Life Sciences Commons^™

Bioorthogonal Chemical Reporters Reveal Fatty-Acylation Of Histone H3 Variants And Cholesterol Modification Of Proteins And Trafficking In Cells, John P. Wilson

Student Theses and Dissertations

Lipids are essential components to all known life. They serve many functions from basic building blocks to covalent protein modification and are particularly involved in signaling. Many rare and widespread human health concerns involve lipids but despite their importance, their analysis either free or protein-bound has remained difficult for decades. Here, using bioorthogonal chemistry, we have developed a robust system to identify the proteins modified by lipids and advanced the ability to image cholesterol in cells and tissues. The unbiased proteomic analysis of fatty-acylated proteins using chemical reporters undertaken here revealed a greater diversity of lipid-modified proteins in mammalian cells …