Life Sciences Commons^™

Culturally Speaking: You Don't Know Beans About Cottage Cheese, Phillip Tong

Dairy Science

No abstract provided.

Phylogeography Of The California Sheephead, Semicossyphus Pulcher: The Role Of Deep Reefs As Stepping Stones And Pathways To Antitropicality, Marloes Poortvliet, Gary C. Longo, Kimberly Selkoe, Paul H. Berber, Crow White, Jennifer E. Caselle, Alejandro Perez-Matus, Steven D. Gaines, Giacomo Bernardi

Biological Sciences

In the past decade, the study of dispersal of marine organisms has shifted from focusing predominantly on the larval stage to a recent interest in adult movement. Antitropical distributions provide a unique system to assess vagility and dispersal. In this study, we have focused on an antitropical wrasse genus, Semicossyphus, which includes the California sheephead, S. pulcher, and Darwin's sheephead, S. darwini. Using a phylogenetic approach based on mitochondrial and nuclear markers, and a population genetic approach based on mitochondrial control region sequences and 10 microsatellite loci, we compared the phylogenetic relationships of these two species, as well …

Luxs In Bacteria Isolated From 25- To 40-Million-Year-Old Amber, Tasha M. Santiago-Rodriguez, Ana R. Patrício, Jessica I. Rivera, Mariel Coradin, Alfredo Gonzalez, Gabriela Tirado, Raúl J. Cano, Gary A. Toranzos

Biological Sciences

Interspecies bacterial communication is mediated by autoinducer-2, whose synthesis depends on luxS. Due to the apparent universality of luxS (present in more than 40 bacterial species), it may have an ancient origin; however, no direct evidence is currently available. We amplified luxS in bacteria isolated from 25- to 40-million-year-old amber. The phylogenies and molecular clocks of luxS and the 16S rRNA gene from ancient and extant bacteria were determined as well. Luminescence assays using Vibrio harveyi BB170 aimed to determine the activity of luxS. While the phylogeny of luxS was very similar to that of extant Bacillus spp., …

Rov-Based Tracking Of A Shallow Water Nocturnal Squid, Samuel Yim, Christopher M. Clark, Timothy Peters, Vladimir Prodanov, Pat Fidopiastis

Biological Sciences

This paper describes the use of a Remotely Operated Vehicle (ROV) equipped with a monocular vision system to find and track the squid Euprymna scolopes, so that motion behaviors of the squid could be characterized through the use of off-line image processing and state estimation. The ROV was deployed for several nights at several nearshore locations off Oahu, resulting in 10 hours of squid footage. Using blob-tracking image processing techniques and a Particle Filter state estimator, the squid can be detected and tracked. The position, velocity, and acceleration of the squid relative to the stationary ROV can be determined. Experiment …

School Gardens, David W. Hey

Kinesiology and Public Health

School gardens can have great promise as they can positively impact children's food choices by improving their preferences for vegetables, increasing their nutrition knowledge, and prevent nutritionally related diseases (i.e., diabetes and obesity). In their natural state, fruits and vegetables have high water and fiber content and are low in calories and energy density. Additionally, it has been demonstrated that the use of a garden as part of an outdoor environmental studies program can have a beneficial impact on performance of standardized achievement tests, as well as attention and enthusiasm for learning in school children.

In June of 2004, national …

Culturally Speaking: Lactose Intolerance: A Condition As Old As The Stone Age, Phillip Tong

Dairy Science

No abstract provided.

Promoting Weight Loss Before Pregnancy: Feasible Or Futile?, Suzanne Phelan, Todd Hagobian, Anna Brannen, Ana Stewart, Brianna Schmid, Leonard Cedars

Kinesiology and Public Health

Pre-pregnancy obesity is a well-established risk factor for several adverse maternal and fetal outcomes, including gestational diabetes, hypertension, cesarean sections, and fetal macrosomia. Weight loss before pregnancy could help prevent such complications, but the feasibility of such an approach remains unknown. The current study examined the feasibility of a 3-month pre-pregnancy behavioral weight loss program in 12 overweight/obese women planning pregnancy. The 3 month program resulted in an average 5.4 ± 3.0 kg weight loss and significant improvements in self-monitoring, physical activity, eating and exercise self-efficacy, and healthy eating (p < 0.04). By the end of the 9 month follow-up, half of sample (n = 6) had conceived. Women reported significant increases in weekly or more frequent self-weighing (p < 0.0001), counting calories (p < 0.001), consuming fruit and vegetables (p = 0.007), and cutting out fat (p = 0.0001) and junk foods (p = 0.002). A lifestyle modification program to promote weight loss before pregnancy promoted clinically significant weight loss and appeared feasible.

