Life Sciences Commons^™

Development And Characterization Of Monoclonal Antibodies Directed Against Tanapox Virus, Michael Franz

Masters Theses

The initial symptoms of a Tanapoxvirus (TPV) infection are indistinguishable from smallpox, which is caused by variola virus (VV). In the post-9/11 area, reagents that can quickly differentiate a TPV infection from smallpox are very desirable, but currently unavailable. Monoclonal antibodies (mAbs) directed specifically against TPV can be used for this purpose. Six mAbs were developed and characterized that specifically bind to TPV infected owl monkey kidney (OMK) cells, but not uninfected cells. The apparent molecular mass of the TPV protein(s) recognized by 5 mAbs were calculated using the Western blot technique. One mAb demonstrated no binding using the Western …

Mechanism Of Chromium Reduction Of Benzo[A]Pyrene Diolepoxide Mutagenesis In Human Fibroblasts, Cara Suzanne Kriss

Masters Theses

Environmental agents can cause an increase in cellular stress and damage DNA which can lead to diseases such as cancer. Chromium produces cellular stress by increasing the reactive oxygen species through a Fenton-like reaction, where chromium reacts with hydrogen peroxide to form hydroxyl radicals. Benzo[ a ]pyrene is an environmental carcinogen that is metabolized by cytochrome p450 enzymes into benzo[a]pyrene diolepoxide (BPDE). BPDE is highly reactive with DNA producing large DNA-adducts primarily on guanine nucleotides. The primary intracellular pathway by which mammalian cells remove damaged DNA caused by bulky mutagens is nucleotide excision repair. We have found that, in BPDE …

The Effects Of Elevated Co2 And Environmental Variation On Plant Phenotype, Herbivore Performance, And Evolutionary Potential In A Brassicacae Model System, John Michael Landosky

Dissertations

No abstract provided.

The Effects Of Differing Bicycle Frame Geometry On Muscle Recruitment Strategies And Power During A 30-Second Wingate Anaerobic Test, Patrick Hills-Meyer

Masters Theses

The purpose of this study was to test how changing the bicycle frame geometry; from a shallow frame geometry [seat tube angle (STA) of 72°] to a steep frame geometry (STA of 82°) affected the electromyographic (EMG) signal of the hamstrings [biceps femoris (BF) and semitendinosus (ST) muscles] and the quadriceps [vastus medialis (VM) and vastus lateralis (VL)] and power (max, mean, min, and, % drop) during a Wingate anaerobic test (WAT). To measure the muscle recruitment and power, 12 experienced triathletes were prepped with surface electrodes to measure EMG amplitude and an electrogoniometer to measure knee joint angle while …

Determining Differences In Quality Between The Gravure And Flexographic Process On Films, Pete Welther

Paper Engineering Senior Theses

Quality is the goal of every printing company. Every day printing companies spend money trying to find ways to improve the quality of their product. Type of paper, ink, and even the types of printing processes are all characteristics looked at when determining the quality of a printed product.

In today's printing industry, there are two printing processes that have arguably jumped to the front in terms of the highest quality printing ability. The flexographic process and the gravure process have both risen to meet the standards of high quality. The gravure process has always been at the top. For …

Strengthening Of Corrugated Boards By Chemical Impregnation, Rajesh Premnarayansingh

Masters Theses

Linerboards are used for making corrugated boards. The paperboards can be strengthened by adding strength additives at the wet-end of the paper machine and also by sizing them. Impregnation is another effective approach of strengthening the paperboards, mostly offline. The aim of the thesis is to evaluate the performance of the modified Dixon coater and check the operating conditions with various chemicals used for impregnating 42 lb/1000 sq.ft grammage linerboard.

The major conclusions of the research were that the edge compression strength increases markedly validating the usage of impregnation for linerboards. Also, the stiffness of linerboard increases and the voids …

Health Impacts Of Polychlorinated Biphenyls (Pcbs) In Aquatic Organisms, Marla Ann Fisher

Dissertations

Polychlorinated biphenyls (PCBs) are a major contaminant of global extent in water resources and aquatic biota. Due to its high lipid solubility, PCBs fail to be degraded and, therefore, continue to bioaccumulate throughout the environment and food chain. To determine the impact of PCBs on the immune system ofaged and juvenile Japanese medaka (Oryzias latipes), fish were injected with the coplanar PCB congener 126 and examined after 3 and 14 days. PCB 126 produced oxidative stress in both age groups of fish 14 days post-injection; however, juvenile medaka appeared more susceptible than aged fish. Humoral immunity, as determined by antibody …