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A Comparative Study Of The Myosin Light Chain Kinases From Myoblast And Muscle Sources. Studies On The Kinases From Proliferative Rat Myoblasts In Culture, Rat Thigh Muscle, And Rabbit Skeletal Muscle, S. P. Scordilis, R. S. Adelstein
A Comparative Study Of The Myosin Light Chain Kinases From Myoblast And Muscle Sources. Studies On The Kinases From Proliferative Rat Myoblasts In Culture, Rat Thigh Muscle, And Rabbit Skeletal Muscle, S. P. Scordilis, R. S. Adelstein
Biological Sciences: Faculty Publications
Myosin light chain kinases have been isolated from rat thigh and rabbit skeletal muscle and cultured rat myoblasts. From these preparations, two types of kinases can be distinguished: calcium-dependent and calcium-independent. Both types of kinases can phosphorylate isolated P-light chains of myosin from several sources (skeletal muscle, cardiac muscle, and platelet). Data are shown which support the phosphorylation of the same site on the non-muscle P-light chains by both types of kinases. The rates of these reactions are, however, different for the two types of kinases. Kinetic analysis of the myoblast kinase show differing affinities for various P-light chains (non-muscle …
Membrane Potentials And Resistances Of Giant Mitochondria: Metabolic Dependence And The Effects Of Valinomycin, Bruce L. Maloff, Stylianos P. Scordilis, Christopher Reynolds, Henry Tedeschi
Membrane Potentials And Resistances Of Giant Mitochondria: Metabolic Dependence And The Effects Of Valinomycin, Bruce L. Maloff, Stylianos P. Scordilis, Christopher Reynolds, Henry Tedeschi
Biological Sciences: Faculty Publications
The membrane potentials and resistances of giant mitochondria from mice fed cuprizone have been studied. They were found to correspond approx. 10-20 mV, positive inside, and 2 M omega, respectively. These properties were found to be independent of the metabolic state. The microelectrodes were in the inner mitochondrial space since (a) the potentials in the presence of valinomycin depended on the K+ concentration of the medium and magnitude of the K+ diffusion potentials was consistent with the presence of a high internal concentration of K+, (b) almost identical results were obtained with mitochondria from which the external membrane had been …
Assays Of The Metabolic Viability Of Single Giant Mitochondria. Experiments With Intact And Impaled Mitochondria, B. L. Maloff, S. P. Scordilis, H. Tedeschi
Assays Of The Metabolic Viability Of Single Giant Mitochondria. Experiments With Intact And Impaled Mitochondria, B. L. Maloff, S. P. Scordilis, H. Tedeschi
Biological Sciences: Faculty Publications
Single giant mitochondria isolated from mice fed cuprizone were assayed for their metabolic viability. Two tests were devised. One test optically detected the accumulation of calcium phosphate within the mitochondria under massive loading conditions (including the presence of succinate and ATP). The accumulation corresponds to a test of energy coupling from either electron transport or the hydrolysis of ATP since it is blocked by either antimycin A or oligomycin. The other assay tested for the production of ATP from ADP and Pi, using myofibrils. Myofibrils prepared from glycerinated rabbit psoas muscle contract only in the presence of ATP and not …