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Comparison Of Restriction Fragment Length Polymorphisms In Chloroplast Dna Of Five Leafy Spurge (Euphorbia Spp.) Accessions, Scott Nissen, Robert A. Masters, Donald Lee, Martha Rowe

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Chloroplast DNA (cpDNA) restriction fragment length polymorphisms (RFLPs) were analyzed to assess genetic variation and relatedness among selections of North American and Eurasian leafy spurge. Leafy spurge accessions from Nebraska, Montana, Russia, Italy, and Austria were evaluated. Total DNA was extracted from young leaves and digested with the restriction endonuclease, EcoRI. CpDNA fragment patterns were determined by Southern blot analysis using mung bean cpDNA probes. Colinearity between the mung bean and leafy spurge chloroplast genomes was indicated by the observation that common fragments were hybridized by adjacent probes. Minimum estimates of chloroplast genome size for the five leafy spurge …