Life Sciences Commons^™

The Effect Of Viscosity In Beveled Blade Coating, Salih Cebe

Masters Theses

The objective of this study was to investigate the effects of several types of coating viscosity on coat weight development during the beveled blade coating process. In this study, the effects of several types of coating viscosity were experimentally investigated at the applicator roll, before and underneath the blade. Coating color viscosities were changed through two methods. The first method involved changing the temperature of coating color at constant carboxymethylcellulose (CMC) concentration, while the second method consisted of varying the CMC concentration in coating formula at constant coating temperature.

Coating viscosity had an influencing effect both on the amount of …

A Socio-Economic Study Of Nature Tourist Behavior In Louisiana, Rudolf Walter Jacobs Ast

Honors Theses

No abstract provided.

The Slip Velocity Of Coating Formulations Using The High-Shear Viscometer, Daniel Parker Krieger

Masters Theses

When using a cup and bob viscometer, it has long been recognized that errors in the viscosity measurement can arise from several factors, among them slip. Slip is a phenomenon which occurs when there are slipping layers in the fluid under shear, causing a non-uniform velocity gradient to be generated across the gap of the cup and bob viscometer. A layer of fluid at either the bob or cup wall moves faster than the adjoining layers, causing the slipping effect. This leads to a low viscosity calculation for the fluid.

In this study, experiments with coating formulations were conducted using …

Nitric Oxide Production: A Mechanism For Inhibition Of Chlamydia Trachomatis Replication, Bojun Chen

Electronic Theses and Dissertations

Chlamydia trachomatis (CT) replicates in macrophages, but is inhibited by IFN-$\gamma$ or LPS. IFN-$\gamma$ and/or LPS induced nitrite production in mouse peritoneal macrophages, macrophage cell lines (RAW264.7 and J774A.1) and McCoy cells. Kinetic studies indicated that peak production occurred 48 hours post-treatment. CT infection itself was insufficient to induce nitrite production, but resulted in enhancement of nitrite production in IFN-$\gamma$-treated cells. Treatment with IFN-$\gamma$ or LPS resulted in significant inhibition of CT replication in these cells. Strong correlation between nitrite production and inhibition of CT replication was observed in RAW264.7 and J774A.1 cells (correlation coefficients: $-$0.93 and $-$0.94, p $<$ 0.001). N$\sp{\rm g}$- monomethyl-L-arginine (L-NMMA) specifically inhibited nitrite production and partially reversed inhibition of CT replication in macrophage cell lines. NOS mRNA was measured in RAW264.7 cells by Northern blot and Dot blot hybridization. Strong correlation between NOS mRNA expression and inhibition of CT replication (correlation coefficient: $-$0.97, p $<$ 0.05) was observed. Anti-TNF-$\alpha$ antibody completely neutralized the biological activity of TNF-$\alpha$ secreted by LPS-treated RAW264.7 cells, yet the antibody neither reduced nitrite production nor restored CT replication. Combination of the antibody and L-NMMA significantly enhanced restoration of CT replication. In peritoneal macrophages, inhibition of CT replication induced by IFN-$\gamma$ was partially restored by L-NMMA or anti-TNF-$\alpha$ antibody. In McCoy cells, inhibition of CT replication induced by IFN-$\gamma$ and LPS was not significantly restored by L-NMMA. Great restoration of CT replication by 1 mM L-NMMA was observed in LPS-treated J774A.1 cells (31%), but not in IFN-$\gamma$-treated cells (5%). Our data indicate that (1) NO production is one of the mechanisms for inhibition of CT replication in IFN-$\gamma$-activated peritoneal macrophages and RAW264.7 cells; (2) NO plays a significant role in CT inhibition in LPS-treated macrophage cell lines, but not peritoneal macrophages; (3) TNF-$\alpha$ may be associated with inhibition, but the mechanism(s) may not involve NO production; (4) NO production may not be the mechanism for CT inhibition in McCoy cells treated with IFN-$\gamma$ and LPS.

