Life Sciences Commons^™

Cellular Iron Storage And Trafficking Are Affected By Gtn Stimulation In Primary Glial And Meningeal Cell Culture, Lati̇fe Arzu Aral, Mehmet Ali̇ Ergün, Hayrunni̇sa Bolay Belen

Turkish Journal of Biology

A well-balanced intracellular iron trafficking in glial cells plays a role in homeostatic processes. Elevated intracellular iron triggers oxidative stress and cell damage in many neurological disorders, including migraine. This study aimed to investigate the effects of glyceryl trinitrate (GTN), on cellular iron homeostasis, matrixmetalloproteinase (MMP)-9, and calcitonin gene related peptide (CGRP) receptor (CRLR/CGRPR1) production in microglia, astrocyte, and meningeal cell cultures. Primary glial and meningeal cells in culture were exposed to GTN for 24 h. Messenger RNA expression was assessed using qPCR. Iron accumulation was visualized via modified Perl?s histochemistry. MMP-9 levels in cell culture supernatants were measured using …

Effects Of Iron Injection Timing On Suckling And Subsequent Nursery And Growing-Finishing Performance And Hematological Criteria Under Commercial Conditions, H. E. Williams, B. Carrender, R. Maurer, J. M. Derouchey, J. C. Woodworth, S. S. Dritz, M. D. Tokach, R. D. Goodband, A. J. Holtcamp, K. F. Coble

Kansas Agricultural Experiment Station Research Reports

A total of 1,892 newborn pigs (PIC 359 × C40; initially 3.3 lb body weight [BW]) within 172 litters were used in a 168-d study to evaluate the effects of Fe injection timing after birth on suckling and subsequent nursery and growing-finishing growth performance and hematological criteria. One day after birth, piglets were weighed, and 11 pigs within each litter were allotted to 1 of 6 treatments consisting of no Fe injection or 200 mg of injectable Fe provided on d 1, 3, 5, or 7 of age, or 200-mg on d 1 plus 200-mg on d 12 of age. …

Expression Of Soluble, Active, Fluorescently Tagged Hephaestin In Cos And Cho Cell Lines, Eli̇f Si̇bel Aslan, Kenneth N. White, Basharut A. Syed, Kaila S. Srai, Robert W. Evans

Turkish Journal of Biology

Hephaestin (Hp) is a trans-membrane protein, which plays a critical role in intestinal iron absorption. Hp was originally identified as the gene responsible for the phenotype of sex-linked anaemia in the sla mouse. The mutation in the sla protein causes accumulation of dietary iron in duodenal cells, causing severe microcytic hypochromic anaemia. Although mucosal uptake of dietary iron is normal, export from the duodenum is inhibited. Hp is homologous to ceruloplasmin (Cp), a member of the family of multi copper ferroxidases (MCFs) and possesses ferroxidase activity that facilitates iron release from the duodenum and load onto the serum iron transport …

Treating Anemia Of Chronic Disease, Sarit Fischer

The Science Journal of the Lander College of Arts and Sciences

Anemia of chronic disease (ACD) is the result of altered iron metabolism, blunted erythropoiesis, and shortened red blood cell survival caused by inflammatory cytokines. Hepcidin plays a key role in anemia of chronic disease by inducing endocytosis of the iron exporter ferroportin. Iron is trapped in enterocytes, hepatocytes, and macrophages, and is unavailable for hemoglobin synthesis. When treating the underlying disease is not an option, or anemia is severe, ACD should be treated. While oral iron supplementation is not suitable for ACD, intravenous iron may be effective. Erythropoiesis stimulating agents are a common approach to treating ACD, but a large …

Effects Of Feeding Increasing Levels Of Iron From Iron Sulfate Or Iron Carbonate On Nursery Pig Growth Performance And Blood Criteria, H. E. Williams, J. C. Woodworth, J. M. Derouchey, S. S. Dritz, M. D. Tokach, R. D. Goodband, J. Usry

Kansas Agricultural Experiment Station Research Reports

A total of 140 weanling pigs (DNA 241 × 600, initially 12.2 ± 0.02 lb) were used in a 32-d study evaluating the effects of increasing dietary iron from either iron sulfate (FeSO₄) or a micronized, agglomerated ferrous carbonate (FeCO₃) on nursery pig growth performance and blood criteria. The micronized form of FeCO₃is designed to improve nursery pig growth performance and blood iron status. Pigs used for this trial did not receive an iron injection after birth in order to increase sensitivity to added dietary iron. Pigs were weaned at approximately 21 d and …

Exploring The Value Of Plant Analysis To Enhance Water Use Efficiency In Southwest Kansas, A. J. Foster, I. Kisekka, B. Golden

