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Liver Lipids Profiles In Nude Mice Implanted Subcutaneously With Cells Of Human Prostate Adenocarcinoma Grade Iv, Lawrence M. Mwasi
Liver Lipids Profiles In Nude Mice Implanted Subcutaneously With Cells Of Human Prostate Adenocarcinoma Grade Iv, Lawrence M. Mwasi
Journal of the Arkansas Academy of Science
Liver lipid changes in male BALB-c nude mice due to subcutaneously implanted human prostate metastatic grade IV adenocarcinoma was studied. The prostate cancer cells were cultured in F12 plus 7.5% horse serum and 25% fetal calf serum medium. When they reached confluence, some of these cells were fixed with glutaraldehyde and thoroughly washed with buffer then 4 x107 cells were implanted into four mice. Four more mice were implanted with 4 x 106 viable, unfixed cells. Four uninjected mice served as controls. All the mice were sacrificed 18 days later. The total liver lipids (TLL) from each liver were extracted …