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Articles 1 - 16 of 16
Full-Text Articles in Life Sciences
Cellular Immune Response And Gene Expression Profiling In Crohn's Dise, Claudia Romero
Cellular Immune Response And Gene Expression Profiling In Crohn's Dise, Claudia Romero
Electronic Theses and Dissertations
Despite the chronic debate in the etiology of crohn's disease (cd), a debilitating inflammatory bowel disease (ibd) closely related to ulcerative colitis (uc), an emerging interest in a possible mycobacterial role has been marked. Granuloma and pathologic manifestations in cd resemble aspects found in tuberculosis, leprosy and paratuberculosis. The latter, a chronic enteritis in cattle, goat, sheep and primates, which is similar to human enteritis, also known as cd, is caused by a fastidious, slow growing mycobacterium avium subspecies paratuberculosis (map). Due to the similarities between cd and paratuberculosis, a mycobacterial cause in cd has been proposed. Recent discovery of …
Characterization Of Protein Prenyltransferases And Protein Prenylation In Plasmodium Falciparum, Thiago Gaspar Dasilva
Characterization Of Protein Prenyltransferases And Protein Prenylation In Plasmodium Falciparum, Thiago Gaspar Dasilva
Electronic Theses and Dissertations
Malaria kills at least one million people each year, mostly children - a death every 30 seconds. Almost one half of the world population is at risk from malaria. Antimalarial drugs are the only means for the treatment of about 500 million annual global malaria cases. Because of prevalent drug-resistance it is extremely urgent to identify new drug targets. Many proteins involved in eukaryotic signal transduction and cell cycle progression undergo post-translational lipid modification by a prenyl group. Protein prenyltransferases, which catalyze the post-translational prenyl modification, have been established as a target for anticancer therapy. Research done in our laboratory …
Habitat Use And Seasonal Activity Of Selected Snakes On John F. Kenned, Karen Dyer
Habitat Use And Seasonal Activity Of Selected Snakes On John F. Kenned, Karen Dyer
Electronic Theses and Dissertations
An intensive, replicated monthly sampling of snake communities inhabiting four habitat types was conducted at John F. Kennedy Space Center, Brevard County, Florida from November 2002 through October 2003. Thirteen species (580 individuals plus 74 recaptures) plus one hybrid were captured. The three most commonly captured species, Coluber constrictor, Thamnophis sirtalis, and Thamnophis sauritus, combined made up 85% of the sample. These three species were active during every month of the year, but showed modal activity patterns typical of Temperate Zone snakes. Monthly snake captures were correlated with monthly captures of potential prey species and with mean monthly temperature. Species …
The Ecology Of Drift Algae In The Indian River Lagoon, Florida, Julie Lynnae Liss
The Ecology Of Drift Algae In The Indian River Lagoon, Florida, Julie Lynnae Liss
Electronic Theses and Dissertations
To gain an understanding of the ecology of drift algae in the Indian River Lagoon system along the east coast of central Florida, four questions were addressed: 1) What is the composition and rate of accumulation of drift? 2) How much movement and turnover occurs within drift accumulations? 3) Do growth rates differ for drift versus attached algae? 4) Is there a difference in photosynthetic performance in drift versus attached algal species? Manipulative field and laboratory experiments were conducted to address these questions with the green macroalga Codium decorticatum and the red macroalga Gracilaria tikvahiae. Changes in pigment concentration and …
Multigene Metabolic Engineering Via The Chloroplast Genome, Oscar Nemesio Ruiz
Multigene Metabolic Engineering Via The Chloroplast Genome, Oscar Nemesio Ruiz
Electronic Theses and Dissertations
The vast majority of valuable agronomic traits are encoded polygenetically. Chloroplast genetic engineering offers an alternate approach to multigene engineering by allowing the insertion of entire pathways in a single transformation event, while being an environmentally friendly approach. Stable integration into the chloroplast genome and transcription of the phaA gene coding for β-ketothiolase was confirmed by Southern and northern blots. Coomassie-stained gel and western blots confirmed hyperexpression of β-ketothiolase in leaves and anthers, with high enzyme activity. The transgenic lines were normal except for the male sterile phenotype, lacking pollen. Scanning electron microscopy revealed a collapsed morphology of the pollen …
Recruitment And Restoration Of The Oyster Crassostrea Virginica In Areas With Intense Boating Activity In Mosquito Lagoon, Florida, Lisa Michele Wall
Recruitment And Restoration Of The Oyster Crassostrea Virginica In Areas With Intense Boating Activity In Mosquito Lagoon, Florida, Lisa Michele Wall
