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Full-Text Articles in Life Sciences
Development Of Functional Markers For Resistance To Smut And Identification Of Genes Differentially Expressed In Response To Brown Rust In Sugarcane, Jose David Cortes
Development Of Functional Markers For Resistance To Smut And Identification Of Genes Differentially Expressed In Response To Brown Rust In Sugarcane, Jose David Cortes
LSU Doctoral Dissertations
Smut, caused by Sporisorium scitamineum and brown rust, caused by Puccinia melanocephala, are two of the most important diseases that affect sugarcane production in Louisiana and worldwide. Smut continues to cause losses in the breeding program in Louisiana. Therefore, a QTL analysis was conducted to identify molecular markers associated with resistance in F1 progeny (162) of a biparental cross between susceptible cultivar L 99-233 and resistant HoCP 96-540. Using 1,574 single-dose SNP markers, a total of 253 linkage groups (LG) were obtained with a genome coverage of 24,580.15 cM. Six QTLs were localized on five LGs. The highest …
Integrated Nutrient Management And Cover Cropping Practices In Louisiana Sugarcane Production Systems, Daniel Erneso Forestieri
Integrated Nutrient Management And Cover Cropping Practices In Louisiana Sugarcane Production Systems, Daniel Erneso Forestieri
LSU Doctoral Dissertations
Integrating effective fertilizer management with conservation practices is essential to improve farm income and promote environment friendly sugarcane production systems. A study was conducted in multiple to document the impact of nitrogen (N) source and cover cropping on sugarcane yield and quality components. The treatment consisted of different N sources: urea, knife-in urea ammonium nitrate (UAN) solution, calcium nitrate-CaNO3, ammonium sulfate-NH4SO4, and knife-in UAN. + foliar N, applied at 90 kg ha-1 and control (0 N). The cover crops included: crimson clover (Trifolium incarnatum), hairy vetch (Vicia villosa), and oilseed radish (Raphanus sativus L.) planted at 13, 17, and 1 …
Genome Wide Association Studies For Identification Of Markers Linked To Sucrose Traits In Sugarcane, Nathanael Darrell Fickett
Genome Wide Association Studies For Identification Of Markers Linked To Sucrose Traits In Sugarcane, Nathanael Darrell Fickett
LSU Doctoral Dissertations
Sugarcane, being a complex aneu-polyploid, poses unique challenges to fine mapping of quantitative trait loci (QTL) controlling agronomic traits of interest. Genome-wide association studies (GWAS) could be a better alternative to identify molecular markers associated with specific traits due to high linkage disequilibrium in sugarcane. In the first study, marker-trait associations (MTAs) were evaluated for three sucrose traits, Brix, total recoverable sugar (TRS), and percent sucrose on 48 elite and historic Louisiana breeding clones using 1,062 alleles. These sucrose traits were highly correlated (P-value < 0.0001) at >0.96. TASSEL 5.1 and JMP Genomics 8.0 were compared with eight models run in each program. …
Identification Of Genes Associated With Resistance To Brown Rust In Sugarcane And Prevalence Of One Major Gene, Mavir Carolina Avellaneda Barbosa
Identification Of Genes Associated With Resistance To Brown Rust In Sugarcane And Prevalence Of One Major Gene, Mavir Carolina Avellaneda Barbosa
LSU Doctoral Dissertations
Development of resistant cultivars is the main control measure against sugarcane brown rust caused by Puccinia melanocephala. Durability is uncertain, since the pathogen possesses adaptive ability to overcome host plant resistance. A differential gene expression study utilizing suppressive subtraction hybridization was conducted to improve understanding of brown rust resistance mechanisms in sugarcane. The expression patterns of 11 unigenes representing biosynthetic pathways, defense-related genes, and signaling genes were analyzed in L 99-233, a cultivar exhibiting quantitative resistance, L 01-299, a resistant cultivar with the major resistance gene Bru1, and two susceptible cultivars, Ho 95-988 and L 09-125, at 24 h, 48 …
