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Full-Text Articles in Life Sciences

The Role Of A-Layer In Polyunsaturated Fatty Acid (Pufa)-Mediated Effects On Aeromonas Salmonicida Subsp. Salmonicida, Allen Lin Dec 2021

The Role Of A-Layer In Polyunsaturated Fatty Acid (Pufa)-Mediated Effects On Aeromonas Salmonicida Subsp. Salmonicida, Allen Lin

Honors Theses

Aeromonas salmonicida subsp. salmonicida is a Gram-negative bacterium that infects salmonids and non-salmonids worldwide leading to an infection known as furunculosis, which is characterized by skin lesions and hemorrhages of the fish epidermis. This infection is carried out by a A+ (virulent strain) of A. salmonicida containing an important virulent factor known as the A-layer, which is a 2D paracrystalline structure that binds to the basement membrane and functions to promote adherence to host membranes and resistance to host defense. The purpose of this study was to examine the ability of the A+ A. salmonicida to incorporate exogenous fatty acids …


Examining An Intersection Of Environmental Justice And Covid-19 Risk Assessment: A Review, Ashley Ellis Dec 2021

Examining An Intersection Of Environmental Justice And Covid-19 Risk Assessment: A Review, Ashley Ellis

Honors Theses

This study views the risks associated with the novel coronavirus (COVID-19) as an environmental injustice issue due to the connection between existing environmental disparities and the disproportional negative impacts brought upon by the virus. The social and health determinants attributed to those environmental disparities have traditionally been evaluated as individual risk factors, an approach that fails to gauge the complexity of an environmental injustice issue. This study employs the emerging theory of intersectionality, a belief that phenomena cannot be linked to one principal cause but instead an interconnected web of influences, in order to synthesize the multitude of factors believed …


Calibration Study Of A Bacterial Quantification Technique Using Transport Swabs And Line Inoculations On Agar Growth Medium, Seth Larue Aug 2021

Calibration Study Of A Bacterial Quantification Technique Using Transport Swabs And Line Inoculations On Agar Growth Medium, Seth Larue

Honors Theses

Enumeration of bacteria has been the subject of research for over a century. Some of the techniques that have been developed include viable plate counts, the most probable number assessment, and more modern molecular techniques. Many of the newer molecular-based techniques provide little or no information about the viability of the cells being counted, and are generally not quantitative. Having quantitative data for potentially pathogenic bacteria on surfaces can be very useful in many settings, particularly in healthcare facilities. Knowing the numbers of bacterial cells present on surfaces within healthcare facilities may be important to attempt to study the cause …


Troglofauna Of Van Buren And White County: A Survey Of Four Caves In The Mideastern Cumberland Plateau, Gavin Page May 2021

Troglofauna Of Van Buren And White County: A Survey Of Four Caves In The Mideastern Cumberland Plateau, Gavin Page

Honors Theses

The Cumberland Plateau in Tennessee has the highest cave-obligate biodiversity in North America. Cave-obligate species are entirely restricted to subterranean habitat and demonstrate unique troglomorphy. These species are categorized as troglobionts. However, because of their isolated occurrences, theses species are vulnerable to a variety of disturbances. This issue is furthered in the fact that only approximately 7% of the caves in this region have been surveyed. Of these caves that have been surveyed, only a small portion were professional entomological surveys, while almost no cave has ever been extensively repeat sampled. This study involved newly sampling one cave and repeat …


The Effect Of The Apolipoprotein A1 (Apoa1): The Stability And Folding In Potassium Chloride Environment, Alexandra Paladian May 2021

The Effect Of The Apolipoprotein A1 (Apoa1): The Stability And Folding In Potassium Chloride Environment, Alexandra Paladian

Honors Theses

Healthy levels of potassium chloride (KCl) can significantly affect the workings of the cholesterol level of the human body and how they pertain to an individual person. The search for a better salt additive for the human diet can provide a better option for people who experience high cholesterol levels and heart disease. The study focuses on the experimental design of the Molecular Dynamic (MD) simulation of the Apolipoprotein A1 (APOA1) in the potassium ion solution environment to determine the stability and folding of the protein. The study also compares its data to the previous experimental design of chloride ions …


An Analysis Of The Pollinators Of Echinacea Purpurea In Relation To Their Perceived Efficiency And Color Preferences, Carmen Black May 2021

An Analysis Of The Pollinators Of Echinacea Purpurea In Relation To Their Perceived Efficiency And Color Preferences, Carmen Black

