Life Sciences Commons^™

Ua77/1 Western Alumnus, Vol. 49, No. 6, Wku Alumni Association

WKU Archives Records

Alumni magazine published by WKU. This issue has the following articles:

• Dero Downing Steps Down
• Douglas, Michele. Top Banana – Gary Riggs
• Egan, Sherry. WKU State Climatic Center
• Gibson, Debbie. I Kind of Believe It’s a Gift – Burt Feintuch, Folk Music
• New on the Gridiron
• Adams, Bob. Digital Voltmeter – Engineering Technology
• Schock, Jack. The Projectile Point – Archaeology
• Armstrong, Bryan. Confessions of a Journalism Intern
• Highland, Jim. Terry Climer
• Beauchamp, Donnie. Commonplace Things – Photography
• Miller, Richard. The Exceptional Student – Psychology, Biology
• Harry Snyder Speaks at Commencement
• Douglas, Michele. Students Take Advantage of Study Trips Abroad
• Western …

The Effect Of Parental Bone Marrow & Spleen Cells In The Induction Of The Graft-Versus-Host Reaction, Ronald Grane

Masters Theses & Specialist Projects

Variously aged (C57BL X C3H)F₁ hybrid mice were used as recipients in experiments to determine if spleen or bone marrow cells from normal C57BL or C3H donors, spleen or bone marrow cells from C57BL donors previously treated with cortisone, or C57BL bone marrow cells previously incubated with serum from X-irradiated C57BL mice could induce a graft-versus-host reaction (GVHR).

The mice were assayed for GVHR by mortality or splenomegaly as detected by the discriminant spleen assay developed by Simonsen. Results show that spleen cells from normal C57BL, normal C3H, or cortisone-treated C57BL mice are capable of inducing a GVHR in …

Synergistic Effects Of Wavelengths Of Ultraviolet Radiation On Capacity In A Mammalian Cell-Virus System In Vitro, Bobby Cobb

Masters Theses & Specialist Projects

The synergistic effect between each of two minor contaminating UV wavelengths has been studied. The TC-7 host cell-capacity system was used. The macromolecular damage induced in monolayers of cell cultures as the result of exposure to UV radiation was measured by determining the ability of irradiated cells to support the replication of Herpes simplex virus. The macromolecule involved has been shown to be DNA and the damage is probably due to the formation of thymine dimers plus some undefined chromosphore associated with the DNA of the cell.

Preliminary experiments showed that low exposures of germicidal radiation are less effective in …

Ecotypic Differentiation In Ohio & Mississippi Populations Of Acer Negundo L., Anthony Greco

Masters Theses & Specialist Projects

Laboratory investigations of Acer negundo populations from two widely separated habitats revealed distinct patterns of ecotypic differentiation in budbursting, leaf fall, and chlorophyll levels. Second year seedlings of Mississippi seed origin had complete leaf emergence before any evidence of budbursting in the Ohio progeny of similar age. Leaf fall patterns showed a clear difference between the two populations with Ohio plants dropping their leaves 2.5 weeks prior to complete defoliation in the Mississippi progeny. Significant differences in chlorophyll levels were demonstrated with the more northern (higher latitude) Ohio population showing consistently greater amounts of chlorophyll per gram dry weight than …

Quantitative And Qualitative Analysis Of The Periphyton Of Sloan’S Crossing Pond, Mammoth Cave National Park, Kentucky, Judith Orser

Masters Theses & Specialist Projects

Periodic collections were obtained from Sloan’s Crossing Pond, Mammoth Cave National Park, for one year to analyze, quantitatively and qualitatively, the periphyton community and determine patterns of periodicity. Productivity was measured by dry and ash-free dry weights and chlorophyll a content.