Kansas Agricultural Experiment Station Research Reports

Nutrient deficiency is identified by use of visual symptoms. However, the application of the proposed deficient nutrient often does not result in the correction of the observed visual symptoms. This is because essential nutrients do not operate independently of each other or independently of the overall plant health and growing conditions. A study was initiated in 2016 at the Kansas State University Southwest Research-Extension Center Finnup Farm near Garden City, KS, to use both soil and plant analyses to identify toxicities or hidden deficiencies that could be limiting corn yield at various irrigation capacities. Soil samples prior to planting and …

Qtls For Iron Concentration In Seeds Of The Cultivated Lentil (Lens Culinaris Medic.) Via Genotyping By Sequencing, Seçi̇l Aldemi̇r, Duygu Ateş, Hülya Yilmaz Temel, Bülent Yağmur, Ahmad Alsaleh, Abdullah Kahri̇man, Hakan Özkan, Albert Vandenberg, Muhammed Bahatti̇n Tanyolaç

Turkish Journal of Agriculture and Forestry

Lentil, Lens culinaris Medic., is an important cool season grain legume because of the high level of iron (Fe) in its seeds since Fe deficiency is widespread and causes anemia. Thus, identifying genes controlling Fe concentration in the seed was needed for mapping in the lentil genome. The objectives of this study were to (i) detect phenotypic variation in Fe concentration in the seeds of a recombinant inbred line (RIL) population, (ii) construct a high-density linkage map using genotyping by sequencing (GBS), and (iii) identify localization of the quantitative trait loci (QTLs) controlling genes for Fe concentration in lentil seeds. …

Confirming The Spatial Relationship Between Iron, Tau Protein, And Amyloid Beta Within The Subiculum As A Means To Better Detect Alzheimer’S Disease With Magnetic Resonance Imaging, Brandon Herrington, Jonathan Wisco

Journal of Undergraduate Research

As the 6^th leading cause of death in the United States¹, and because of the significant deterioration it causes in the quality of life of its victims, Alzheimer’s disease is an area where research to improve its early diagnosis is paramount to establishing new treatments. Alzheimer’s disease appears to show its effects at a particularly early stage within the hippocampus². More specifically, the subicular area of the hippocampus is noted as a region that is subjected to more severe pathological changes due to the disease³. Braak staging is a method used to classify, …

Copper Enriches Efficacy Of Dp44mt In Breast Cancer Cells, Ufuk Özer

Turkish Journal of Biology

Neoplastic cells need essential metals, such as iron and copper, for cellular functions and rapid growth. Metal chelation and changes in their redox cycle in favor of oxidative stress may be critical for making these cells vulnerable to cell death. The HER2-overexpressing breast cancer cell line, MCF7-HER2, and its vehicle control, MCF7-vec cells, were treated with CuCl2, FeSO4, and Dp44mT. Reactive oxygen species (ROS) production in response to these chemicals was detected with flow cytometry, and cell viability was measured by MTT assay in the cells. ROS levels were relatively increased by Dp44mT in the cells, and this was reversed …

Iron As A Biomarker For Alzheimer’S Disease, Samual Barlow, Dr. Jonathan Wisco

Journal of Undergraduate Research

Alzheimer’s Disease (AD) is one of the highest causes of death in the United States. After the age of 65, the chance of getting Alzheimer’s doubles every five years. As the average lifespan of Americans increases, the importance of understanding AD and finding more efficient ways to treat it increases as well. The earlier AD is treated, the more effectively we are able to treat it. Non-heme iron (Fe) has been shown to spatially correlate with Abeta. Since Fe causes a signal dropout in susceptibility-weighted Magnetic Resonance Imaging (MRI), this imaging modality could possibly be used as a way to …

Concentrations Of Manganese, Iron, And Strontium In Bones Of Thedomestic Dog (Canis Lupus Familiaris), Halina Budis, Elzbieta Kalisinska, Natalia Lanocha, Danuta Kosik_Bogacka

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine concentrations of manganese (Mn), iron (Fe), and strontium (Sr) in the cartilage, compact bone, spongy bone, and cartilage with adjacent compact bone of the domestic dog (Canis lupus familiaris). The experiment was carried out on bones from the hip joints of 24 dogs (dogs of 8 years) from northwestern Poland. The concentrations of Mn, Fe, and Sr were assessed by atomic absorption spectrophotometry. Fe concentration was the highest in the cartilage and compact bone. Mn concentration was the lowest in all three types of bone material and had a significantly lower concentration …

Characterizing The Expression Of Genes Involved In Iron Transport Inpakistani Peanut Varieties Under Iron Deficiency Stress, Shamim Akhtar, Yusuke Kakei, Khurrum Bashir, Armghan Shahzad, Takashi Yamakawa, Muhammad Arshad, Fayyaz- Ul-Hassan, Hiromi Nakanishi, Naoko K. Nishizawa