Honors Theses

This study aimed to better understand how insects interacted with species of Echinacea in Tennessee and specifically their preference to floral color. Based on previous studies I expected the main visitors to be composed of various bees, beetles and butterflies. Based on previous studies, I hypothesized that most bees (especially social bees) would most likely pollinate the purple/violet morphs of the flower versus the white morph. I also expected the bees would have the most visits to the coneflowers versus any other taxonomic group. I believe bees to be the majority of pollinators based upon the results of Stucky, Gadd, …


Retrospective Analysis Of Injuries And Persisting Injury Effects Among Students Who Have Participated In Club Sports And Intramural Activities During The Prior Year, Daylia Wallace May 2021

Retrospective Analysis Of Injuries And Persisting Injury Effects Among Students Who Have Participated In Club Sports And Intramural Activities During The Prior Year, Daylia Wallace

Honors Theses

Research suggests that previous injury and history of sport-related concussion place athletes at a greater risk for subsequent injury. The primary purpose of this study was to assess the validity of self-perceived wellness and performance capabilities survey responses for retrospective classification of the injury risk status of individual athletes as a means to estimate risk magnitude for intramural and club sport athletes. The surveys used to collect this data were the Sport Fitness Index (SFI), Overall Wellness Index (OWI), Depression, Anxiety, and Stress Survey (DASS), and the Pittsburgh Sleep Quality Index (PSQI). The SFI was a strong discriminator (AUC= .728) …


The Effects Of Urbanization On Insect Morphology: A Meta-Analysis, Evann L. Bailey May 2021

The Effects Of Urbanization On Insect Morphology: A Meta-Analysis, Evann L. Bailey

Honors Theses

Urbanization has been shown to create a rapid change in the environment as you move from rural areas to urban areas. It can create a multitude of effects on the environment. Some examples include, land disturbance, pollution, increasing temperatures and a disturbance in vegetation and biodiversity. Insects are useful organisms that provide maintenance and upkeep for ecosystem functioning. The rapid development of urbanization and how it is changing the environment may impact insect morphology. Measuring morphological change in organisms have been used successfully as indicators of environmental and ecological disturbance. Changes that take place in an insect’s morphology may indicate …


A Metabolomics-Based Approach To The Screening Of Endometrial Cancer: Development Of A Gas Chromatography-Ion Trap Mass Spectrometry-Based Method, Allycia Lee May 2021

A Metabolomics-Based Approach To The Screening Of Endometrial Cancer: Development Of A Gas Chromatography-Ion Trap Mass Spectrometry-Based Method, Allycia Lee

Honors Theses

Endometrial Cancer (EC) is the most common malignant tumor of the female reproductive tract. Currently there are no methods for effectively screening EC, but one method that is thought to be useful in screening EC is metabolomics. Metabolomics involves the analysis of metabolites which are low molecular weight inorganic and organic chemicals that are substrates, intermediates, and by-products of enzyme-mediated biochemical reactions in the cell. Previous research shows that the metabolic signature of EC patients are discernable from those of healthy patients. To aid in the development of an effective screening method for EC, blood serum, urine, and saliva samples …


Molecular Dynamic Simulation Of The Complex Folding Patterns Of Apolipoprotein A1 In Various Concentrations Of Potassium Chloride, Hannah Holmberg May 2021

Molecular Dynamic Simulation Of The Complex Folding Patterns Of Apolipoprotein A1 In Various Concentrations Of Potassium Chloride, Hannah Holmberg

Honors Theses

Apopliprotein or ApoA-1 is a complex lipoprotein that functions in the removal of cholesterol from the blood, removing cholesterol from the area around white blood cells and promoting the excretion of lipids through the lymphatic system. Previous research has found that ApoA-1 shows both folded and unfolded conformations depending on the concentration of NaCl in solution in the water around it. The protein was studied using molecular dynamics simulations. Once this state of equilibrium was reached, various structural properties of the protein were measured including the radius of gyration and the radial distribution function. The goal of the project was …


Increasing Stress Tolerance In Saccharomyces Cerevisiae Using Lipocalin Genes, Ximena Leon May 2021

Increasing Stress Tolerance In Saccharomyces Cerevisiae Using Lipocalin Genes, Ximena Leon

Honors Theses

Saccharomyces cerevisiae is a microorganism that is commonly used in industries, but its productivity is significantly hindered by its inability to resist high stress levels found in industrial processes. By increasing stress tolerance in S. cerevisiae, its role in industrial processes could be maximized. The objective of this study is to determine if complementation of lipocalin genes into the wildtype and knockout strains of S. cerevisiae can increase stress tolerance against a variety of stressors. We identified the knockout yeast strains, ALD3, ALD4, PDX3, and ILV1, as being sensitive to various stressors including salinity, heat, oxidative, and osmotic stresses. Arabidopsis …