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Full-Text Articles in Life Sciences
A Fluorescent Photoinduced Electron Transfer (Pet) Sensor For Cations With A Separate Pet Channel To Suppress Proton Signals, Supun Pathirana
A Fluorescent Photoinduced Electron Transfer (Pet) Sensor For Cations With A Separate Pet Channel To Suppress Proton Signals, Supun Pathirana
Theses, Dissertations and Culminating Projects
This thesis presents A-((10-((2-methoxy-4-methylphenoxy)methyl)anthracen-9- yl)methyl)(pyridin-2-yl)-A-((pyridin-2-yl)methyl)methanamine, 1, a photoinduced electron transfer (PET) sensor that acts as a fluorescent ‘Off-On’ switch in the presence of Zn ions and not sensitive to protons.
Response Of The Salt Gland Of The Insectivorous Lizard Novoeumeces Schneideri To Ionic And Osmotic Challenges, Claudia Lechuga
Response Of The Salt Gland Of The Insectivorous Lizard Novoeumeces Schneideri To Ionic And Osmotic Challenges, Claudia Lechuga
Theses, Dissertations and Culminating Projects
The reptilian kidney can only produce isoosmotic waste. To supplement renal ion excretion and maintain ionic and osmoregulation, some reptile species rely on extrarenal salt glands that can secrete a hyperosmotic solution. Extrarenal glands among reptile taxa include the cranial gland in lizards, lacrimal gland of marine turtles, and sublingual gland of the estuarine crocodile and sea snakes. Secretion composition varies among these taxa, depending on diet, osmotic load and phylogeny. The salt glands of lizards are unique among reptiles in their ability to vary composition of the secreted fluid, secreting cations (potassium and/or sodium) and anions (chloride and/or bicarbonate). …
Optimization Of Expression And Purification Of Acetoacetyl-Coa Thiolase From Sunflower, Anthony Muriithi Maina
Optimization Of Expression And Purification Of Acetoacetyl-Coa Thiolase From Sunflower, Anthony Muriithi Maina
Theses, Dissertations and Culminating Projects
The [beta]-oxidation system in sunflower (Helianthus annuus L.) cotyledons is distinguished by the existence of two different thiolase isoforms, Thiolase I (acetoacetyl-CoA thiolase, EC 2.3.1.9 AACT) and Thiolase II (3-oxoacyl-CoA thiolase, EC 2.3.1.16 OACT). Glyoxysomal AACT is the last enzyme in the p-oxidation of fatty acids in plant glyoxysomes. Glyoxysomal AACT has been successfully cloned, expressed and purified from sunflower cotyledons (Dyer et al., 2006). In this paper we investigate the optimal conditions for both expression and purification. These include the choice of vector, the growth conditions and the purification parameters for the maximum possible yield of AACT protein.
Identification Of Metallo-Ss-Lactamase Inhibitors Via Enzyme Assay And Hplc-Ms Monitoring, Peggy Ann Sanchez
Identification Of Metallo-Ss-Lactamase Inhibitors Via Enzyme Assay And Hplc-Ms Monitoring, Peggy Ann Sanchez
Theses, Dissertations and Culminating Projects
The vast overuse of antibiotics along with extrachromosomal gene insertion between different bacterial species has led to the Darwinian consequence of antibiotic resistance. Amongst the contributing factors to the rise of bacterial resistances are the production of ß-lactamases, which pose a major impediment to treatment with ß-lactam drugs such as penicillins and carbapenems. SPM-1 is a metallo-ß-lactamase (MBL) that confers antibiotic resistance to bacteria through zinc-mediated hydrolysis of ß-lactams. Bacteria that express the SPM-1 metalloenzyme show reduced sensitivity to ß-lactam drugs. One method to defeat this form of resistance is to administer a ß-lactam antibiotic with a potent ß-lactamase inhibitor …
Evidence For Low Levels Of Genetic Variation Among Zostera Marina Populations From The Atlantic West Coast, Eric Rosenzweig
Evidence For Low Levels Of Genetic Variation Among Zostera Marina Populations From The Atlantic West Coast, Eric Rosenzweig
Theses, Dissertations and Culminating Projects
Zoster a marina (eelgrass), can be found in the North Atlantic on the coast of Europe and the North Arctic, as well as the Pacific. On the Atlantic west coast this species grows as far south as North Carolina and into northern Canada. The cold waters of these regions support the habitat needed for the species. Eelgrass is vital to coastal communities. It serves as a food source for many organisms, hatching ground for fish species, absorbent of water pollutants, and soil stabilizer. Over the last 30 years, the once robust populations of Atlantic west coast eelgrass have withered into …
Comparative Study Between Wild Rice (Oryza Rufipogon) And Domesticated Rice (Oryza Sativa Ssp Japonica) Chromosome 8 Centromere, Soham Aso
Theses, Dissertations and Culminating Projects
Rice has one of the simplest of the monocotyledonous genomes analyzed to date. The Oryza sativa genome is made of 12 chromosomes and has a total length of 430 MB. It is highly syntenic amongst other cereals. Therefore, genetic distances of these organisms are surprisingly very similar, meaning that genomic recombinations in each cereal occur at much the same frequency despite the differences in their genome size. The reason for this synchrony in recombinations may be due to similar lengths of gene-rich and gene-rare regions. This study focuses on the gene-rare repetitive centromeric region of rice chromosome 8. Although the …
Nutritional Evaluation Of Lean And Non-Lean Female High School Athletes Using Mypyramid, William Edward Mcsheffrey
Nutritional Evaluation Of Lean And Non-Lean Female High School Athletes Using Mypyramid, William Edward Mcsheffrey
Theses, Dissertations and Culminating Projects
MyPyramid was used to evaluate the nutritional status of twelve lean and fourteen non-lean high school female athletes. Lean athletes are those who participate in sports where weight control is considered important for performance. Weights of athletes participating in non-lean sports are less likely to influence performance. In this study, all the lean sport athletes participated in track, and the non-lean athletes competed in softball. Subjects included twenty-five middle-class Caucasians and one Hispanic from two northern New Jersey high schools. All subjects and their parents gave their consent to participate. Subjects needed to be in good health, between the ages …