Life Sciences Commons^™

Investigation Of The Mechanism Of Binding Of Perfluoroalkyl Acids With Human Serum Albumin Using An Improved Approach To Equilibrium Dialysis, Michael Morris

Honors Theses

Perfluoroalkyl acids (PFAAs) are used to produce many consumer products, but their bioaccumulative and toxic properties and their global persistence in the environment are major concerns. In particular, PFAAs have been shown to accumulate in the blood, liver, and kidneys of organisms. As such, it is important to elucidate the toxicokinetics of PFAAs by quantitatively and qualitatively characterizing the binding mechanism of PFAAs to human serum albumin (HSA). In this study, the binding of PFAAs to HSA are studied via a high-throughput equilibrium dialysis method that utilizes the 96-well Equilibrium Dialyzer coupled with sample quantitation using liquid chromatography tandem-mass spectrometry …

Effects Of Rumen-Protected Amino Acids On Production In Holstein Cows, David Paul Dawson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Six nonlactating Holstein cows fitted with rumen and duodenal cannula were used to determine the efficacy of pH sensitive fatty acid polymer encapsulation as a means protecting amino acids from rumen fermentation and as a post-ruminal amino acid delivery system. The cows were arranged in a 2 X 2 factorial in a Latin Square design. Treatments were 1) the basal ration, and 2) basal ration plus rumen-protected lysine, methionine, and threonine at 10 g each per day.

Rumen parameters measured were pH, ammonia, volatile fatty acids, protozoa, liquid, and dry matter rate of passage, total viable bacteria, and viable cellulolytic …