Life Sciences Commons^™

Methods For Detection Of Salmonella Spp., Parichaya Attaphongse

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Worldwide concern about food safety and associated health costs is increasing, and Salmonella contamination of foods is one of the most important causes of foodborne disease outbreaks. Although conventional detection methods for Salmonella offer high sensitivity and low cost, they require many different steps and are very time consuming. Ideally, methods to detect Salmonella in foods should be rapid, provide high specificity and sensitivity, be cost-effective, and low labor-intensive. Many rapid tests have been developed to address these goals, including DNA-based tests such as nucleic acid hybridization and PCR, immunoassay-based tests such as ELISA, ELF A and immunomagnetic method, and …

Development And Characterization Of Eukaryotic Biomimetic Liposomes, Bradley Jay Taylor

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study developed and characterized phospholipid vesicles, or liposomes, that mimic cell surfaces. Microemulsified liposomes contained biotinylated phosphatidylethanolamine, allowing them to be immobilized to avidin-coated glass. Laminin (LN), glycosphingolipids (GMl and GM3), and Escherichia coli's mechanosensitive channel of large conductance (EcoMscL) were embedded into liposome membranes. It was determined whether these embedded molecules exhibited their physiological roles of adhesion, cell recognition, and mechanosensation, respectively. Confocal laser scanning microscopy (CLSM) was employed to examine the interaction of fluorescently probed proteins, toxins, and bacteria with the immobilized microemulsified liposomes. Capture of individual and simultaneous multiple species of bacteria by GMl, GM3, …

Hormone Mediated Transport Of Calcium And Phosphate In Polarized Epithelial Cells, Tremaine M. Sterling

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effects of 1,25(OH)₂D₃, PTH and 25(OH)D₃ on phosphate or calcium uptake were studied in cultured, adherent chick enterocytes over a period of 10 min after hormone addition. Time course studies of cells treated with 130 pM 1,25(OH)₂D₃ showed an increase in ³²P uptake as early as 3 min. Similar studies with 65 pM bPTH(l1-34) resulted in an increase in ⁴⁵Ca uptake only if the cells had been cultured in serum. (OH)D₃, which is not firmly established as an active metabolite of vitamin D, was shown to increase …

Rapid Detection Of Salmonella Without Enrichment, Emily J. Harrington

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Salmonella is one of the leading foodborne pathogens causing illness today. Because of this, Salmonella rapid detection methods are under immense study for use in food. The traditional method, using the Food and Drug Administration- approved Bacterial Analytical Manual procedure, takes 4-6 days for Salmonella detection in food. Other rapid methods still take at least 16 h for detection due to their enrichment steps.

The hypothesis of this study was that the use of immobilized antibodies coupled with polymerase chain reaction (PCR) can be used for the rapid capture and detection of Salmonella spp. in food without the need for …