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Laser-Assisted Zona Pellucida Hatching Of Frozen-Thawed In Vivo-Produced Bovine Embryos, Mindy Kaye Chiasson
Laser-Assisted Zona Pellucida Hatching Of Frozen-Thawed In Vivo-Produced Bovine Embryos, Mindy Kaye Chiasson
LSU Master's Theses
Incomplete zona hatching or failure of the zona to rupture compromises post-transfer embryo viability and conceptus development. Assisted hatching prior to the transfer of frozen-thawed bovine embryos has been proposed as a means to increase recipient pregnancy rates. The objective of this study was to determine if laser-assisted hatching would improve in vivo-produced frozen-thawed bovine embryo hatching and pregnancy rates. In Experiment I,II and III frozen direct-transfer embryos received either two a or three symmetrical rents at either 40% or 80% through the outer zona surface using the Hamilton Thorne XYClone® (Hamilton Thorne Biosciences) diode laser at 90% power with …