Life Sciences Commons^™

Evaluation Of Soil Type And Seeding Rate On Winter Cover Crop Species In A Soybean Production System, Donna S. Morgan

LSU Doctoral Dissertations

The integration of winter annual cover crops into a cropping system can potentially improve soil health and crop production, however, the impact of variables such as seeding rates, across two very different soil types, has not been well documented. A two-year study was conducted at the Dean Lee Research Station and Extension Center in Alexandria, Louisiana to evaluate the effects of cover crop seeding rate and soil type on cover crop biomass, weed suppression, soil fertility, and soybean (Glycine max L) growth and yield. Analysis of potential economic impacts was also performed to estimate financial net returns for three …

Transgenic Control Of Aflatoxin Contamination In Maize Through Host-Induced Gene Silencing Targeting Aspergillus Flavus Genes Encoding Polygalacturonase (P2c) And Versicolorin Dehydrogenase (Aflm), Yenjit Raruang

LSU Doctoral Dissertations

Maize (Zea mays L.) is susceptible to Aspergillus flavus infection and subsequent contamination with aflatoxins, the most potent naturally produced carcinogenic secondary metabolites. Here, the A. flavus gene aflM encoding the versicolorin dehydrogenase in aflatoxin biosynthesis and the p2c gene encoding the polygalacturonase that is involved in infection were selected as targets for suppression through host induced gene silencing (HIGS). A HIGS vector targeting these genes was constructed and introduced into immature B104 maize embryos. Thirteen out of fifteen p2c transformation events and six out of seven aflM events were confirmed positive by PCR. Kernels containing the p2c gene …

Using Poultry Litter Ash As A Fertilizer Source For Bermudagrass (Cynodon Dactylon) Establishment And Loblolly Pine (Pinus Taeda) Plantation, Crystal L. Vance

LSU Doctoral Dissertations

Lower solubility of P in poultry litter ash (PLA) than conventional phosphorus (P) fertilizers such as triple superphosphate (TSP) may reduce P losses in drainage and runoff; however, lower P solubility may reduce its efficacy as a P source. A laboratory experiment was conducted to examine the effect of acidity on P release and the effect of different combustion temperatures on P solubility as a prerequisite to the following studies. The first research objective was to determine the effect of soil pH on the efficiency of PLA relative to TSP for establishment of common bermudagrass (Cynodon dactylon L.) and …

Identification Of Quantitative Trait Loci (Qtls) For Resistance To Fusarium Head Blight (Fhb) In Wheat Variety Ags 2060 And Evaluation Of The Effect Of Fhb1 Resistance Gene On Fhb Reaction In A Louisiana Wheat Breeding Population, Alejandro Castro Aviles

LSU Doctoral Dissertations

Fusarium head blight (FHB), caused by the fungus Fusarium spp., is a disease that attacks several grass species, including wheat. Wheat variety AGS 2060 has remained moderately susceptible to FHB and is regularly found in pedigrees of resistant breeding lines. Quantitative trait loci (QTL) mapping was conducted to find sources of resistance to FHB in AGS 2060. A 90K SNP chip was used to genotype a double haploid mapping population produced from a cross between wheat varieties AGS 2060 and AGS 2035. There were highly significant differences between lines in the population for Fusarium damaged kernels (FDK) rating and deoxynivalenol …

The Role Of Chromatin State Transitions In Modulating Early Phosphate-Deficiency Response Genes In Plants, Maryam Foroozani

LSU Doctoral Dissertations

Phosphorus (P) is an essential plant macronutrient vital to fundamental metabolic processes. Plant-available P is low in most soils, making it a frequent limiter of growth. Declining P reserves for fertilizer production exasperates this agricultural challenge. Plants modulate complex responses to fluctuating P levels via global transcriptional regulatory networks. Although chromatin structure plays a substantial role in controlling gene expression, the chromatin-level mechanisms involved in regulating P homeostasis have not been determined.

In this work, I used chromatin immunoprecipitation (ChIP) combined with next-generation DNA sequencing (ChIP-seq) to map the distribution of H3K4me3 in rice (Oryza sativa L.) during both …

Silicon Fertilization In Rice And Wheat: Dynamics With Trace Elements And Effect Of Silicate Slag Granular Size On The Release Pattern Of Monosilicic Acid In Soil, Wooiklee S. Paye

LSU Doctoral Dissertations

Studies were conducted to evaluate the impact of silicon (Si) fertilization on the uptake of harmful trace elements in Louisiana rice production system, and document the effect of silicate slag granular size on its ability to supply Si in wheat. The influence of silicate slag and lime on the uptake and translocation of harmful trace elements in rice grain was determined. Results showed that both slag and lime had similar effects on pH at five of the nine sites investigated. Soil Si was significantly (P <0.001) increased with application of silicate slag at all sites. Lime and silicate slag had no effect on the concentration of trace elements in soil. However, increasing Si rates were negatively correlated with arsenic (As) and cadmium (Cd) concentration in rice grain. The release pattern of monosilicic acid (H₄SiO₄) from wollastonite and silicate slag was compared in a wheat greenhouse …

