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Full-Text Articles in Life Sciences
Labeling Of Glutamate, Glutamine, And Aspartate In Brain From C14 Aspartate And Acetate / W. J. Ncklas D. D. Clarke And S. Berl Chemistry Dept., Fordham University And Col. Of Physicians And Surgeons, Columbia University, William J. Nicklas, Donald Dudley Clarke Phd, Soll Berl
Labeling Of Glutamate, Glutamine, And Aspartate In Brain From C14 Aspartate And Acetate / W. J. Ncklas D. D. Clarke And S. Berl Chemistry Dept., Fordham University And Col. Of Physicians And Surgeons, Columbia University, William J. Nicklas, Donald Dudley Clarke Phd, Soll Berl
Chemistry Faculty Publications
Guinea pig brain cortex slices incubated in media containing U-C14 aspartic acid form glutamine of higher specific activity than glutamic acid (Fed. Proc. -25, 713 (1966) ). Glutamic acid and glutamine from such experiments were enzymatically decarboxylated and the o/o of label in C-1 ranged from 37 to 42o/o. These values exceed the maximum predicted from the known operation of the citric acid cycle viz. 33o/o. When l-C14 acetate was injected into mice or incubated with brain slices the aspartate isolated from brain had more than 50o/o of the label in C-4. From the known operation of the citric acid …
Determination Of Urinary 3-Methoxy-4-Hydroxyphenylethylene Glycol By Gas-Liquid Chromatography And Electron Capture Detection / Sherwin Wilk, Stanley E. Gitlow, Donald D. Clarke And David H. Paley Department Of Medicine, The Mount Sinai Hospital, New York, And Department Of Chemistry, Fordham University, New York, N.Y. (U.S.A.), Sherwin Wilk, Stanley E. Gitlow, Donald Dudley Clarke Phd, David H. Paley
Determination Of Urinary 3-Methoxy-4-Hydroxyphenylethylene Glycol By Gas-Liquid Chromatography And Electron Capture Detection / Sherwin Wilk, Stanley E. Gitlow, Donald D. Clarke And David H. Paley Department Of Medicine, The Mount Sinai Hospital, New York, And Department Of Chemistry, Fordham University, New York, N.Y. (U.S.A.), Sherwin Wilk, Stanley E. Gitlow, Donald Dudley Clarke Phd, David H. Paley
Chemistry Faculty Publications
The determination of urinary 3-methoxy-4-hydroxyphenylethylene glycol (HMPG), a major metabolite of epinephrine and norepinephrine by gas-liquid chromatography and electron capture detection is described. A purified extract of HMPG was treated with trifluoroacetic anhydride to form the highly sensitive HMPG-trifluoroacetate derivative. Chromatography was performed on a 6% QF-I- 0065 column at 158°. Recovery was found to be 55·4% using [3H]HMPG. Two sets of eight identical samples demonstrated a standard deviation from the mean of 6.9% and 5.7%. Thirty-five normal human urine specimens assayed by this procedure gave a mean HMPG excretion of 0.86 #gfmg creatinine (range 0.25-1.49). Six urine samples from …
Carbohydrate And Lipid Metabolism During Germination Of Uredospores Of Puccinia Graminis Tritici, J. M. Daly, H. W. Knoche, M. V. Wiese
Carbohydrate And Lipid Metabolism During Germination Of Uredospores Of Puccinia Graminis Tritici, J. M. Daly, H. W. Knoche, M. V. Wiese
Department of Biochemistry: Faculty Publications
Uredospores of Puccinia gramintis (Pers.) tritici (Eriks. and Henn.) were uniformly labeled with 14C by permitting the host (Triticuml aestivum L.) to carry out photosynthesis in 14CO2 during the process of spore production by the obligate parasite. The use of 14C labeled spores provided advantages in a study of the utilization of endogenous substrates at frequent intervals with small amounts of spores under conditions conducive to germination.