A Molecular Basis For Erythromycin Sensitivity And Resistance In Escherichia Coli, Harold S. Chittum

Electronic Theses and Dissertations

The effect of erythromycin on the 50S ribosomal subunit during cell growth has been extensively investigated. Sucrose density gradient analysis of ribosomes formed in the presence and absence of the drug revealed a 50S specific assembly defect is partially responsible for erythromycin's inhibitory effects on wild type cells. Examination of two erythromycin-resistant mutants of E. coli (N281 and N282) revealed that mutant N281 (L22 mutant) but not N282 (L4 mutant) was assembly defective in the presence of the drug, although only at much higher drug concentrations (300 ug/ml vs. 75 ug/ml for wild type cells). The altered genes from each …

A Characterization Of Extractable, Hydroxylated Fatty Acid Bearing Components In Legionella Pneumophila, Jonathan R. Lane

Electronic Theses and Dissertations

Extraction of the lipids of Legionella pneumophila yields phases unlike those produced from other Gram-negative bacteria. A viscous interface forms between the aqueous (wash) and organic phases. More than half of the hydroxylated fatty acids were found distributed between the aqueous phase and the interfacial material, fractions in which such constituents have not been reported in other Gram-negative species. It was further observed that after the material from the aqueous/interfacial phase was dissolved in methanol or chloroform/methanol (2:1 (V/V)), the addition of acetone would create a white, flocculent precipitate. Analyses showed that the supernatant contained fatty acids that were nonhydroxylated …

The Effect Of Poly-Aluminum Silicate Sulfate Retention Of Colored Papers, Tami Frederick

Paper Engineering Senior Theses

The purpose of this thesis experiment was to study the effect of Poly-Aluminum Silicate Sulfate (PASS) on the retention of colored papers. This was performed to reduce the usage of Melamine Formaldehyde based retention aids due to their negative environmental impacts as well as to reduce the usage of Sodium Aluminate, a cationic additive and pH buffer, due to it's negative fade impact on colored papers.

This was accomplished using PASS as the retention aid mechanism and caustic soda as well as soda ash as pH buffers and cationic additives. Paper was made under alkaline conditions. Colored pigments were utilized …

Perceptions Of Current Club Managers Regarding College Coursework Necessary For Preparation For A Career As A Club Manager, Candice E. Clemenz

Masters Theses

The purpose of this research was to gather and interpret the perceptions of current club managers regarding the college coursework that they believed was necessary to prepare a student for a career as a club manager. First, a list of course areas was developed via a review of hospitality curriculums, and conversations with industry professionals. The list was used to design Part 1 of a questionnaire that featured 13 course areas with corresponding descriptive and ascending levels of competencies. Instructions clearly directed club managers to select the highest level of competency for each course area that they thought should be …

Complete Nucleotide Sequence Of Psti-L Fragment Of Tanapox Virus Genomic Dna, Sarah J. Neering

Masters Theses

Tanapox virus (TPV), of the genus Yatapoxvirus, is a human poxvirus that was originally isolated from the Tana River Valley in Kenya in 1962. TPV produces a mild disease characterized by a short febrile illness associated with one or more nodular skin lesions. Although there have been limited studies on TPV, other poxviruses such as vaccinia virus, have been extensively characterized and their genomic DNAs sequenced. In order to examine the molecular features of TPV, we have cloned approximately 17% of the genomic DNA. TPV DNA was digested with Pstl restriction endonuclease and cloned into pUC19. The Pst …

Validation Of A Bag Sampling Method For 1,3-Butadiene, Lori Eckenrode

Community & Environmental Health Theses & Dissertations

At this time there is no NIOSH approved means for bag sampling 1,3-butadiene. An attempt was made to use the application of cold (0°F) to inhibit the reactivity of the gas prior to analysis. The State of California Air Resources Board has an approved method for the gas chromatographic analysis of 1,3-butadiene from stationary sources. The method was utilized in the application of industrial hygiene monitoring of affected workers. The application of cold was found to be ineffective at inhibiting the highly reactive nature of 1,3-butadiene. Analyte losses were too great to classify the method as an accurate measure of …

Effect Of Lead On Globin Mrna In Vivo And In Vitro, C. A. Aethranis

Masters Theses

The total amount of mRNA recovered from Pb²⁺-treated rabbits was not significantly influenced by lead but dose-dependent loss of template activity of the poly A+ fraction was observed. These observations lead to the conclusion that the messenger RNA of rabbit reticulocytes exposed to Pb²⁺ in vivo was damaged and inactivated without extensive depolymerization of the mRNA.

In vitro experiments on the effect of Pb²⁺ on purified rabbit globin mRNA were also performed. After incubation with Pb²⁺ at micromolar concentrations, the 7-methyl guanosine cap was removed from the mRNA, while damage to the remainder of the …

Identification And Evaluation Of Nutrition Education Materials For Use In Kindergarten, Mary Katherine Powers

Masters Theses

The purpose of this project was to determine the extent to which nutrition education lesson plans address the objectives covered in a kindergarten curriculum that is representative of other kindergarten curricula across the United States. Incorporating nutrition education into the kindergarten curriculum can allow children to be exposed to nutrition more often without adding additional subject areas and time to teachers' busy schedules.

