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Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
The Development Of The Vascular System In Quail Embryos: A Combination Of Microvascular Corrosion Casts And Immunohistochemical Identification, M. C. Deruiter, B. Hogers, R. E. Poelmann, L. Vanlperen, A. C. Gittenberger-De Groot
The Development Of The Vascular System In Quail Embryos: A Combination Of Microvascular Corrosion Casts And Immunohistochemical Identification, M. C. Deruiter, B. Hogers, R. E. Poelmann, L. Vanlperen, A. C. Gittenberger-De Groot
Scanning Microscopy
Although vascular casts, obtained by injection with methacrylates, are frequently used to investigate the adult vascular system, little data are available for embryonic stages. In this paper we use MercoxR in quail embryos in the period of 2 to 7 days after incubation. The microvascular corrosion casts were evaluated in the scanning electron microscope (SEM) with special attention to the development and remodelling of the large arteries and veins. Our results show that the remodelling of the large arteries and veins together with their developing tributary vessels can be visualized from very early embryonic stages onwards. However, complete replication …
Purification And Characterization Of The Prostate Cancer-Associated Antigen: 7e11-C5, Qi Feng
Purification And Characterization Of The Prostate Cancer-Associated Antigen: 7e11-C5, Qi Feng
Theses and Dissertations in Biomedical Sciences
A murine monoclonal antibody (MAb), 7E11-C5, recognizes a prostate organ-specific antigen. Under reducing conditions, the 7E11-C5 antigen exhibited major 100-Kd and minor 70-Kd bands, suggesting that the antigen comprises two peptide chains. The antigen was detected in pooled normal human seminal plasma, xenograft tissue extract of a prostate adenocarcinoma cell line, LNCaP, and tissue extracts of benign prostatic hyperplasia (BPH) and prostate carcinomas, but was not identified in any of the non-prostate tissue extracts tested. Antibodies of the three major prostate-associated antigens (prostatic acid phosphatase (PAP), prostate specific antigen (PSA) and prostate secretory protein (PSP)) were unable to block the …
Pituitary And Testicular Responses Of Beef Bulls To Active Immunization Against Inhibin Alpha, B. D. Schanbacher
Pituitary And Testicular Responses Of Beef Bulls To Active Immunization Against Inhibin Alpha, B. D. Schanbacher
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Prepubertal crossbred beef bulls served as controls or were actively immunized against the N-terminal, 30-amino acid synthetic fragment of porcine inhibin alpha, pIα,(1-30). Antibody titers were detected in sera (>40% B/Bo in sera diluted 1,000-fold) but not in rete testis fluid of 390-d-old bulls. Serum FSH and inhibin remained static during a 5-h intensive bleed; inhibin was not acutely affected by a 15-fold LH rise and a threefold FSH rise induced by exogenous GnRH. Serum FSH, but not LH or testosterone, was consistently elevated (P < .05) in immunized bulls compared with control bulls. Neither pituitary weight, pituitary gonadotropin content nor pituitary FSH/LH ratios were affected (P > .10) by pIα,(1-30) active immunization. Testicular sperm density was greater (60 x 10 …