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Full-Text Articles in Life Sciences
Modulation Of Cell-Matrix Interaction For Cryopreservation Of Engineered Tissue, Angela Christine Seawright
Modulation Of Cell-Matrix Interaction For Cryopreservation Of Engineered Tissue, Angela Christine Seawright
Open Access Theses
Long term preservation of functional engineered tissues can significantly advance tissue engineering industry and regenerative medicine. Several preservation techniques have been proposed and investigated for this purpose, and cryopreservation is a leading candidate. While tissues are cryopreserved, ice forms in both the extracellular and intracellular spaces and causes freezing-induced spatiotemporal deformation of the tissue. During this process the cells undergo dehydration by the freezing-induced osmotic pressure difference and mechanical deformation, transmitted through cell-extracellular matrix adhesions. However, the significance and interaction of these cellular level transport and mechanics processes are not well understood. Therefore, this study aims to establish mechanistic understanding …
Vitrification Of Equine Expanded Blastocysts, Fabian Andres Diaz
Vitrification Of Equine Expanded Blastocysts, Fabian Andres Diaz
LSU Master's Theses
The cryopreservation of equine expanded blastocysts (>300 um) has been largely unsuccessful primarily due to the low permeability to cryoprotectants and the large size of the equine embryo. Mechanical alternatives may provide means to overcome the capsule barrier and the relative large embryo size. In this regard, multiple experiments were performed in this study to evaluate different approaches of capsule puncture and blastocoele fluid extraction with the objective to develop a cryopreservation protocol for Day 8 equine expanded blastocysts. In the first experiment, twenty-four Day 8 expanded blastocysts were exposed to standard equine embryo vitrification solutions following one- or …