Life Sciences Commons^™

Food Safety And Functionality Assessment Of Kombucha Systems Through Bacillus Cereus Spore And Probiotic Inoculations, Alexandria L. Bromley

Electronic Theses and Dissertations

Kombucha is a SCOBY-fermented tea beverage known for its taste, sensorial qualities, and high endogenous microbial load. In recent years, kombucha has become a popular functional food with a compound annual growth rate of 25% from 2015 to 2020 in American kombucha sales alone, and sales are predicted to keep increasing significantly over the next decade. However, kombucha is lacking in scientific research, and there is still much to be explored regarding its safety and native probiotic content. This research looked into the various routes of contamination of several kombucha systems as well as the feasibility of the producing a …

Molecular, Somatic, And Performance Characteristics Of Broilers Exhibiting Woody Breast Myopathy, And The Effects Of Dietary And Challenge Intervention Strategies, Linan Jia

Theses and Dissertations

Woody breast (WB) is a meat quality problem that has caused significant economic losses for the poultry industry. Ross × Ross 708 chicks were randomly assigned to a 3 (diet) × 2 (cocci challenge) × 2 (sex) factorial arrangement of treatments. The three diets included the control diet (corn-soybean meal basal diet), antibiotic diet (basal diet + 6.075 mg bacitracin /kg feed), and probiotic diet (basal diet + 2.2 × 108 CFU Bacillus subtilis PB6 /kg feed). Birds in the cocci challenge treatment group received 20 × the live cocci vaccine as an inoculum on d 14. Growth performance and …

The Effects Of Probiotic Therapy On Stress Coping Styles Of Triploid Juvenile Farmed Chinook Salmon, Oncorhynchus Tshawytscha, Brendyn M. St. Louis

Electronic Theses and Dissertations

Triploid salmon – fish with three sets of chromosomes – are regularly used in aquaculture as they are functionally sterile and therefore avoid the decline in flesh quality associated with sexual maturation. However, triploid fish exhibit many immune, behavioural, and physiological drawbacks which reduce their condition and survival in aquaculture environments. My research examines the effects of probiotic treatment to promote behavioural and stress-related (termed ‘coping styles’) traits more suited for aquaculture environments in triploid fish with the ultimate goal of increasing fish yield. In this study, Chinook salmon (Oncorhynchus tshawytscha) crosses were developed in a 3x3 and 3x2 design, …

Fishing For The Right Probiotic: Investigating The Mechanism Of Mucosal-Bacterial Interactions At The Interface Of Health And Productivity In Salmonid Aquaculture, Luana Langlois

Electronic Thesis and Dissertation Repository

Aquaculture is vital for the global food supply, but the high incidence of infectious diseases threatens the industry’s productivity. The intestinal mucosa is a key port of entry for pathogens and provides an extensive interface for host-microbe interactions. Tight junctions are at the core of gut barrier function and the mucosal health of finfish. Disruption of these complexes gives rise to sepsis, which leads to systemic inflammation and death. The present study employs a combinatorial approach that integrates in vitro and in vivo analyses to gain actionable insights into the mechanism of microbial-mediated modulation of host health. The experiments outlined …

Microbial Innovations For Disease Management In Honey Bees, Brendan A. Daisley

Electronic Thesis and Dissertation Repository

Arguably the most important insect pollinator, honey bees (Apis mellifera) are threatened by infectious disease, pesticide exposure, and nutritional deficits resulting from habitat loss.

The major goals of this thesis were to advance our understanding of the immune and microbiota factors underpinning synergistic interactions between the threats, and to develop a honey bee-specific probiotic with strain-level functions to alleviate cumulative stress burden.

Drosophila melanogaster was used as a high-throughput discovery platform in molecularly characterizing the effects of candidate probiotic lactobacilli and neonicotinoid-neuroimmune-microbiota mechanisms prior to experimentation in honey bees. Imd pathway activation by lactobacilli was identified to be …

The Probiotic Effect Of Clostridium Cochlearium Is Associated With Significant Change In Short-Chain Fatty Acid Metabolism And Gut Microbiota, Qing Ai

Wayne State University Dissertations

The prevalence of obesity is rising steadily across the world, which increases the risk of many metabolic diseases and life-threatening illnesses. A specific strain, Clostridium cochlearium (C. cochlearium), reported as a butyrate producer, could have potential probiotic effects against obesity. The objective of this study was to evaluate the effects of dietary supplementation of C. cochlearium on a high-fat diet-induced obese (DIO) mouse model. The 16S rRNA sequencing of mice gut microbiome was performed at the end of the experimental period to identify the changes in gut microbial composition, investigate possible functional genes, and elucidate potential mechanisms. Thirty-six C57BL/6 6-8 …

Developing Whey Protein Encapsulated Probiotics And Evaluating Environmental Listeria Using Whole-Genome Sequencing, Shayanti Minj

Electronic Theses and Dissertations

In this study, a novel value-added dairy-based health formulation was developed using whey protein hydrolysate and probiotic organisms. In the first part of the study, different forms of whey proteins, concentrate WPC80, isolate WPI90, and hydrolysates WPH10, WPH15 and WPH20 were screened for bioactivities (antimicrobial, antioxidant, and antihypertensive activity). Hydrolysate WPH10, exhibiting the highest bioactivities was conjugated with maltodextrin. A batch of 2L conjugated solution was spray dried in a Niro drier with an inlet and outlet temperature of 200⁰C and 90⁰C, and alternatively, freeze dried at −80 °C under 50 mTorr vacuum. The bioactivities of the conjugated samples were …