Characterization Of The Integrated Signaling Network Of Burkholderia Glumae For The Regulation Of Virulence-Related Function In The Bacterial Pathogenesis Of Rice Plants, Tiago De Paula Lelis

LSU Doctoral Dissertations

Bacterial panicle blight of rice (BPB) caused by Burkholderia glumaeis a major bacterial disease of rice in many rice-producing areas worldwide. Managing the disease is still very difficult due to the lack of effective methods. B. glumaestrains show phenotypic and genomic variations; however, little variation was detected in the known virulence-related genes. In the present study, the complete genome of B. glumae257sh-1, a natural avirulent strain, was sequenced and analyzed. Several spontaneous mutations in the regulatory genes of these avirulent strains were identified. Presumably, the accumulation of these mutations can have a negative effect on the normal …

Improving The Food Safety Of Louisiana Strawberry Industry, Jose L. Brandao Delgado

LSU Doctoral Dissertations

Escherichia coli O157:H7, Salmonella sp., and Listeria monocytogenes have been linked to foodborne outbreaks in produce. The most recent outbreaks in produce have been associated with irrigation water due to infiltration of well water or water run-off from contaminated sources. The Food Safety Modernization Act (FSMA) requires all irrigation water to be safe for use on produce, as a strategy to reduce foodborne illnesses. A surfactant modified zeolite (SMZ) filtration system could provide produce farmers with a sustainable low-cost system for high-quality and safe irrigation water. The objective of this study was to evaluate the effectiveness of hexadecyltrimethylammonium …

The Tripartite Interaction Between Arbuscular Mycorrhizal Fungi, Rice, And Insects, Lina Bernaola Alvarado

LSU Doctoral Dissertations

Losses caused by pests remain an important limitation to achieving high rice yields in the United States. Arbuscular mycorrhizal fungi (AM fungi) are able to modify plant physiology by increasing plant growth or inducing defense responses against insect herbivores. However, studies of the role of AM fungi in agroecological factors, including natural occurrence, plant resistance, soil dependency, and plant tolerance, with specific regards to pests that feed on rice plants have not been conducted before. A three-year study revealed natural occurring colonization by AM fungi on rice roots sampled in four rice-producing areas in the southern United States. Overall, rice-AM …

Development Of Sheath Blight-Resistant Breeding Lines For Southern U.S. Environments And Morphological And Genetic Survey Of Giant Salvinia Populations In Louisiana And Texas, Dominique Clark Galam

LSU Doctoral Dissertations

The southern US environment is a very conducive environment for agriculture and fisheries. Rice farming, shrimping and water related activities help drive the local economy. However, there are several factors that impede the success of these activities. Sheath blight (SB) disease caused by Rhizoctonia solani is one of the major biotic constraints to high grain yield and quality for most commercial U.S. rice varieties. Although different breeding lines with high levels of "partial resistance" have been developed none has been used directly as a commercial variety. The first research objective of this research was to identify and develop advanced breeding …

Genetic Analysis Of Drought Tolerance In Rice (Oryza Sativa L.), Uttam Bhattarai

LSU Doctoral Dissertations

Drought is a major challenge in rice production system worldwide. We conducted a phenotypic screening of the USA rice genotypes for drought tolerance and assessed genetic diversity using SSR markers. Identification of QTLs for drought tolerance during both vegetative and reproductive stage was done using genotyping by sequencing (GBS) based saturated SNP linkage map. The rice genotypes Jes, Leah, Roy J, Jazzman, and Madison were found to be drought tolerant. Population structure analysis grouped the USA rice genotypes into California, Louisiana, and Arkansas types. Marker-trait association showed that markers RM570 and RM351 were significantly associated with grain yield, spikelet fertility, …

Investigations Into Aspergillus Flavus Infection Of Corn And Regulation Of Aflatoxin Production By Volatiles And Biocontrol Strains, Rebecca R. Sweany

LSU Doctoral Dissertations

Aspergillus flavus is an ascomycete fungus impacting agriculture, public and environmental health due to production of acutely-toxic, carcinogenic aflatoxins in oil seed crops, especially corn in Louisiana, and lung infections of immunocompromised patients resulting in a leading cause of AIDS patients’ deaths globally. The most effective aflatoxin mitigation strategy, biocontrol by atoxigenic A. flavus presents several concerns including: dissemination of a human pathogen, possible sexual reproduction and reliance on precise environmental conditions. Several aspects of A. flavus biology were investigated to potentially improve treatments. Studies investigated how genotypically diverse members within the A. flavus population interact with other population members …