Life Sciences Commons^™

Expression And Characterization Of The Flocculin Flo11/Muc1, A Yeast Mannoprotein With Homotypic Properties Of Adhesion, Li Li Ph.D., Lois M. Douglas, Yang Yang, A M. Dranginis

Faculty Works: Biology, Chemistry, and Environmental Studies

The Flo11/Muc1 flocculin has diverse phenotypic effects. Saccharomyces cerevisiae cells of strain background Σ1278b require Flo11p to form pseudohyphae, invade agar, adhere to plastic, and develop biofilms, but they do not flocculate. We show that S. cerevisiae var. diastaticusstrains, on the other hand, exhibit Flo11-dependent flocculation and biofilm formation but do not invade agar or form pseudohyphae. In order to study the nature of the Flo11p proteins produced by these two types of strains, we examined secreted Flo11p, encoded by a plasmid-borne gene, in which the glycosylphosphatidylinositol anchor sequences had been replaced by a histidine tag. A protein of …

Determining Domain Similarity And Domain-Protein Similarity Using Functional Similarity Measurements Of Gene Ontology Terms, Lisa Michelle Guntly, Jennifer Leopold, Anne M. Maglia

Computer Science Faculty Research & Creative Works

Protein domains typically correspond to major functional sites of a protein. Therefore, determining similarity between domains can aid in the comparison of protein functions, and can provide a basis for grouping domains based on function. One strategy for comparing domain similarity and domain-protein similarity is to use similarity measurements of annotation terms from the Gene Ontology (GO). In this paper five methods are analyzed in terms of their usefulness for comparing domains, and comparing domains to proteins based on GO terms.

Crop Updates 2007 - Cereals, R. Loughman, R. Lance, I. Barclay, G. Crosbie, S. Harasymow, W. Lambe, C. Li, R. Mclean, C. Moore, K. Stefanova, A. Tarr, R. Wilson, Matu Peipi, Matt Whiting, Christine Zaicou, Shahajahan Miyan, Brenda Shackley, Len J. Wade, Lindsay W. Bell, Felicity Byrne (Nee Flugge), Mike A. Ewing, Blakely Paynter, Andrea Hills, Raj Malik, Kelly Winfield, Harmohinder Dhammu, Vince Lambert, Chris Roberts, Jeromy Lemon, Geoff Thomas, Ian Hartley, Andrew Taylor, Manisha Shankar, John Majewski, Vivien Vanstone, Brenda Coutts, Monica Kehoe, Roger Jones, Geoffrey Dwyer, Belinda Welsh, Cuiping Wang, Linda Price

Crop Updates

This session covers twenty six papers from different authors:

CEREAL BREEDING

1. Strategies for aligning producer and market imperatives in cereal breeding in Western Australia, R. Loughman, R. Lance, I. Barclay, G. Crosbie, S. Harasymow, W. Lambe, C. Li, R. McLean, C. Moore, K. Stefanova, A. Tarr and R. Wilson, Department of Agriculture and Food

2. LongReach plant breeders wheat variety trials – 2006, Matu Peipi and Matt Whiting, LongReach Plant Breeders

WHEAT AGRONOMY

3. Response of wheat varieties to sowing time in the northern agricultural region in 2006, Christine Zaicou, Department of Agriculture and Food

4. Response …

Coupling Coherence Distinguishes Structure Sensitivity In Protein Electron Transfer, Tatiana Prytkova, Igor V. Kurnikov, David Beratan

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Quantum mechanical analysis of electron tunneling in nine thermally fluctuating cytochrome b562 derivatives reveals two distinct protein-mediated coupling limits. A structure-insensitive regime arises for redox partners coupled through dynamically averaged multiple-coupling pathways (in seven of the nine derivatives) where heme-edge coupling leads to the multiple-pathway regime. A structure-dependent limit governs redox partners coupled through a dominant pathway (in two of the nine derivatives) where axial-ligand coupling generates the single-pathway limit and slower rates. This two-regime paradigm provides a unified description of electron transfer rates in 26 ruthenium-modified heme and blue-copper proteins, as well as in numerous photosynthetic proteins.

Anisotropic Atomic Motions In High-Resolution Protein Crystallography Molecular Dynamics Simulations, Conrad J. Burden, Aaron J. Oakley

Faculty of Science - Papers (Archive)

Molecular dynamics (MD) simulations using empirical force fields are popular for the study of proteins. In this work, we compare anisotropic atomic fluctuations in nanosecond-timescale MD simulations with those observed in an ultra-high-resolution crystal structure of crambin. In order to make our comparisons, we have developed a compact graphical technique for assessing agreement between spatial atomic distributions determined by MD simulations and observed anisotropic temperature factors.

Effect Of Protein Stabilization On Charge State Distribution In Positive- And Negative Ion Electrospray Ionization Mass Spectra, Stephen J. Watt, Margaret Sheil, Jennifer L. Beck, Pavel Prosselkov, Gottfried Otting, Nicholas E. Dixon

Faculty of Science - Papers (Archive)

Changes in protein conformation are thought to alter charge state distributions observed in electrospray ionization mass spectra (ESI-MS) of proteins. In most cases, this has been demonstrated by unfolding proteins through acidification of the solution. This methodology changes the properties of the solvent so that changes in the ESI-MS charge envelopes from conformational changes are difficult to separate from the effects of changing solvent on the ionization process. A novel strategy is presented enabling comparison of ESI mass spectra of a folded and partially unfolded protein of the same amino acid sequence subjected to the same experimental protocols and conditions. …

Changes In Energy Expenditure With Ingestion Of High Protein, High Fat Versus High Protein, Low Fat Meals Among Underweight, Normal Weight, And Overweight Females., Amy Jo Riggs, Sareen S. Gropper, Barry D. White

Department of Health Sciences and Kinesiology Faculty Publications

Background: Metabolic rate is known to rise above basal levels after eating, especially following protein consumption. Yet, this postprandial rise in metabolism appears to vary among individuals. This study examined changes in energy expenditure in response to ingestion of a high protein, high fat (HPHF) meal versus an isocaloric high protein, low fat (HPLF) meal in underweight, normal weight, or overweight females (n = 21) aged 19–28 years.

Methods: Energy expenditure, measured using indirect calorimetry, was assessed before and every 30 minutes for 3.5 hours following consumption of the meals on two separate occasions. Height and weight were …