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Full-Text Articles in Life Sciences
Metabolic Regulation And Cryotolerance Of In Vitro-Produced Holstein Embryos, Melissa Ann Roberts
Metabolic Regulation And Cryotolerance Of In Vitro-Produced Holstein Embryos, Melissa Ann Roberts
Master's Theses
In vitro production and transfer of embryos has become a common practice within the dairy industry to efficiently breed superior animals and meet the consumption demand of the growing population. Cyropreservation is necessary for the application of commercialized embryo transfer, however, in vitro-produced embryos show morphological and physiological defects which negatively impact their ability to withstand cryopreservation in comparison to their in vivo counterparts. These artifacts result from culture conditions that cause stress to the embryo during development, leading to an accumulation of intracellular lipids, mitochondrial dysfunction, and ultimately poor ability to withstand freezing and thawing. The objective of …
Vitrification Of Immature And Mature Bovine Oocytes, Paige T. Hardin
Vitrification Of Immature And Mature Bovine Oocytes, Paige T. Hardin
LSU Master's Theses
Vitrification is the latest technique used in cryopreservation, the ability to utilize this method with oocytes and embryos has become a valuable system. Vitrification has been successful with bovine embryos and oocytes but is far from optimal. Following cryopreservation storage discarding embryos can cause ethical issues, and mature oocytes have fragile organelles that can be detrimentally affected by ice crystal formation during freezing. Immature oocytes have not formed some of these temperature sensitive microfilaments and can circumvent these detrimental effects. The common intracellular cryoprotective agents are dimethyl sulfoxide, glycerol and ethylene glycol. Different combination of these agents have been reported …