We recruited volunteer teachers in a metropolitan school system and provided continuing education on the Dietary Guidelines. Teachers determined that four of the Dietary Guidelines were developmentally appropriate for kindergarten students and evaluated 400 lesson plans as …

Co-Sensitization Of Dopamine And Serotonin Receptors Occurs In The Absence Of A Change In The Dopamine D1 Receptor Complex After A Neonatal 6-Ohda Lesion, Li Gong

Electronic Theses and Dissertations

To test whether SKF 38393 could ontogenetically sensitize dopamine (DA) D$\sb1$ receptors and whether this sensitization would be associated with biochemical changes, intact and neonatal 6-hydroxydopamine (6-OHDA)-lesioned rats (200 $\mu$g i.c.v.) were treated daily from birth with SKF 38393 (3.0 mg/kg i.p. x 28 days) or its vehicle. In DA D$\sb1$ neonatally sensitized 6-OHDA rats, enhanced locomotor responses were observed with the first SKF 38393 challenge dose (3.0 mg/kg i.p.) at 6 weeks. This response increased further with weekly SKF 38393 treatments. Enhanced stereotyped behaviors were seen in both lesioned and sensitized rats at 8 weeks. There was no change …

Tyrosyl Protein Phosphorylation In Human Bone Cells : Its Potential Role In Mediating Fluoride Induced Osteoblast Proliferation, Alan Blair Thomas

Loma Linda University Electronic Theses, Dissertations & Projects

This dissertation was designed to test the hypothesis that fluoride-stimulated osteoblast proliferation is mediated by specific inhibition of a phosphotyrosyl protein phosphatase, resulting in increased steady state levels of tyrosyl phosphorylation of key cellular proteins. Cellular levels of tyrosyl phosphorylation were determined by an immunoblot detection assay in which cellular proteins were separated by SDS-PAGE, transblotted to nitrocellulose membranes and the phosphotyrosyl proteins were identified by anti-phosphotyrosine monoclonal antibodies. The relative amount of tyrosyl phosphorylation was quantitated by laser densitometric analysis of the autoradiograms. This method showed that fluoride induced dose- and time-dependent changes in the level of tyrosyl phosphorylation …

Unusual Structure Of A Human Middle Repetitive Dna, Duminda D. Ratnasinghe

Electronic Theses and Dissertations

The L2Hs sequences are a polymorphic, interspersed, middle repetitive DNA family unique to human genomes. Genomic fingerprinting indicates that these DNAs vary from one individual to another and between tissues of the same individual. Sequence analysis reveals that they are AT-rich (76%) and contain many unusual sequence arrangements (palindromes, inverted and direct repeats). These sequence properties confer on the L2Hs elements the potential to fold into non-B-form structures, a characteristic of recombination hot spots. To test this hypothesis carbodiimide, osmium tetroxide and S$\sb1$ nuclease were used as single-strand specific probes to study a recombinant plasmid, pN6.4.39, containing a single L2Hs …

Characterization Of Male Sterility And Polyploidy In Watermelon, Brent Alan Murdock

All Dissertations

No abstract provided.

Arthropods Associated With Leaf Litter Of Fraser Fir In The Great Smoky Mountains National Park, David Norman Hughes

Masters Theses

Fraser Fir, Abies fraseri (Pursh) Poiret, is a southern endemic conifer species restricted to the higher elevations of the southern Applachian mountains. This forest type is currently threatened by the balsam woolly adelgid, Adelges piceae (Ratzeburg), an exotic pest species native to Europe. This insect has destroyed more than 95% of the mature Fraser fir within the boundaries of the Great Smoky Mountains National Park (GRSM). The adelgid damages Fraser fir by disrupting the flow of nutrients in the vascular tissues of the bark. This disruption is due to abnormal growth of the vascular tissues resulting from a reaction to …

Description, Distribution, And Abundance Of The Blue Crab, Callinectes Sapidus (Rathbun), Spawning Stock Of The Lower Chesapeake Bay, Patrick John Geer

OES Theses and Dissertations

The lower Chesapeake Bay spawning stock of the blue crab, Callinectes sapidus, (Rathbun), is examined for seven years (1986 - 1992) in an attempt to better understand stock behavior. Three methods of post-stratification are used to describe the distribution and movement of the population over time. The three methods, density strata, geographic zones, and depth strata, did well in explaining movements of the population, indicating a trend of increased concentration of blue crabs near the eastern Bay late in the spawning season - October. The data suggest a bimodal period of spawning and a trimodal period of abundance. The …

