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The Phosphate Transporter From Pea Mitochondria (Isolation And Characterization In Proteolipid Vesicles), Cecilia A. Mcintosh, David J. Oliver
The Phosphate Transporter From Pea Mitochondria (Isolation And Characterization In Proteolipid Vesicles), Cecilia A. Mcintosh, David J. Oliver
David J. Oliver
The phosphate transporter from mitochondria will exchange matrix phosphate for cytosolic phosphate and facilitate either phosphate/proton symport or phosphate/hydroxyl ion antiport. The phosphate transported into the matrix by this carrier is either used for ATP synthesis or exchanges back out to the cytosol on the dicarboxylate transporter, permitting entry of malate and succinate into the matrix. The phosphate transporter was solubilized from etiolated pea (Pisum sativum L. cv Alaska) mitochondrial membranes with Triton X-114, purified approximately 500-fold by hydroxylapatite chromatography, and reconstituted into azolectin vesicles that were preloaded with 0.1 or 10 mM phosphate. Phosphate transport was measured as the …