Turkish Journal of Botany

Iron (Fe) deficiency is one of the major yield-limiting factors in peanut (Arachis hypogaea L.), especially when grown in calcareous soils. The soils of the Pothwar region in Pakistan are calcareous in nature, exposing peanut to Fe deficiency. The molecular mechanisms governing Fe deficiency response in peanut have not been fully revealed. We compared 4 locally important varieties of peanut to evaluate Fe deficiency responses at the molecular level. The expression profiles of 7 important genes including AhIRT1, AhFRO1, AhNRAMP1, AhYSL1, AhYSL3.1, AhYSL4, and AhYSL6 in roots and young and old leaves of local peanut varieties were investigated 19 days …

In Vitro Screening Of Octoploid Fragaria Chiloensis And Fragaria Virginiana Genotypes Against Iron Deficiency, Ayfer Alkan Torun, Yildiz Kaçar, Belgi̇n Bi̇çen, Nazi̇fe Erdem, Sedat Serçe

Turkish Journal of Agriculture and Forestry

Iron (Fe) deficiency is one of the common problems in strawberry (Fragaria × ananassa Duch.) growing regions. In vitro screening enables researchers to evaluate genotype performance under several concentrations of Fe in a relatively fast and economical way. Therefore, we evaluated the responses of several genotypes belonging to octoploid Fragaria chiloensis (L.) Mill. and Fragaria virginiana Mill. (the progenitors of cultivated strawberry) against Fe treatments under in vitro conditions. In the first experiment, HM 1, Pigeon Point, CFRA1267 (F. chiloensis subsp. pacifica), and CA1541 (F. chiloensis subsp. chiloensis) were tested with various Fe concentrations (0%, 10%, 15%, 25%, 50%, 65%, …

Nitrate And Iron Nutrition Effects On Some Nitrate Assimilation Enzymes And Metabolites In Spirulina Platensis, Merve Esen, Razi̇ye Öztürk Ürek

Turkish Journal of Biology

Effects of different sodium nitrate and iron concentrations on the production of some metabolites and pigments and the activities of some nitrate assimilation enzymes [nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), and glutamate synthase (GOGAT)] were investigated in the growth medium of Spirulina platensis (Gamont) Geitler. For this purpose, S. platensis was cultivated on Zarrouk's medium containing different sodium nitrate concentrations (10-180 mM). A 100 mM sodium nitrate concentration stimulated growth of this organism as well as the production of pigments and other metabolites and enzyme activities. In the presence of 100 mM sodium nitrate, different concentrations of …

Perbandingan Zat Besi Dengan Dan Tanpa Vitamin C Terhadap Kadar Hemoglobin Wanita Usia Subur, Tuti Anggriani Utama, Nora Listiana, Desi Susanti

Kesmas

Anemia pada wanita pekerja masih merupakan masalah kesehatan yang dapat menurunkan produktivitas kerja. Penelitian ini bertujuan untuk membandingkan zat besi dengan dan tanpa vitamin C terhadap kadar hemoglobin. Jenis penelitian yang digunakan yaitu Quasy Experimental dengan Pre Test and Post Test Control Group Design. Populasi penelitian berjumlah 600 orang dan sampel berjumlah 60 orang. Teknik pengambilan sampel random sampling. Pengumpulan data dilakukan dengan cara pengamatan, wawancara, pemeriksaan hemoglobin, dan data sekunder. Pada kelompok perlakuan diberi tablet zat besi dan Vitamin C, pada kelompok kontrol hanya diberi tablet zat besi. Intervensi yang dilakukan adalah pemberian tablet zat besi dengan dan tanpa …

Changes In Transferrin Gene Expression In Sea Bass (Dicentrarchus Labrax) Challenged With Vibrio Anguillarum, Menekşe Di̇dem Ercan, Süheyla Karataş, Emre Turgay, Mustafa Kolukirik, Orhan İnce, Bahar İnce

Turkish Journal of Veterinary & Animal Sciences

Vibrio anguillarum expresses several virulence factors and causes hemorrhagic septicemia accompanied by serious losses in marine fish. Transferrin is a glycoprotein, also known as a multitasking protein, which is mainly synthesized by the liver. It has a fundamental role in the immune system. In the present study, the transferrin gene expression of sea bass (Dicentrarchus labrax) was investigated during an experimental infection with V. anguillarum. Fish samples were examined by hematological and serological methods as well as real-time polymerase chain reaction. The infection was performed via water. The sampled fish displayed vibriosis infection symptoms, both internally and externally. The transferrin …

Serum Iron, Total Iron-Binding Capacity, Unbound Iron-Binding Capacity, Transferrin Saturation, Serum Copper, And Hematological Parameters In Pregnant Akkaraman Ewes Infected With Gastro-Intestinal Parasites, Süleyman Kozat, Nazmi̇ Yüksek, Yaşar Göz, İhsan Keleş

