidium-Nelsoni (Haplosporidia, Haplosporidiidae) In Oysters By Pcr Amplification, Na Stokes, Me Siddall, Em Burreson
Detection Of Haplosporidium-Nelsoni (Haplosporidia, Haplosporidiidae) In Oysters By Pcr Amplification, Na Stokes, Me Siddall, Em Burreson
VIMS Articles
Haplosporidium nelsoni is a protistan pathogen of the eastern oyster Crassostrea virginica, and has contributed to the decline of the oyster population in the Chesapeake Bay. From comparison of the sequence data of the 16S-like rDNA of H. nelsoni with those of Minchinia teredinis and other related organisms, 2 oligonucleotides which were specific to H. nelsoni and suitable for use as PCR primers were identified. These primers amplified a 564 base pair fragment of the small subunit (SSU) rRNA gene of H. nelsoni, but did not amplify genomic oyster DNA or the SSU rRNA genes of the haplosporidians Haplosporidium costale, …
Heat-Shock Proteins Of The Oyster Parasite Perkinsus-Marinus, Ct Tirard, Rm Grossfeld, Ak Volety, Fle Chu
Heat-Shock Proteins Of The Oyster Parasite Perkinsus-Marinus, Ct Tirard, Rm Grossfeld, Ak Volety, Fle Chu
VIMS Articles
The susceptibility of the eastern oyster Crassostrea virginica to infection by the protozoan parasite Perkinsus marinus is influenced by temperature. Because of the crucial roles of heat shock proteins in cellular thermal tolerance and in host-parasite adaptations in other species, we compared the in vitro heat shock responses of cultured P. marin us and of oyster hemocytes. The parasite and host heat shock proteins were different in size and in immunochemical specificity. In addition, the thermal threshold for inducing the response was higher for P. marinus acclimated to the same temperature as the oysters. The results suggest that EI marinus …