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Full-Text Articles in Life Sciences
Effects Of Emphysema And Chronic Hypoxemia On Skeletal Muscle Oxygen Supply And Demand, John D. Lowman Jr.
Effects Of Emphysema And Chronic Hypoxemia On Skeletal Muscle Oxygen Supply And Demand, John D. Lowman Jr.
Theses and Dissertations
Skeletal muscle dysfunction in chronic obstructive pulmonary disease (COPD) is a condition in which peripheral skeletal muscle undergoes myopathic changes which impair muscle function, limit physical performance, and can lead to significant disability. While the etiology of the dysfunction is unknown, this study was conducted to test the hypothesis that chronic hypoxemia leads to alterations in oxygen transport and muscle function. A primary objective was to validate elastase-induced emphysema in rats as an animal model of skeletal muscle dysfunction in COPD.Arterial blood gases were used to determine the severity of hypoxemia and sodium dodecyl sulfate- polyacrylamide gel electrophoresis was used …
Surface Characteristics Of Bacillus Spores, Darlene Danette Sabio
Surface Characteristics Of Bacillus Spores, Darlene Danette Sabio
Theses and Dissertations
Rapid isolation and identification of spores from various environmental samples is necessitated because anthrax spores can be used as biological weapons. The hydrophobic nature of spores may allow for their rapid concentration and partial purification from contaminating materials. In this study, spores from four taxonomic groups of Bacillaceae were isolated, purified and characterized for hydrophobicity by hexadecane partitioning, surface morphology by scanning electron microscopy, and steady-state fluorescence by spectroscopy. The morphology of spores was similar within taxonomic groups and dissimilar between groups. Spore hydrophobicity ranged from 0.3% to 65% and all spores had fluorescence emission peaks at 335 nm and …
Regulation Of Fc Receptor Expression And Signaling On Murine Mast Cells, Hey Jin Chong
Regulation Of Fc Receptor Expression And Signaling On Murine Mast Cells, Hey Jin Chong
Theses and Dissertations
Mast cells have long been appreciated as the primary effector cells in allergy and asthma, and recently have been implicated in other inflammatory diseases. Using high density oligonucleotide probe arrays, we assessed genome-wide transcriptional profiles after FcεRI aggregation for 90 minutes, 5 hours and 24 hours. We describe novel gene regulation in response to FcεRI signaling, including altered expression of CD44, Pari, osteopontin, Nur77, E4BP4, and NDRG 1. In addition, the gene encoding FcεRI β was downregulated 5 hours after mast cell activation according to both microarray analysis and RPA, and western blotting confirmed the downregulation of the beta subunit …