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate serum iron parameters, and copper and iron deficiency anemia in ewes infected with gastro-intestinal parasites. This study was conducted with 60 pregnant Akkaraman ewes. In the statistical analyses, values obtained from both parasite infected and uninfected ewes were compared. Significant decreases were observed in PCV (P < 0.05), Hb (P < 0.05), RBC (P < 0.05), total protein (P < 0.05), albumin (P < 0.05), globulin (P < 0.05), and MCHC (P < 0.01), and a significant increase was seen in the WBC value (P < 0.05) in the parasite infected group. Furthermore, the parasite infected group had lower serum Fe (P < 0.001), TS % (P < 0.001), and serum Cu (P < 0.01) levels, and had higher UIBC values (P < 0.05) compared to the values obtain from the uninfected group. Therefore, in addition to antiparasite drug treatment in parasite infected pregnant ewes, iron preparations should also be administered in order to treat iron deficiency.

Investigation Of Serum Cu, Zn, Fe And Se Concentrations During Pregnancy (60, 100 And 150 Days) And After Parturition (45 Days) In Single And Twin Pregnant Sheep, Fuat Gürdoğan, Ati̇lla Yildiz, Engi̇n Balikçi

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the serum Cu, Zn, Se and Fe concentrations at 60, 100, and 150 days of pregnancy and at 45 days of parturition in twin and single pregnant sheep. A total of 30 pregnant Akkaraman sheep in 2 groups (15 single pregnancies, 15 twin pregnancies), aged 3-5 years and weighing 40-50 kg were used. The blood samples were analyzed for serum Cu, Zn, Se and Fe concentrations. No statistically significant difference was found for Se, Cu, Fe and Zn concentrations between the twin and single pregnancy groups. Insignificant decreases (P > 0.05) in serum Zn and …

Effects Of Deboning Methods On Chemical Composition And Some Properties Of Beef And Turkey Meat, Meltem Serdaroğlu, Gülen Turp Yildiz, Neri̇man Bağdatlioğlu

Turkish Journal of Veterinary & Animal Sciences

The objective of this research was to compare some chemical and physical characteristics of mechanically deboned and hand deboned turkey meat and beef. Samples were analyzed for proximate composition, cholesterol, TBA, calcium and iron contents, hunter colour parameters and fatty acid composition. Deboning method affected chemical composition of beef and turkey meat. Mechanical deboning resulted higher cholesterol values and calcium and iron content. In hand deboned turkey meat the most abundant fatty acids were C16:0, C18:1 and C18:2. Mechanical deboning process was increased the percent of C18:1, C18:2 and C18:3 in turkey meat. C16:0, C18:0 and C18:1 were the major …

Morphology Studies Of Iron-Manganese Thin Films, G. Mathew, K. -W. Ng, A. R. Sethuraman, J. M. Stencel

Scanning Microscopy

Fe-based catalysts are known to be effective for Fischer-Tropsch synthesis from coal but are sensitive to sulfur poisoning. Addition of manganese to these catalysts has been proposed in an effort to combat this catalyst deactivation. To investigate the fundamental physical aspects of Mn incorporation into Fe, different compositions of model thin films of Fe-Mn, ranging from 100% Fe to 100% Mn were studied for the very first time, using scanning tunneling microscopy (STM), atomic force microscopy (AFM) and scanning electron microscopy. Our preliminary results indicate that the grain size of iron varied from 50 nm to 150 nm using the …

Proton-Induced X-Ray Emission Spectrometry In Archaeology, C. P. Swann, S. J. Fleming

Scanning Microscopy

Proton-induced x-ray emission (PIXE) spectrometry is fast developing a reputation as a powerful analytical tool in the study of a range of ancient materials, including bronze, iron, gold, glass, faience, and smelting slag. PIXE data allows determination of the primary constituents which would indicate their recipe of production and determine their bulk physical properties (e.g., color of a glass, brittleness in a metal), and of a wide range of trace elements which may indicate the source of raw material s from which an artifact was constituted. Over the past seven years, PIXE spectrometry's primary advantage over other recognized methods now …

Iron Deficiency Of Oats In The Lower South West, Edgar Noel Fitzpatrick, R. Sprivulis

Journal of the Department of Agriculture, Western Australia, Series 4

Oats grown on the acid peaty sands of the West and South Coastal areas of Western Australia develop iron deficiency.

This deficiency can be cured by spraying with iron sulphate. A 2 to 4 per cent, solution can be applied through a low volume spray without damage to the plants. The spray should be repeated when deficiency symptoms reappear.

Growing oats on these soils is not recommended.