Electronic Theses and Dissertations
Productivity, diversity and survival of estuaries are threatened by explosive coastal population growth and associated recreational activities. One major area of recreational growth has been the number of small pleasure craft motoring in shallow waters at high speeds. On the east coast of Central Florida in the Indian River Lagoon system, intense boating activity occurs year-round and intertidal reefs of the eastern oyster Crassostrea virginica with dead margins (piles of disarticulated shells) on their seaward edges are commonly found adjacent to major boating channels. The cause(s) of the dead margins is unclear. However, the disarticulated shells may be reducing reef …
Genetic Analysis Of Rhoa Signaling During Epithelial Morphogenesis In Drosophila, Amanda Fitch Leppert
Genetic Analysis Of Rhoa Signaling During Epithelial Morphogenesis In Drosophila, Amanda Fitch Leppert
Electronic Theses and Dissertations
Epithelial morphogenesis is contingent upon cell shape changes. Cell shape changes are the driving force for the metamorphosis of the adult Drosophila leg from the leg imaginal disc precursor. Genetic analysis has identified several Drosophila genes involved in regulating cell shape changes during leg disc morphogenesis. These include members of the RhoA signaling pathway and the product of the Stubble-stubbloid (Sb-sbd) locus, a transmembrane serine protease. Mutations in the Sb-sbd gene interact genetically with the members of the RhoA signaling pathway, however the nature of the relationship between Sb-sbd serine protease activity and RhoA signaling is not understood. To identify …
Extracullular Atp Regulates Il-1beta Release From Microglial Cells Via Purinergic Receptor After In Vitro Trauma, Chengya Liang
Extracullular Atp Regulates Il-1beta Release From Microglial Cells Via Purinergic Receptor After In Vitro Trauma, Chengya Liang
Electronic Theses and Dissertations
Traumatic brain injury (TBI) induces a state of microglialactivation, which includes upregulation of macrophage functions and release inflammatory mediators such as certain inflammatory cytokines. Current literature suggests that interleukin-1Beta is an important cytokine mediator, which is dramatically increased after brain injury. Previous studies indicate that ATP is released by traumatically injured astrocytes and serves as a cell-to-cell mediator through purinergic receptors after in vitro injury. However, the mechanism of interleukin-1Beta release after traumatic brain injury remains poorly defined and is difficult to study using in vivo models. Using an in vitro model for traumatic brain injury (cell strain or stretch), …
Land Use Effects On Lake Water Quality In Central Florida, Jonathan Seiler
Land Use Effects On Lake Water Quality In Central Florida, Jonathan Seiler
Electronic Theses and Dissertations
Land use affects the water quality of lakes. Different land use types yield different effects due to varying amounts and constituents of runoff. In this study, the effects of surrounding land use on the water quality of 50 lakes in Seminole County, Florida was assessed. Using GIS, I placed buffers of 100 and 500 m around each lake. The percentages of land use type were calculated within these buffers for 1990 and 1995. An ordination of lakes was done using Canonical Correspondence Analysis (CCA) to determine if the surrounding land use patterns were adequate to describe the trophic status of …
Possible Use Of P20 Antigen In Serodiagnosis Of Inflammatory Bowel Disease, Shinchieh Yang
Possible Use Of P20 Antigen In Serodiagnosis Of Inflammatory Bowel Disease, Shinchieh Yang
Electronic Theses and Dissertations
Crohn's disease (CD) is an idiopathic, chronic, relapsing inflammatory disorder, which is most commonly involved terminal ileum and colon. The incidence and prevalence of CD has dramatically increased during the last 50 years; however, the etiology and mechanism of this disorder remain unveiled. Besides genetic susceptibility, recent integrated researches investigated the role of environmental triggers such as microflora, measles viruses and mycobacteria in the pathogenesis of CD. The association between M. avium subsp paratuberculosis (MAP) and CD has been heightened because of clinical resemblance to Johne's disease (JD), a granulomatous enteritis in ruminants caused by MAP. Isolation of MAP from …
Evaluation Of Immunogenicity Of Transgenic Chloroplast Derived Protect, Vijay Koya
Evaluation Of Immunogenicity Of Transgenic Chloroplast Derived Protect, Vijay Koya
Electronic Theses and Dissertations
Anthrax, a fatal bacterial infection is caused by Bacillus anthracis, a gram-positive, spore forming, capsulated, rod shaped organism. Centers for Disease Control (CDC) lists anthrax as Category A biological agent due to its severity of impact on human health, high mortality rate, acuteness of the disease and potential for delivery as a biological weapon. The currently available human vaccine in the United States (AVA anthrax vaccine adsorbed) is prepared from Alum adsorbed formalin treated supernatant culture of toxigenic, non-encapsulated strain of Bacillus anthracis with the principle component being protective antigen (PA83). Evaluation of anthrax vaccine given to nearly 400,000 US …