Productivity was lowest during the winter months and highest between July and September. Dry weight showed a range of 3.0-243 mg/m­²/day, the peak occurring in August. Ash-free dry weight also reached its annual peak in August at 92 mg/m²/day. Chlorophyll a followed the pattern of dry weight except its peak was obtained in July at …

The Effect Of Spleen Cells From F1 Hybrid Mice Undergoing A Graft-Versus Most Reaction Upon The Humoral Immune Response Of Parental Mice, Elizabeth Schmidt

Masters Theses & Specialist Projects

Mice undergoing a graft-versus-host reaction (GVHR) exhibit a surppressed immune response. Cells responsible for this suppression have been found to be localized in the spleen. Thus, in this research, a study was made to evaluate the effect of spleen cells from F­₁ mice undergoing a GVHR upon the humoral immune response to sheep erythrocytea (SRBC) in parental mice. An attempt was made to create an experimental model involving the transfer of suppressor cells in vivo. Parental BALB/c spleen cells were injected into (C57BL X BALB/c)F₁ hybrids to induce a GVH disease. Ten or twenty days later, spleen …

An Analysis Of The Heavy Metal Content Of The Scales Of Several Fishes In Southwestern Kentucky, Thomas Dahl

Masters Theses & Specialist Projects

Scales of the stoneroller, Campostoma anomalum (Rafinesque), common shiner, Notropus cornutus (Mitchill), and the bluntnose minnow, Pimephales notatus (Rafinesque) were analyzed by means of atomic absorption spectrophotometry to determine levels of cadmium, calcium, copper, iron, lead, manganese, nickel and zinc. Metal concentrations were determined seasonally and relationship established between scale metal content and environmental water metal levels.

Calcium, iron manganese and zinc were found in all samples analyzed. Cadmium, copper, lead and nickel were not observed in measurable quantities.

Metal concentrations varied interspecifically, but most showed little fluctuation in response to increased metal content of the water. Elemental composition of …

The Induction Of Thy -1.2 Antigen Expression In Murine Bone Marrow Cells Incubated In Vitro With Immune Serum, Terry Knight

Masters Theses & Specialist Projects

Adult BALB/c mice were immunized with C57BL/6 spleen cells first on day 0 and again on day 8 of the experimental procedure. Serum was collected from these animals on day 0 (before the first immunization), day 4, day 8 (before the second immunization), day 12, and day 16. Two groups of normal BALB/c bone marrow cell suspensions were incubated with the experimental immune sera: and one of the groups of cell suspensions was subsequently incubated with bacterial lipopolysaccharide. Following the incubation steps, both groups of cell preparations were assayed for their ability to absorb the activity of the anti-Thy-1.2 serum. …

The Wavelength Dependence Of Ultraviolet Enhanced Reactivation In A Mammalian Cell-Virus System, Leslie C. James

Masters Theses & Specialist Projects

The effect of ultraviolet (UV) radiation in the wavelength region 230 nm to 302 nm on the ability of an irradiated mammalian cell to reactivate UV-irradiated mammalian virus was tests. An action spectrum for radiation enhanced reactivation (RER) Is presented. The shape of the action spectrum points to a combined nucleic acid protein target for UV radiation effects on this cellular parameter. An analysis of the results of others involving the biochemical and photobiological events involved in the RER does not allow the identification of the macromolecule which is the major contributor to this effect. Studies involving an analogous phenomenon …

The Wavelength Dependence Of Ultraviolet Induction Of Simian Virus 40 From A Transformed Line Of Hamster Kidney Cells, Dennis Fry

Masters Theses & Specialist Projects

Ultraviolet light has previously been shown to cause viral induction in some SV40 transformed cell lines. The induction of this virus from an established line (clone E) of hamster kidney cells by monochromatic ultraviolet light was studied. Ten wavelengths in the region of 240 nm to 366 nm were tested. The induced viral titers were determined by the agar overlay plaque assay technique.

A distinct wavelength dependence was shown for induction in this cell-virus system. A broad peak in induction efficiency extending from 260 nm to 280 nm was found. This indicates a DNA-protein interaction in the induction process.