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Reply By A. Carretero Et Al., Ana Carretero, H. Ditrich, M. Navarro, H. Splechtna, J. Ruberte

Scanning Microscopy

Dear Editor,

The study of vascular casts in the embryo by means of the scanning electron microscope represents the main subject of our research. We fully agree with DeRuiter and Gittenberger-de Groot in that corrosion casting is a valuable tool to study the early angiogenesis in embryos.

Letter To The Editor By M.C. Deruiter And A.C. Gittenberger-De Groot Relating To: "Technical Improvements In Corrosion Casting Of Small Specimens: A Study On Mesonephric Tubules And Vessels Of Chicken Embryos." [By A. Carretero, H. Ditrich, M. Navarro, H. Splechtna, J. Ruberte, Scanning Microscopy Vol. 7(4), P. 1333-1338 (1993)]., M. C. Deruiter, A. C. Gittenberger-De Groot

Scanning Microscopy

Dear Editor,

The topic of scanning electron microscopy (SEM) investigation of casting of vessels in the embryo still holds our interest. We are particularly focused on the possibilities of early injection as a method to support angiogenesis and vasculogenesis research in the embryo. It is questionable whether our technique leads to unreliable results because of severe trauma to the embryo as suggested by the above mentioned authors. The lower success yield might very well be the result of the higher friability of the embryo in general in this young stage.

Diluted And Undiluted Mercox Severely Destroy Unfixed Endothelial Cells. A Light And Electron Microscopic Study Using Cultured Endothelial Cells And Tadpole Tail Fin Vessels, J. Gassner, A. Lametschwandtner, T. Weiger, H. C. Bauer

Scanning Microscopy

Mercox is a methylmethacrylate-based resin which is widely used for vascular corrosion casting with subsequent scanning electron microscopic analysis. In the present study the effect of undiluted and diluted Mercox (4+1; volume + volume; Mercox: monomeric methylmethacrylate (MMA); 0.02 g catalyst MA/ml Mercox) and methylmethacrylate with and without catalyst MA (0.625 g/10 ml MMA) on fixed and unfixed endothelial cells was studied. Light microscopy (LM) of cultured capillary endothelial cells (ECs), which were replicated with diluted or undiluted Mercox shows degranulation and membrane perturbation of ECs, while no morphological changes occur in glutaraldehyde-prefixed ECs. Scanning electron microscopy (SEM) of replicas …

Microvascular Corrosion Casting Of The Rat Mandibular Joint: A Technical Approach, Etienne Piette, Alois Lametschwandtner

Scanning Microscopy

The rat mandibular joint is a ginglymoarthrodial joint deeply hidden under the zygomatic process of the squamosal bone. This joint was studied by scanning electron microscopy (SEM) of microvascular corrosion casts in 47 adult rats. Six additional rats were injected with India ink and thick sections of the joint area were examined by light microscopy. The small size of the joint (2.5 mm x 1.5 mm in occlusion), the existence of two slit-like joint compartments, the close proximity of bones and soft tissues in a mobile structure, and the intimate relationships of the joint with adjacent skeletal muscles made the …

Different Forms Of Corrosion Casts, Seyedhossein Aharinejad, Peter Böck

Scanning Microscopy

We have previously described artifacts in corrosion casts prepared with Mercox®, diluted with methylmethacrylic acid (MMA). Using scanning electron microscopy (SEM) of casts and light microscopy (LM) of tissue sections, we found new forms of artifacts in casts prepared with both undiluted and MMA-diluted Mercox®. When undiluted Mercox® was used, most of the casts did not show artifacts. Artifacts were rarely seen and comprised empty casts consisting of a rim, while endothelial cell nuclear imprints and other vascular wall structures were replicated. Other casts sometimes showed vessels with a fine granular surface or some with conglomerates in their internal structure, …

The Microvasculature Of The Larynx: A Scanning Electron Microscopic Study, Peter Franz, Seyedhossein Aharinejad

Scanning Microscopy

In ten adult guinea pigs, the microvascular architecture of the larynx was evaluated using microvascular corrosion casts and scanning electron microscopy. The vocal cords were provided with a subepithelial capillary network. The capillaries, freely anastomosing with each other, were supplied and drained via strongly undulating arteries and veins. The undulation of the latter vessels may explain their adaptability to volume changes of the larynx during phonation. The vasculature of the internal perichondrium of the thyroid cartilage was interrupted at the anterior commissure where an avascular zone was present at the origin of the vocal cords. This avascular area is common …

The Microvasculature Of Human Infant Oral Mucosa Using Vascular Corrosion Casts And India Ink Injection Ii. Palate And Lip, Q. X. Yu, K. M. Pang, W. Ran, H. P. Philipsen, X. H. Chen

Scanning Microscopy

The microvasculature of human hard and soft palate and lip originating from four infant males and six females, aged 6 months to 2 years was studied by scanning electron microscopy of vascular corrosion casts and light microscopy of India ink injected specimens. The capillary loops of the hard palate mucosa and vermilion border of the lips were found to be tall, numerous and consisted of primary, secondary and tertiary loops. Those of the soft palatal and labial mucosa were short, few in number and demonstrated a simple hair-pin shape originating directly from the subpapillary vascular network. It was concluded that …

Microvascular Pattern Of The Retina In The Japanese Monkey (Macaca Fuscata Fuscata), Shigenori Okada, Yoshikuni Ohta

Scanning Microscopy

The microvascular architecture of the retina in the Japanese monkey was revealed using scanning electron microscopy of microvascular corrosion casts.

At the optic nerve disc, the central retinal artery radiated to four primary retinal arterioles: the superior and inferior temporal arterioles and superior and inferior nasal arterioles. A dense retinal capillary network showed regional differences in density. The capillary networks observed were divided in four parts: the optic nerve disc, macula, equator, and ora serrata. At the optic nerve disc, the network was a multi-laminar structure containing the radial peripapillary capillaries in parallel with the nerve fibers at the innermost …