Evaluation Of Species Distribution Models By Resampling Of Sites Surveyed A Century Ago By Joseph Grinnell, Adam B. Smith, Maria J. Santos, Michelle S. Koo, Karen M.C. Rowe, Kevin C. Rowe, James L. Patton, John D. Perrine, Steven R. Beissinger, Craig Moritz

Biological Sciences

Species distribution models (SDMs) are commonly applied to predict species’ responses to anticipated global change, but lack of data from future time periods precludes assessment of their reliability. Instead, performance against test data in the same era is assumed to correlate with accuracy in the future. Moreover, high-confidence absence data is required for testing model accuracy but is often unavailable since a species may be present when undetected. Here we evaluate the performance of eight SDMs trained with historic (1900–1939) or modern (1970–2009) climate data and occurrence records for 18 mammalian species. Models were projected to the same or the …

Traversing Swanton Road, 13th Ed., James A. West

Academic, Government & Associated Organizational Research Resources

Situated at the northwest end of Santa Cruz County and occupying circa 30 square miles of sharply contrasted terrain, the Scott Creek Watershed concentrates within its geomorphological boundaries, at least 10-12% of California's flora, both native and introduced. Paradoxically, the use/abuse that the watershed has sustained over the past 140+ years, has not necessarily diminished the biodiversity and perhaps parallels the naturally disruptive but biologically energizing processes (fire, flooding, landslides and erosion), which have also been historically documented for the area for +60 years. This is an early edition of an extensive document by James A. West. Please visit the …

Synthesis And Evaluation Of Thermally-Responsive Coatings Based Upon Diels–Alder Chemistry And Renewable Materials, Dahlia N. Amato, Gregory A. Strange, John P. Swanson, Anton D. Chavez, Suzanne E. Roy, Kim L. Varney, Craig A. Machado, Douglas V. Amato, Philip Costanzo

Chemistry and Biochemistry

A soybean based coating with thermally responsive Diels–Alder linkages has been prepared following an automotive 2-component formulation. The resulting coatings displayed the capability to be healed following physical deformation by a thermal stimulus, and such a material has significant potential for end users. Various curing agents were employed, and resulted in variation of scratch resistance and re-healablity. Different thermally responsive soybean resins were synthesized to have varying amounts reversible and nonreversible linkages when incorporated into the coating. Additionally, different isocyanates were added at differing ratios of NCO:OH in search of the optimum coating. It was found through the analysis of …

Evaluation Of Fungicides For Control Of Dollar Spot Of Fairway Height Creeping Bentgrass In Kansas, 2012, C Thompson, Z Raundenbush, Megan Kennelly

Horticulture and Crop Science

No abstract provided.

Evaluation Of Granular Fungicides For Control Of Brown Patch Of Lawn Height Tall Fescue In Kansas, 2012, Cole Thompson, M. Kennelly

Horticulture and Crop Science

No abstract provided.

Zooplankton Trophic Ecology In The San Francisco Estuary During Summer As Determined By Stable Isotope Analysis, Kyla Bradylong, Steven Westbrook, Julien Moderan, Wim Kimmerer

STAR Program Research Presentations

Declines in the abundance of several pelagic fish species in the upper San Francisco Estuary have prompted investigation into food web interactions within the estuary and delta (the lower Sacramento and San Joaquin Rivers). This area is characterized by low primary production and pelagic food webs much longer and reticulated than previously thought, implying low efficiency in the energy transfers from primary producers to planktivorous fish. We determined the carbon and nitrogen stable isotope (SI) composition of zooplankton samples collected monthly between June 2012 and February 2013 at eight stations along the salinity gradient. As consumers SI composition reflects that …

