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The Effects Of Solutes In The Cryopreservation Of Adherent Neuroblastoma (Neuro-2a) Cells, Trisha L. Bailey
The Effects Of Solutes In The Cryopreservation Of Adherent Neuroblastoma (Neuro-2a) Cells, Trisha L. Bailey
Masters Theses
A simple method to cryopreserve adherent monolayers of neuronal cells is currently not available, but the development of this technique could facilitate numerous applications in the field of biomedical engineering, cell line development, and drug screening. However, complex tissues of some exceptional animals survive freezing in nature. These animals are known to accumulate several small molecular weight solutes prior to freezing. Following a similar strategy, we investigated the effects of osmolytes such as trehalose, proline, and sucrose as additives to the traditional cryoprotectant dimethyl sulfoxide (Me2SO) in modulating the cryopreservation outcome of mouse neuroblastoma (Neuro-2a) cells. Neuro-2a cells adhered to …