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Full-Text Articles in Life Sciences
Practical Methods For Identification Of Rice Endosperm Protein Bodies And Fecal Protein Particles In Light Microscopy, D. L. Barber, J. N. A. Lott, D. A. Harris
Practical Methods For Identification Of Rice Endosperm Protein Bodies And Fecal Protein Particles In Light Microscopy, D. L. Barber, J. N. A. Lott, D. A. Harris
Food Structure
Indigestible 1-3 ~m protein 'cores', particles that originate from protein bodies of rice endosperm, were examined in bright-field and fluorescence microscopy, using a variety of histological and histochemical procedures. Our application of histological, histochemical and chemical approaches to a study of the feces of aoimals fed rice-containing diets has resulted in the development of methods for routine identification of insoluble rice fecal protein particles. For the specific identification of rice endosperm storage protein, whether protein bodies, cores or fecal protein particles, aqueous eosin Y stain in conjunction with fluorescence microscopy, and a combination of orange G + aniline blue in …
Extrusion-Cooking Of Pea Flour: Structural And Immunocytochemical Aspects, Hassane Ben-Hdech, Daniel J. Gallant, Brigitte Bouchet, Jacques Gueguen, Jean-Pierre Melcion
Extrusion-Cooking Of Pea Flour: Structural And Immunocytochemical Aspects, Hassane Ben-Hdech, Daniel J. Gallant, Brigitte Bouchet, Jacques Gueguen, Jean-Pierre Melcion
Food Structure
Pea flour was submitted to extrusion-cooking under various conditions. The progressive structural transformation was investigated by light microscopy and immuno- gold transmission electron microscopy. Each of the three major compounds, i.e., starch granules, protein bodies, and cell wall fragments, develop a specific, independent structure. Protein bodies aggregate and fuse giving a protein matrix. Starch granules swell, deform, come into contact with each other, and ultimately also fuse together. The resulting gel expands giving a honeycombed structure. Consequently, the protein matrix is arranged into dense strata, i.e,. protein fibers in cross or longitudinal sections, disrupted by the expanded starch gel. Cell …