Development Of Functional Markers For Resistance To Leaf Scald In Sugarcane, Andres Felipe Gutierrez Viveros
Development Of Functional Markers For Resistance To Leaf Scald In Sugarcane, Andres Felipe Gutierrez Viveros
LSU Doctoral Dissertations
Leaf scald, caused by Xanthomonas albilineans, is a major sugarcane disease worldwide. The disease is managed primarily with resistant cultivars obtained through classical breeding; however, the erratic symptom expression hinders the reliability and reproducibility of the selection process. The development of molecular markers associated with incompatible/compatible reaction can overcome this limitation. Suppression subtractive hybridization (SSH) and quantitative trait locus (QTL) mapping were the strategies used to find leaf scald resistance-associated genes and molecular markers in sugarcane. SSH results showed that genes involved in signal perception and transduction, and DNA binding, were highly expressed in the resistant clone LCP 85-384 compared …
Mexican Rice Borer (Eoreuma Loftini) Pheromone Trap Efficacy And Role In Invasive Species Monitoring And Pest Management, Blake Emerson Wilson
Mexican Rice Borer (Eoreuma Loftini) Pheromone Trap Efficacy And Role In Invasive Species Monitoring And Pest Management, Blake Emerson Wilson
LSU Doctoral Dissertations
The Mexican rice borer, Eoreuma loftini (Dyar) (Lepidoptera: Crambidae), is an invasive pest of sugarcane, Saccharum spp.; rice, Oryza sativa; and other graminaceous crops along the U.S. Gulf Coast. Identification of E. loftini sex pheromones led to the development of pheromone baited traps. Studies were conducted to determine strategies for the use of E. loftini pheromone traps in invasive species monitoring and pest management. A two-year field study demonstrated that E. loftini pheromone traps attract males from distances of up to 100m. A behavioral assay observed that detection of the pheromone by E. loftini males occurs at ≈48m from the …
The Economics Of Processing Ethanol At Louisiana Sugar Mills: A Three Part Economic Analysis Of Feedstocks, Risk, Business Strategies, And Uncertainty, Paul Michael Darby
The Economics Of Processing Ethanol At Louisiana Sugar Mills: A Three Part Economic Analysis Of Feedstocks, Risk, Business Strategies, And Uncertainty, Paul Michael Darby
LSU Doctoral Dissertations
The development of an efficient processing infrastructure is critical for the budding cellulosic ethanol industry. Developing a diverse feedstock portfolio is one crucial part of this process which can lead towards economically feasible cellulosic ethanol production. Cellulosic ethanol production requires the production and transportation of large quantities of biomass. Sugarcane and other dense grasses offer a compelling path towards a successful biomass supply chain. The Louisiana sugar belt already has an infrastructure adapted to the task of biomass supply, and taking advantage of this is one key way that a cellulosic ethanol plant can benefit from the region’s endowment. Additionally, …
Comparative Proteome And Qpcr Analysis Of The Sugarcane Reaction To Leaf Scald Caused By Xanthomonas Albilineans, Freddy Fernando Garces-Obando
Comparative Proteome And Qpcr Analysis Of The Sugarcane Reaction To Leaf Scald Caused By Xanthomonas Albilineans, Freddy Fernando Garces-Obando
LSU Doctoral Dissertations
Leaf scald is an important disease of sugarcane caused by Xanthomonas albilineans (Xa). Leaf scald is controlled by the development and planting of resistant cultivars. However, erratic symptom expression makes conventional screening for resistance difficult. In addition, the mechanisms of resistance to leaf scald are not completely understood. Real-time, quantitative polymerase-chain-reaction (qPCR) assays were developed utilizing SYBR Green for a highly sensitive detection method or a TaqMan probe to quantify Xa populations in infected plants. Xa populations detected by qPCR followed similar trends to disease severity ratings and vascular infection results for two resistant and two susceptible cultivars under greenhouse …