Interactions Between The Nudibranch Okenia Zoobotryon And Its Bryozoan, Nicole Robinson
Interactions Between The Nudibranch Okenia Zoobotryon And Its Bryozoan, Nicole Robinson
Electronic Theses and Dissertations
In the Indian River Lagoon, the nudibranch Okenia zoobotryon and its egg masses are found attached to the bryozoan Zoobotryon verticillatum throughout the year. Okenia zoobotryon is thought to live, feed, and reproduce exclusively on Z. verticillatum, which would make this a very specialized predator-prey interaction. The primary goal of my study was to document certain aspects of the ecological and chemical relationships between Z. verticillatum and O. zoobotryon. Specifically, I wanted to understand the cues used by the nudibranch to locate and remain on Z. verticillatum. Population surveys on Z. verticillatum, as well as other potential hosts, were performed. …
Survival Of Mycobacterium Avium Subspecies Paratuberculosis In The Pol, John Rumsey
Survival Of Mycobacterium Avium Subspecies Paratuberculosis In The Pol, John Rumsey
Electronic Theses and Dissertations
Mycobacterium avium subspecies paratuberculosis (map) is an intracellular pathogen that is known to parasitize macrophages and monocytes. Map infiltrates gastrointestinal tract host tissue where it is the known etiological agent of johne's disease in ruminants and implicated in the etiology of crohn's disease in humans. Map's ability to survive within macrophages enables it to disseminate throughout the rest of the host, possibly infecting other circulating blood leukocytes. In this study, the survival and fate of map strain atcc 43015 (human isolate) following phagocytosis was determined using in vitro murine macrophage cell line j774a.1 and polymorphonuclear cells (pmnc's) from five crohn's …
Characterization Of A Novel Interactor/Substrate For The Pro-Apoptotic Serine Protease Omi/Htra2, Valerie Anne Stratico
Characterization Of A Novel Interactor/Substrate For The Pro-Apoptotic Serine Protease Omi/Htra2, Valerie Anne Stratico
Electronic Theses and Dissertations
OmiHtrA2 is a highly conserved mammalian serine protease that belongs to the HtrA family of proteins. Omi shares homology with the bacterially expressed heat shock protease HtrA, which functions as a protease at higher temperatures and a chaperone at lower temperatures. Additionally, Omi shares sequence similarity with the mammalian homologs L56/HtrA1 and PRSP/HtrA3. Omi was first isolated as an interacting protein of Mxi2, an alternatively spliced form of the p38 stress-activated kinase, using a modified yeast two-hybrid system. Omi localizes in the mitochondria and in response to apoptotic stimuli the mature form of this protein translocates to the cytoplasm. In …
Molecular Typing Of Mycobacterial Isolates Cultured From The Tissue Of Inflammatory Bowel Disease (Crohn's Disease) Patients, Leanne M. Adams
Molecular Typing Of Mycobacterial Isolates Cultured From The Tissue Of Inflammatory Bowel Disease (Crohn's Disease) Patients, Leanne M. Adams
Electronic Theses and Dissertations
The role of Mycobacterium avium subsp paratuberculosis (MAP) in the etiology and pathogenesis of inflammatory bowel disease (IBD) including Crohn's Disease (CD), has been investigated. The fastidious characteristics and cross reactivity of MAP with other members in Mycobacteria have produced significant challenges in their detection and identification. In this two year pilot study, an array of three PCR molecular assays based on the detection of sequences from the16S rRNA, IS1245, and IS900 genes, belonging to members of the MAC, have been developed and optimized into a common protocol to be used as a rapid and accurate diagnostic tool regarding M. …
Plasma Levels Of Vitamins A And E In Marine Turtles (Chelonia Mydas And Caretta Caretta), Karen Patricia Frutchey
Plasma Levels Of Vitamins A And E In Marine Turtles (Chelonia Mydas And Caretta Caretta), Karen Patricia Frutchey
Retrospective Theses and Dissertations
Insight into blood values for free-ranging populations of chelonians is very incomplete. A better understanding of marine turtle ecological physiology is necessary for population health assessments and conservation management plans. Vitamins A and E are fat-soluble compounds required by vertebrates. Vitamin A is required for growth, differentiation and integrity of epithelial tissue, bone remodeling, reproduction and vision. Vitamin E is a constituent of cell membranes where it is an antioxidant and free radical scavenger. Both nutrients are required for reproduction and immune system function. I determined concentrations of vitamins A and E in plasma of blood samples from Caretta caretta …