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Full-Text Articles in Life Sciences
Lions, Tigers, And Hemes - Oh My! A Dynamic Look At The Electronic Effects Of Porphyrin Substitution On Cytochrome P450 Olet, Alexis J. Holwerda
Lions, Tigers, And Hemes - Oh My! A Dynamic Look At The Electronic Effects Of Porphyrin Substitution On Cytochrome P450 Olet, Alexis J. Holwerda
Senior Theses
OleT, a member of the CYP152 family of cytochrome P450s (CYPs), decarboxylates fatty acids using hydrogen peroxide as an oxidant. The resultant products are a terminal alkene and carbon dioxide. This C–C cleavage reaction is highly atypical for CYPs, which prototypically oxygenate substrates, and provides a potential means to enzymatically produce drop-in fuels. OleT contains a heme-iron cofactor that facilitates decarboxylation through the activation of hydrogen peroxide. The catalytic cycle, as determined by transient kinetics, includes two ferryl intermediates known as Compound I (Ole-I) and Compound II (Ole-II). Ole-I performs substrate hydrogen abstraction and subsequent single electron transfer to Ole-II …
Effect Of Ph And Lipid Composition On Membrane-Spanning Helices With Glutamic Acid Examined By Solid-State Nmr, Kelsey Marr
Effect Of Ph And Lipid Composition On Membrane-Spanning Helices With Glutamic Acid Examined By Solid-State Nmr, Kelsey Marr
Chemistry & Biochemistry Undergraduate Honors Theses
Transmembrane proteins constitute about 30% of the proteins in a mammalian cell and are involved in major biological processes. The dynamic properties of membrane proteins and the ionization states of particular side chains are important for biological function. The biophysical properties of membrane proteins nevertheless can be difficult to decode, particularly for glutamic acid in the lipid environment of cell membranes. To study the ionization of glutamic acid in transmembrane peptides, guest glutamic acid residues were substituted into the well-defined model helix of GWALP23 (acetyl-GGAL4WLALALALALAL16ALWLAGA-amide). These guest residues were placed at position L16 or L4 and specific 2H-labeled alanine residues …
Structural Studies On Calcium/Calmodulin-Dependent Activation Of Eukaryotic Elongation Factor 2 Kinase, Kwangwoon Lee
Structural Studies On Calcium/Calmodulin-Dependent Activation Of Eukaryotic Elongation Factor 2 Kinase, Kwangwoon Lee
Dissertations, Theses, and Capstone Projects
Eukaryotic elongation factor 2 kinase (eEF-2K) is a key modulator of the rate of protein synthesis. Activated by calcium-loaded calmodulin (Ca2+-CaM), eEF-2K phosphorylates its only known physiological substrate, eEF-2, on a specific threonine residue (Thr-56). Phosphorylated eEF-2 has reduced affinity for the ribosome, and results in a significant decrease in the rate of translation elongation. Modulation of the rate of translation elongation plays a crucial role in proteostasis – adequate regulation of protein synthesis, protein folding, and protein degradation that greatly influences cellular growth and survival. Binding of Ca2+-CaM triggers activation of eEF-2K and remains intact …
Stabilization Of Dna I-Motif Structures By 7-Aminoactinomycin D, An Anti-Tumor Drug, Justin Lane Parmely
Stabilization Of Dna I-Motif Structures By 7-Aminoactinomycin D, An Anti-Tumor Drug, Justin Lane Parmely
Electronic Theses and Dissertations
Alternative DNA structures are likely to form from Watson-Crick B-form DNA when antitumor drug known to bind DNA loops -- can affect the iM structure. Our results demonstrate as an i-motif (iM). While both structures are known to exist in vivo they are energetically uphill can utilize intercalating cytosine-cytosine base pairing to form a four-stranded structure known controlled by alternative DNA structures like G4s and iMs. especially during processes that involve superhelical duress. A guanosine rich strand can form a facilitate their stabilization. In this report we present data on how 7-aminoactinomycin D -- an formation. Earlier it was believed …
The Beta-Catenin/Muc1.Ct Interaction In Pancreatic Cancer, Edwin Wiest
The Beta-Catenin/Muc1.Ct Interaction In Pancreatic Cancer, Edwin Wiest
Theses & Dissertations
MUC1 is overexpressed in over 90% of pancreatic cancer cases, and its interaction with beta-catenin promotes progression of the disease. Various in vitro and in vivo methods show that beta-catenin and MUC1 interact by way of the cytoplasmic tail of MUC1 (MUC1.CT). This interaction occurs in the membrane of pancreatic cancer cells but is found to a smaller extent in the nucleus as well. Biophysical methods suggest that MUC1 interacts with beta-catenin through a sequence of amino acids in the tail of MUC1 that sit very near the transmembrane domain of MUC1. In pancreatic ductal adenocarcinoma cells, it appears that …
Interactions Between Phospholipids And Polymers With Time Resolved Fluorescence Spectroscopy, Zachary E. Hier
Interactions Between Phospholipids And Polymers With Time Resolved Fluorescence Spectroscopy, Zachary E. Hier
Williams Honors College, Honors Research Projects
Interactions between macromolecule species and fluorescently labeled phospholipids were explored in supported lipid bilayers (SLBs). The concentration and the lateral mobility of the labeled phospholipids within the confocal volume were determined via fluorescence correlation spectroscopy. Low molecular weight polystyrene was used for the neutral charged macromolecule. Three separate concentrations of polystyrene were used and a significant change in lateral mobility of phospholipids was found for the SLB doped with all three concentration of styrene from polystyrene (molecular weight = 1000). Polyester with mimic lysine pendant groups was used for the cationic polymer at physiological pH. A large decrease in the …
Investigating The Flexibility Of Intrinsically Disordered Proteins In Folding And Binding, Amanda Leilah Debuhr
Investigating The Flexibility Of Intrinsically Disordered Proteins In Folding And Binding, Amanda Leilah Debuhr
Chancellor’s Honors Program Projects
No abstract provided.