Life Sciences Commons^™

Examining Intracellular Phosphorylation Gradients During Cell Division, Lei Tan

Student Theses and Dissertations

The dynamic cellular reorganization needed for successful mitosis requires spatial regulatory cues. I examine this problem at two different levels. First, I analyze a phosphorylation gradient for substrates of the chromosomal passenger complex (CPC) . CPC is a conserved regulator involved in key mitotic events such as chromosome-microtubule attachment and spindle midzone formation. Previously, spatial phosphorylation gradients have been reported for CPC substrates, raising the possibility that CPC-dependent signaling establishes order on the micron-length scale in dividing cells. However, this hypothesis has not been tested, largely because of incomplete characterization of the CPC-dependent phosphorylation dynamics. Here I examine the spatiotemporal …