Big Babies, Big Mammas?: Relationship Of Leatherback Hatchling Size And Mother Size, Shane Morales, Violet Campbell, Kelly Stewart

STAR Program Research Presentations

Individual leatherback hatchlings vary in size when compared to individuals from other nests, as well as individuals from the same nest. It is thought that many factors affect hatchling size but that one of the most influential factors is maternal size. Of all the aspects of a mother which could affect hatchling size, evidence for question concerning influence of mother size is determinable within the field using minimal tools and basic statistical analysis. If a direct correlation exists between mother size and hatchling size then the claim can be made that larger mothers produce larger offspring while smaller mothers produce …

Effects Of Composition And Separation Techniques On The Structure Of Aggregates In The Milk Fat Globular Membrane, Tristan Dilbeck, Jose Ibarra, Rafael Jimenez-Flores, Tracey Nguyen

STAR Program Research Presentations

The milk fat globular membrane (MFGM) is a complex structure composed mainly of phospholipids, sphingolipids, and membrane specific proteins. The composition and size of the MFGM differs based on the isolation, purification, and techniques used in analysis of the membrane as well as physiological, chemical, and mechanical factors present during its creation. The purpose of this experiment is to analyze the effects of separation techniques and composition of lipids and proteins on the structure of aggregates in the MFGM. The aggregates found in whey protein isolate (95% pure whey protein), whey buttermilk (whey protein and phospholipids), and sweet buttermilk (whey …

Abundance And Distribution Of Microplankton In The San Francisco Estuary, Carrie Ann Sharitt, Lindsay Sullivan, Wim Kimmerer

STAR Program Research Presentations

Microplankton are a diverse group of planktonic organisms ranging from 0.02 to 0.2 millimeters. Since the group is defined solely by size, it spans numerous taxonomic groups, including both heterotrophs and autotrophs. Microplankton are abundant in all aquatic ecosystems and are important prey for many organism, including bivalves, crustaceans, and fish. Little is known about the microplankton community in the San Francisco Estuary, and information on their abundance and distribution will help scientists better understand their role in local foodwebs. This research quantified the abundance and distribution of microplankton in the San Francisco Estuary from 2010 to 2012. Microplankton were …

Life Support: Long Term Storage Of Solid Waste In An Enclosed Membrane System, Emmy O. Trieu, Michael Flynn, Rocco Mancinelli

STAR Program Research Presentations

In deep space missions, maintaining life support is of the utmost priority. In such a closed system, human waste must be stored and treated. Simulated feces (ersatz) will be inoculated with microbes representing normal fecal flora, mixed with urine brine and shredded refuse of products typically used in space missions. Composting methods often use alternating layers of waste with scraps of carboniferous materials (finely shredded refuse). By preparing membrane bags with a homogenized ersatz and carboniferous refuse mixture and membrane bags with alternating layers of ersatz and carboniferous refuse, it may be possible to monitor anaerobic thermophillic digestion of the …

Identifying Molecular Markers Associated With Salt And Boron Tolerance In Poplar Trees, Darshanpreet Gill, Nathan Follen, Davis W. Cheng, Kyan Salehi, Gary Banuelos, James P. Prince

STAR Program Research Presentations

Standard irrigation practices promote salt and boron accumulation in soils on the Westside of Central California. The build-up of these inorganic salts leads to the degradation of arable land and reduction of crop production. Different clones of poplar trees have been shown to grow in salt- and boron-contaminated soils, and in doing so, gradually remove significant amounts of these inorganic salts from the soil. In an effort to identify molecular markers linked with salt and boron tolerance in poplars, 31 PCR primer pairs have been designed based on candidate genes identified from the literature, and an additional 31 PCR primer …

Testing Compounds As Sole Carbon Sources Of Strains Isolated From The Hot Lake Phototrophic Mat, Lyzbeth Becerra, Stephen Lindemann

