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Full-Text Articles in Life Sciences

Biochemical And Proteomic Approaches To Determine The Impact Level Of Each Step Of The Supply Chain On Tomato Fruit Quality, Robert T. Madden Mar 2019

Biochemical And Proteomic Approaches To Determine The Impact Level Of Each Step Of The Supply Chain On Tomato Fruit Quality, Robert T. Madden

USF Tampa Graduate Theses and Dissertations

Fresh fruits and vegetables (FFVs) are the most frequently wasted foods because of their perishability and handling requirements. However, there is a lack of information on how much each step of the supply chain impacts FFVs quality, particularly on tomatoes, and what measures need to be taken for an immediate and effective impact on waste reduction. There is also no information on how the supply chain affects the proteome of the tomato and what proteins are differentially regulated by the most impactful steps of the supply chain. The objectives of the work presented on this thesis were to evaluate the …


Quantitative Proteome Analysis Of Alfalfa In Drought Stress Under The Influence Of Mir156, Alpa Puri Mar 2019

Quantitative Proteome Analysis Of Alfalfa In Drought Stress Under The Influence Of Mir156, Alpa Puri

Electronic Thesis and Dissertation Repository

Medicago sativa is one of the most produced perennial forage crops in Canada. Drought stress is a major form of abiotic stress affecting the productivity and annual yield of alfalfa. A small noncoding RNA, miR156 has a role in abiotic stress tolerance, while regulating downstream SPL genes. In this study, whole leaf proteome and amino acid concentrations of miR156OE (A8) genotype of alfalfa under drought stress were quantified. In results, 3,000 proteins groups were identified using MaxQuant when searched against Medicago truncatula protein sequence database. Gene ontology analysis showed enriched proteins were involved in biological and molecular processes such as …