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Articles 1 - 10 of 10
Full-Text Articles in Life Sciences
Effect Of Glyoxylate On The Sensitivity Of Net Photosynthesis To Oxygen (The Warburg Effect) In Tobacco, David J. Oliver
Effect Of Glyoxylate On The Sensitivity Of Net Photosynthesis To Oxygen (The Warburg Effect) In Tobacco, David J. Oliver
David J. Oliver
The addition of glyoxylate to tobacco (Nicotiana tabacum) leaf discs inhibited glycolate synthesis and photorespiration and increased net photosynthetic 14CO2 fixation. This inhibition of photorespiration was investigated further by studying the effect of glyoxylate on the stimulation of photosynthesis that occurs when the atmospheric O2 level was decreased from 21 to 3% (the Warburg effect). The Warburg effect is usually ascribed to the increased glycolate synthesis and metabolism that occurs at higher O2 concentrations. Photosynthesis in control discs increased from 59.1 to 94.7 micromoles of CO2 per gram fresh weight per hour (a 60% increase) when the O2 level was …
An Enzymatic Activity In Uninfected Cells That Cleaves The Linkage Between Poliovirion Rna And The 5' Terminal Protein, Victor Ambros, Ralf Pettersson, David Baltimore
An Enzymatic Activity In Uninfected Cells That Cleaves The Linkage Between Poliovirion Rna And The 5' Terminal Protein, Victor Ambros, Ralf Pettersson, David Baltimore
Victor R. Ambros
The 5' terminal protein (VPg) on poliovirion RNA can be removed by cell-free extracts from a variety of uninfected cells. This soluble enzymatic activity is found in both nuclear and cytoplasmic extracts of heLa cells and is activated by Mg++. The enzyme activity cleaves the tyrosine-phosphate bond that links the protein to the RNA. In a partially purified form it has insufficient nonspecific protease or nuclease activity to account for its action. The existence of this enzyme implies that poliovirus RNA is translated in cell-free extracts in a form that lacks the 5' terminal protein. The role of this enzyme …
Inhibition Of Photorespiration And Increase Of Net Photosynthesis In Isolated Maize Bundle Sheath Cells Treated With Glutamate Or Aspartate, David J. Oliver
Inhibition Of Photorespiration And Increase Of Net Photosynthesis In Isolated Maize Bundle Sheath Cells Treated With Glutamate Or Aspartate, David J. Oliver
David J. Oliver
Net photosynthetic 14CO2 fixation by isolated maize (Zea mays) bundle sheath strands was stimulated 20 to 35% by the inclusion of L-glutamate or L-aspartate in the reaction mixture. Maximal stimulation occurred at a 7.5 mM concentration of either amino acid. Since the photosynthetic rate and the glutamate-dependent stimulation in the rate were equally sensitive to a photosynthetic electron transport inhibitor, 3-(p-chlorophenyl)-1,1-dimethylurea, it was concluded that glutamate did not stimulate CO2 fixation by supplying needed NADPH (NADH) through glutamate dehydrogenase. Treatment of the bundle sheath strands with glutamate inhibited glycolate synthesis by 59%. Photorespiration in this tissue, measured as the O2 …
Protein Is Linked To The 5' End Of Poliovirus Rna By A Phosphodiester Linkage To Tyrosine, Victor Ambros, David Baltimore
Protein Is Linked To The 5' End Of Poliovirus Rna By A Phosphodiester Linkage To Tyrosine, Victor Ambros, David Baltimore
Victor R. Ambros
Purification and partial characterization of the poliovirus RNA-linked protein (VPg) are described. VPg has been freed from the RNA by ribonuclease digestion and phenol extraction. Gel filtration chromatography of VPg-pUp (labeled with 32P) in 0.5% sodium dodecyl sulfate or 6 M guanidine HCl indicates that it has a molecular weight of about 12,000. VPg is bound to the 5' end of poliovirion RNA by a phosphodiester bond between a tyrosine residue in the VPg molecule and the 5'-terminal uridine. After acid hydrolysis of [3H]tyrosine-labeled VPg-pU, free tyrosine can be released by venom phosphodiesterase. Acid hydrolysis of VPg-p labeled with either …
Identification Of A Protein Linked To Nascent Poliovirus Rna And To The Polyuridylic Acid Of Negative-Strand Rna, Ralf F. Pettersson, Victor R. Ambros, David Baltimore
Identification Of A Protein Linked To Nascent Poliovirus Rna And To The Polyuridylic Acid Of Negative-Strand Rna, Ralf F. Pettersson, Victor R. Ambros, David Baltimore
Victor R. Ambros
A protein similar to that previously demonstrated on poliovirus RNA and replicative intermediate RNA (VPg) was found on all sizes of nascent viral RNA molecules and on the polyuridylic acid isolated from negative-strand RNA. 32P-labeled nascent chains were released from their template RNA and fractionated by exclusion chromatography on agarose. Fingerprint analysis using two-dimensional polyacrylamide gels of RNase T1 oligonucleotides derived from nascent chains of different lengths showed that a size fractionation of nascent chains was achieved. VPg was recovered from nascent chains varying in length from 7,500 nucleotides (full-sized RNA) to about 500 nucleotides. No other type of 5' …
Notes On The Flora Of Rhode Island, Gordon C. Tucker
Notes On The Flora Of Rhode Island, Gordon C. Tucker
Gordon C. Tucker
No abstract provided.
Distribution And Relative Abundance Of Small Mammals In The Illini Forest Plantation, Urbana, Illinois, James M. Novak
Distribution And Relative Abundance Of Small Mammals In The Illini Forest Plantation, Urbana, Illinois, James M. Novak
James M. Novak
The abundance and distribution of small mammals were analyzed for correlation with the vegetational characteris· tics of their habitat. Positive correlations were found for the number of mammals trapped per area trapped versus the total ground cover (r= .67, P< .05) and for the number of mammal species versus total ground cover (r= .73, P<.05). The habitat preferences of the three species trapped (short-tailed shrews B/,arina brevicauda. white-footed mice Peromyscus leucopuus, and meadow voles Microtus pennsylvanicus) showed a distinct distributional pattern for the species in relation to their habitats. The management of forest-tree plantations to achieve high densities during the seedling stage and the use of silvicultural practices that promote high shrub densities are suggested as inexpensive and natural control measures for injurious microtine rodents. Seedlings must be permitted to reach a sufficient height to remove the risk of the shrubs overtopping them.
Breadbox Water Heater Designs, David A. Bainbridge
Breadbox Water Heater Designs, David A. Bainbridge
David A Bainbridge
Integral solar water heaters (batch, breadbox, ICS) provide the most economical and reliable means of heating water. Simple designs can perform well and provide service for decades. Basic design principles include box insulation, orientation and control of heat loss.
Ethological Considerations In The Experimental Study Of Lizard Behavior, Neil Greenberg
Ethological Considerations In The Experimental Study Of Lizard Behavior, Neil Greenberg
Neil Greenberg
The importance of an ethological approach to the experimental study of an unfamiliar species is described and several of its problems discussed. The selection of units of behavior is a crucial first step in the development of a behavior inventory. The correlation of a behavioral unit with a particular context is necessary to ascribe function to that to that unit and to develop an ethogram. Methods of studying lizards under controlled conditions are described and discussed. Constraints on behavior that must be considered in an experimental study include the microclimate and its thermal qualities, food and water, shelter utilization, and …
A Movement Barrier Useful In Population Studies Of Small Mammals, F Cole
A Movement Barrier Useful In Population Studies Of Small Mammals, F Cole
F. Russell Cole
No abstract provided.