Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Identification Of Three Randomly Amplified Polymorphic Dna-Polymerase Chain Reaction Markers For Distinguishing Asian And North American Gypsy Moths (Lepidoptera: Lymantriidae), David E. Schreiber, Karen J. Garner, James M. Slavicek
Identification Of Three Randomly Amplified Polymorphic Dna-Polymerase Chain Reaction Markers For Distinguishing Asian And North American Gypsy Moths (Lepidoptera: Lymantriidae), David E. Schreiber, Karen J. Garner, James M. Slavicek
USDA Forest Service / UNL Faculty Publications
Gypsy moths originating in Asia have recently been introduced into North America, making it necessary to develop markers for distinguishing the Asian strain from the established North American population. We have identified 3 randomly amplified polymorphic DNA-polymerase chain reaction generated (RAPD-PCR) markers which are specific for either Asian or North American moths. The FS-2 and FS-3 markers are specific for the North American strain, and the FS-4 marker is specific for moths from far east Asia. The 3 RAPD-amplified fragments have been cloned and sequenced, and locus-specific primers have been developed for the FS-3 marker. These markers, in combination with …
Molecular Analysis Of An Enhancin Gene In The Lymantria Dispar Nuclear Polyhedrosis Virus, David S. Bischoff, James M. Slavicek
Molecular Analysis Of An Enhancin Gene In The Lymantria Dispar Nuclear Polyhedrosis Virus, David S. Bischoff, James M. Slavicek
USDA Forest Service / UNL Faculty Publications
A Lymantria dispar nuclear polyhedrosis virus (LdMNPV) gene has been identified that encodes a homolog to the granulovirus (GV) enhancin proteins that are capable of enhancing the infection of other baculoviruses. Enhancin genes have been identified and sequenced for three species of GVs but have not been found in any other nuclear polyhedrosis virus to date. The LdMNPV enhancin gene is located between 67.6 and 70.1 kbp on the viral genome. Northern and primer extension analyses of viral RNAs indicate that the enhancin gene transcripts are expressed at late times postinfection from a consensus baculovirus late promoter. The LdMNPV enhancin …
Phenotypic And Genetic Analysis Of Lymantria Dispar Nucleopolyhedrovirus Few Polyhedra Mutants: Mutations In The 25k Fp Gene May Be Caused By Dna Replication Errors, David S. Bischoff, James M. Slavicek
Phenotypic And Genetic Analysis Of Lymantria Dispar Nucleopolyhedrovirus Few Polyhedra Mutants: Mutations In The 25k Fp Gene May Be Caused By Dna Replication Errors, David S. Bischoff, James M. Slavicek
USDA Forest Service / UNL Faculty Publications
We previously demonstrated that polyhedron formation (PF) mutants arise at a high frequency during serial passage of the Lymantria dispar nucleopolyhedrovirus (LdMNPV) in the L. dispar 652Y cell line (J. M. Slavicek, N. Hayes-Plazolles, and M. E. Kelly, Biol. Control 5:251–261, 1995). Most of these PF mutants exhibited the traits of few polyhedra (FP) mutants; however, no large DNA insertions or deletions that correlated with the appearance of the FP phenotype were found. In this study, we have characterized several of the PF mutants at the phenotypic and genetic levels. Genetic techniques were used to group the mutations in the …
Characterization Of The Replication Cycle Of The Lymantria Dispar Nuclear Polyhedrosis Virus, Christopher I. Riegel, James M. Slavicek
Characterization Of The Replication Cycle Of The Lymantria Dispar Nuclear Polyhedrosis Virus, Christopher I. Riegel, James M. Slavicek
USDA Forest Service / UNL Faculty Publications
The life cycle of the Lymantria dispar nuclear polyhedrosis virus (LdMNPV) was characterized through analysis of budded virus (BV) release, the temporal formation of polyhedra, the temporal transcription pattern of representative early, late, and hyper-expressed late genes, and the onset of DNA replication in the Ld652Y cell line. Transcripts from the LdMNPV immediate early gene G22 were detected 4 h post infection (h p.i.). The late and hyper-expressed late p39 capsid and polyhedrin genes were initially transcribed at approximately 20 and 24 h p.i., respectively. Viral DNA replication initiated at approximately 18-20 h p.i. Budded virus …