Sds Non-Acrylamide Polymeric Gel-Filled Capillary Electrophoresis For Molecular Size Separation Of Protein, Devon Andres

Honors Theses

Sodium dodecyl sulfide (SDS) non-acrylamide gel-filled capillary columns are a new technology being used for analysis and separation of biotechnology-derived proteins. This research was to compare this new technology to the current methods of SDS polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance size-exclusion chromatography (HPSEC). The molecular mass of four different recombinant proteins were determined by two commercialized SDS non-acrylamide gel-filled capillary columns, SDS-PAGE, and HPSEC. The data obtained showed that the SDS non-acrylamide gel-filled capillary columns were compatible with the SDS-PAGE technique for molecular mass determination. HPSEC was shown to be unreliable for molecular weight determination. SDS non-acrylamide gel-filled capillary …

The Population Biology And Demography Of Cimicifuga Rubifolia Kearney And The Genetic Relationships Among North American Cimicifuga Species, Rebecca Ann Cook

Doctoral Dissertations

In this beginning study of the population biology of Cimicifuga species, the life history and demography of the long-lived herbaceous perennial, Cimicifuga rubifolia Kearney, were investigated, the genetic structure of some of its populations was studied, and an investigation of the genetic relationships among the North American species was begun.

The life history and demography were monitored in two populations, one of approximately 1400 individuals (1987-1990) and the second of about 400 individuals (1988-1990). A model of leaf area was used to determine the leaf area (photosynthetic size) of individuals and this was followed during the study. Relationships between the …

Comparison Of The Geographic Variation In Two Species Of Ground Squirrels (Spermophilus), Karen Marie Watts

Theses & Dissertations

Standard external measurement data was recorded from study skins at four Texas institutions. These collections also provided the specimen skulls from which twelve standard cranial measurements were made with digital calipers to the nearest 0.01 mm. This study included 111 Mexican ground squirrels (Spermophilus mexicanus) and 76 spotted ground squirrels (Spermophilus spilosoma). Most specimens had been collected in Texas and Mexico. Fifteen spotted ground squirrels had also been captured in four other states. An adaptation of the USDA Plant Hardiness Zone Map was used to subdivide the study area, so that geographic variation could be determined. Comparison of mean and …

Effects Of Portuguese Man-Of-War (Physalia Physalis) Venom On Isolated Rabbit Atria, Wayne R. Bonlie

Loma Linda University Electronic Theses, Dissertations & Projects

The cardiac effects of Portuguese man-of-war venom were studied using isolated atria from rabbits. Venom added (7.1 µg protein/ml) to the bath solution stimulates heart rate 120% (P < 0.01) and increases contraction amplitude 308% (P < 0.001). Both effects are dependent on bath concentrations of venom and calcium. The increase in contraction amplitude is significantly attenuated by diltiazem (0.8 µM) and atenolol (10 µM). Atenolol, a β1-adrenergic antagonist, completely blocks the increase in heart rate. Dantrolene (10 µM) did not significantly block the venom's effects. Ryanodine binding increases in the presence of venom showing that the venom is not in competition for calcium channels on the sarcoplasmic reticulum. These results suggest that the venom acts on the sarcolemma to increase calcium permeability, thereby causing an increase in contraction amplitude and possibly causing β1-adrenergic stimulation.

Status, Distribution And Biogeography Of The Birds Of Paraguay, Floyd E. Hayes

Loma Linda University Electronic Theses, Dissertations & Projects

The Paraguay River divides the Republic of Paraguay into two distinct regions: the Chaco to the west and the Orient to the east. The relatively flat Chaco is characterized by thorn scrub forests in the west, sub humid forests in the northeast and palm savannas in the southeast. The topographically diverse Orient is characterized by campos cerrados in the northeast, savannas in the west and southwest, and humid forests elsewhere. Paraguay's climate is subtropical, with rainfall increasing roughly fourfold from west to east.

