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Full-Text Articles in Life Sciences
Virology: Mutagenizing The Proteolytic Cleavage Site In The Major Capsid Protein In Ssv1, Thejanee Liyanaarachchi
Virology: Mutagenizing The Proteolytic Cleavage Site In The Major Capsid Protein In Ssv1, Thejanee Liyanaarachchi
University Honors Theses
SSV1, spindle-shaped virus 1, is an archaeal virus with a unique shape that belongs to the family of fuselloviruses and infects hyperthermophilic archaea that thrive at 80°C and pH 3. SSV1 has two capsid proteins, VP1 and VP3, that presumably produce the lemon-shaped virion structure characteristic of fuselloviruses. The VP1 amino acid sequence is highly conserved among SSVs. Here, we investigate the importance of the proteolytic cleavage site in position E66 in VP1 by changing its glutamic acid to the other 19 amino acids. We have developed a new protocol for creating SSV1 mutants through HiFi Assembly (NEB) to increase …
A Bioinformatic And Biochemical Analysis Of Cruciviruses, George William Kasun
A Bioinformatic And Biochemical Analysis Of Cruciviruses, George William Kasun
Dissertations and Theses
Cruciviruses are novel ssDNA viruses discovered through metagenomics and direct environmental DNA amplification and cloning. The genomes of cruciviruses suggest that gene transfer between RNA and DNA viruses occurred due to the presence of putative protein-encoding genes that are homologous to both ssRNA and ssDNA viruses. In order to gain a better understanding of this group of viruses both bioinformatic analyses and in vitro biochemical experiments were employed. The results of the bioinformatic analyses show that cruciviruses are a highly diverse group of ssDNA viruses. Their placement within established ssDNA phylogenies is difficult due to heterogeneity in their putative replication-associated …
Microbes On The Mountain: Plant-Microbe Associations And Interactions On Mount St. Helens, Emily Rose Wolfe
Microbes On The Mountain: Plant-Microbe Associations And Interactions On Mount St. Helens, Emily Rose Wolfe
Dissertations and Theses
Plant-microbe associations and interactions provide critical context to studies in both community and ecosystem ecology, especially in systems that are relatively new and still undergoing early successional processes. Microbes can colonize the surfaces and interiors of all plant tissues, and these assemblages vary in composition both spatially and temporally, even within the same plant. Endophytes are bacteria or fungi that spend most of their lifecycles living within plant tissues asymptomatically--typically, "endophyte" refers specifically to aboveground tissues such as leaves and stems, and therefore may have direct influences on defenses against herbivory, pathogen or pest tolerance, and even afterlife effects on …