STAR Program Research Presentations

Hot Lake is a meromictic, epsomitic lake. It has a phototrophic microbial mat that reassembles every year. The mat community is subjected to large fluctuations of salinity and solar irradiance. PNNL is interested in studying how these communities respond to the fluctuating environment conditions. To date, 70 isolates have been obtained from Hot Lake with the intention of characterizing their physiology to gain a better understanding of how they are naturally functioning in the Hot Lake mat community. I tested eight of these isolates in media designed to mimic the chemistry of Hot Lake with a selection of sugars, amino …

Creating A Package In R, Brit Schneiders, Eric Archer

STAR Program Research Presentations

In a time of increasingly efficient technology and data production, scientists are producing data faster than it can be analyzed. Therefore, user accessibility to data analysis is becoming more and more critical. In general, researchers have a set of raw data and want an efficient means to their final analysis. A package serves as that means by creating a set of functions and making them accessible to the user. Often, a user has a small piece of code to run (a single R script, for example), and that script requires the use of certain functions, which are contained in a …

Jellyfish Identification And Quantification In The San Francisco Estuary, Amalia Borson, Lindsay L. Sullivan, Wim Kimmerer

STAR Program Research Presentations

As potential predators and competitors of plankton-eating fish, jellyfish have the potential to negatively impact fish populations. Jellyfish were collected weekly with plankton tows from the RombergTiburonCenterpier in Tiburon, CA. Since some jellyfish were too small to identify, one tow was collected and preserved to record abundances, and a second tow was collected to rear jellyfish until distinguishing characteristics were visible enough for identification. Jellyfish in the preserved tows were then identified, measured, and counted, and their abundance (number m^-3) was calculated. Jellyfish from the second tows were reared in plastic buckets that were lightly bubbled using aquarium …

Preservation Of Biosignature Molecules In Potential Sample Return Container Of The Mars 2020 Mission, Kimberly E. Lykens, Fei Chen Ph.D

STAR Program Research Presentations

Preservation of Biosignature Molecules in Potential Sample Return Container of the Mars 2020 Mission

Kimberly Lykens¹ and Fei Chen²

¹Wittenberg University, Springfield, Ohio 45501 ²Jet Propulsion Laboratory, Pasadena, California, 91109

One requirement for sustainable life on terrestrial planets includes the presence of organic polymers, compounds that are essential for major biological functions such as replication and catalysis. An identified goal of the Mars mission in the year 2020 is to implement a sample-return to identify and validate signs of life on Mars through the discovery of biosignature molecules in Martian core samples. Martian core samples recovered …

Behavioral Variation Between Two Clades Of Leptasterias Spp., Ashley N. Contreras

STAR Program Research Presentations

Leptasterias spp. are six-rayed sea stars found along the rocky intertidal of the northeast Pacific Alaska to Santa Catalina Island, southern California. In central California, three clades of Leptasterias are found in separate or mixed populations, in diverse habitats that range from shallow pools of seagrass and algae to bare rock exposed to crashing waves. Initial field observations of two clades from different locations suggested that behavioral variation may relate to habitat differences among clades. To measure differences in activity, the righting response was timed at both field sites. As a result of behavioral variation observed in the field, more …

Temporal Variation In Larval Release In Botrylloides Violaceous, Damion J. Delton, Sarah Cohen

STAR Program Research Presentations

Despite Botrylloides violaceous being a globally invasive species in temperate marine habitats, little is known about it’s natural dispersal behavior. Previous work (Delton et al, 2011) suggests behavioral inferences based on a related and much more commonly studied species may not be appropriate, potentially due to a large size difference between the non-feeding larvae of these two species. Here, we consider factors that may affect dispersal potential. To study how light affects larval release, we measured timing of larval release in the field and compared larval size and time of release. Colonies were collected from floating docks in Richmond, CA …

Physiologically-Based Pharmacokinetic Modeling For Predicting Caffeine/Theophylline-Ciprofloxacin Interactions, David M. Ng, Ali Navid

STAR Program Research Presentations

Dynamics of interactions between the drugs caffeine, theophylline, and ciprofloxacin are predicted using physiologically-based pharmacokinetic (PBPK) modeling. Pharmacokinetic means the model determines where the drugs are distributed in the body over time. Physiologically-based means the anatomy and physiology of the human body are reflected in the structure and functioning of the model. Multiple drugs can interact to increase or decrease their beneficial and/or undesired effects. This is important because some common substances, such as caffeine in coffee, soft drinks, and energy drinks, are actually drugs that affect the body. Ciprofloxacin is an inhibitor of caffeine and theophylline metabolism; such inhibition …