Previous studies of the birds of Paraguay have focused primarily on the distribution and taxonomy of resident …

Inhibition Of Cytochrome P-450 But Not Neutrophil Depletion Prevents The Pulmonary Toxicity Associated With 1-Nitronaphthalene, Steven Michael Green

Masters Theses

1-Nitronaphthalene (1-NN) is an environmental contaminant that has been shown to cause pulmonary toxicity in rodents. To examine the contribution of cytochrome P-450 to the metabolism and subsequent toxicity of 1-NN, rats were pretreated with chloramphenicol, a suicide substrate of P-450. To determine if inflammation plays a role in 1-NN toxicity, rats were pretreated with cyclophosphamide to deplete neutrophils. In both experiments, morphological analysis of the lower airways was performed with light and electron microscopy. A second group of animals was examined for biochemical indicators of inflammation and cell damage. The parameters examined were cell content, protein content and lactate …

Aerotaxis In Bacillus Subtilis, Laurence Samuel Wong

Loma Linda University Electronic Theses, Dissertations & Projects

B. subtilis, like Escherichia coli and Salmonella typhimurium, responded to a step increase in oxygen concentration by swimming smoothly (~ 35 s duration) and to a decrease in oxygen by tumbling. In a spatial gradient of oxygen in liquid media, a band of cells congregated near the air interface. Aerotaxis required a functioning respiratory chain. Adaptation of B. subtilis, E. coli and S. typhimurium to media containing amino acids requires methylation of receptors/methyl-accepting chemotaxis proteins. Adaptation to oxygen and phosphotransferase sugar substrates in B. subtilis was dependent on methylation, unlike similar adaptation in E. coli and S. typhimurium. …

The Influence Of Trichinella Spiralis Infection On Heat Shock Protein 72 Production In Mrl++ Mouse Intestinal Cells, Lisa Ann Kilejian

Dissertations and Theses

The production of Heat Shock Protein 72, the inducible for~ of the highly conserved 70 kilodalton heat shock protein family, was investigated in MRL++ mouse intestine during the two weeks of a Trichinella spiralis infection. Within hours of an oral infection using the encysted Trichinella spiralis found in the diaphragm of an infected mouse, the larvae are released from the cyst in the stomach. They travel to the intestine and burrow into the epithelial layer of the intestine. The jejunum is the primary site of the intestinal phase of trichinosis (Despommier 1983). This stage of infection in the jejunum was …

White-Tailed Deer Habitat Use On Surface-Mined Areas In Eastern Kentucky, Devetta A. Hill

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Devetta A. Hill on July 2, 1993.

The Historical Transformation Of Swimming Into A Modern Leisure Activity, Stephanie J. Haas

Student Work

The purpose of this study is to trace the transformation of swimming from its earliest appearance in the literature to its contemporary form as a modern leisure activity. The processes of rationalization, legitimation and democratization are examined in pre-industrial, industrial, and post-industrial societies.

An investigation of the available literature disclosed negative attitudes toward swimming during the Middle Ages which caused a decrease in expressive uses of swimming. After this period, swimming's acceptance as a legitimate leisure activity continued to increase through the industrial and post-industrial eras. The democratization of swimming seemed limited to societies on the coasts of large bodies …

Pheromonal Composition Of Two Species Of African Amblyomma Ticks: Similarities, Differences And Possible Species Specific Components, Thomas Lem Price Jr.

Biological Sciences Theses & Dissertations

Two species of bont ticks, Amblyomma hebraeum and Amblyomma variegatum, common to Africa have been studied to compare types and quantities of compounds known or believed to serve as components of the attraction-aggregation-attachment pheromone (AAAP). A complex of attraction, aggregation and attachment pheromones are used by these ticks to detect hosts, mates and perhaps minimize interspecific breeding. Solvent extraction of pheromone emitting ticks followed by Gas Chromatography and Mass Spectrometry revealed little difference in the composition of the AAAP in these two species. However, subtle differences in the relative makeup of the pheromonal blend are noted, suggesting that such differences …

The Influence Of Temperature Salinity And Postlarval Transport On The Distribution Of Juvenile Spiny Lobsters, Panulirus Argus, In Florida Bay, Jennifer Marie Field

Biological Sciences Theses & Dissertations

Florida Bay is a major nursery for the Caribbean spiny lobster, Panulirus argus. The Bay contains a series of hardbottom or seagrass-covered basins separated by mudbanks, which serve as barriers to water circulation and presumably to transport of postlarvae. Temperatures can fluctuate dramatically in the basins and salinities range from 35 ppt to > 50 ppt. In this study I: i) investigated the physiological tolerance of spiny lobster postlarvae to combinations of temperature and salinity representative of conditions in Florida Bay, and ii) examined how postlarval availability and habitat structure influence spiny lobster recruitment into basins. I measured juvenile lobster …