Feeding Ecology Of Delta Smelt During A Seasonal Pulse Of Turbidity, William A. Hilton, Aaron Johnson, Wim Kimmerer

STAR Program Research Presentations

The delta smelt (Hypomesus transpacificus) is a small, pelagic fish endemic to the San Francisco Estuary (SFE) and protected under federal and state endangered species acts. This study examines the diet of adult delta smelt during their spawning migration in the winters of 2010 and 2012. Delta smelt and their zooplankton prey were sampled concurrently during a seasonal pulse of turbidity at sites along their migratory route from the low salinity zone in Suisun Bay to the fresher waters of the Sacramento/San Joaquin Delta. Gut contents were identified to the lowest possible taxon and counted, along with zooplankton …

Characterization Of Bacillus Bacterial Strains; Optimum Growth Temperature, Proteolytic And Lipolytic Activity, Jamie Anne Boring

STAR Program Research Presentations

Characterization of Bacillus bacterial strains is an important step in identifying and removing biofilms contaminating processing equipment. These biofilms lead to large amounts of spores in the final product. Strains that produce spoilage enzymes cause off-flavors, structural defects and reduced shelf life of milk products. Using four treatments, we determined the best growth protocol for each strain to use in future experiments. Treatment 1: 80 ^o C heat shock and 32 ^o C 24hr incubation, Treatment 2: 80^o C/ 55 ^o C, Treatment 3: 100 ^o C/ 32 ^o C, Treatment 4: 100 ^o C/ 55 ^o C. Our …

Leatherback Hatchling Fitness, Violet Campbell, Shane Morales, Kelly Stewart

STAR Program Research Presentations

Leatherback hatchling fitness on land is essential for their success in entering the ocean without being predated. Two ways fitness on land may be measured are by how fast hatchlings crawl as well as how fast their righting responses are when they are flipped onto their carapaces. Hatchlings from certain nests have slower righting response times along with slower crawl times, both of which would increase the odds of predation and hinder the success of hatchlings reaching the ocean.

Hatchling crawling speed was determined by timing 12 randomly selected hatchlings from each nest to crawl a distance of two meters. …

Juxtaposing Nasa’S Aeronet Aod With Carb Pm Data Over The San Joaquin Valley To Facilitate Multi-Angle Imaging Spectroradiometer (Misr) Pm Pollution Research, John Kanemoto

STAR Program Research Presentations

Airborne particulate matter (PM) has been shown to increase the risk for asthma, chronic bronchitis, cardiopulmonary complications, and respiratory cell membrane damage/infection/leakage. PM levels are currently analyzed from two perspectives: stationary land-based monitoring (LBM) sites and total Aerosol Optical Depth (AOD) atmospheric column measurements. Both perspectives often leave miles of space between measuring locations and will have a continually increasing cost from introducing/maintaining sites. The Multi-angle Imaging SpectroRadiometer (MISR) satellite team hopes to begin investigating/archiving PM levels comprehensively via inputting MISR AOD measurements into a function/model which predicts the amount of ground level PM.

In the future, multivariable spatial correlations …

Effect Of Adding Salt During The Diafiltration Step Of Milk Protein Concentrate Powder Manufacture On Mineral And Soluble Protein Composition, Vandna Sikand, Phillip S. Tong, John Walker

Dairy Science

Milk protein concentrate (MPC) powders offer a great potential for use in an array of food applications because of their nutritional and functional values. However, MPC powders with protein content ≥80% (MPC80) exhibit poor solubility and hence restrict their potential use. The objective of this study was to determine the impact of adding salt during the diafiltration stage of MPC80 manufacture on solubility, turbidity, and to compare minerals and protein content of supernatants of ultracentrifuged samples with control sample. Three types of samples were produced: MPC80-C (control) with or without salt treatment, MPC80-Na (150 mM NaCl), and MPC80-K (150 mM …