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Journal

1976

Biochemistry

Articles 1 - 4 of 4

Full-Text Articles in Life Sciences

Dietary Fat-Carbohydrate Combinations: Their Effects On Lipid Metabolism In Estrogen-Treated Rats, Beverly A. Clevidence Jan 1976

Dietary Fat-Carbohydrate Combinations: Their Effects On Lipid Metabolism In Estrogen-Treated Rats, Beverly A. Clevidence

Journal of the Arkansas Academy of Science

Female rats 4 weeks old were fed diets including beef tallow or safflower oil in combination with sucrose or rice starch. At 8 weeks of age, half the rats were orally administered 2 of estrogen (mestranol) in 5 μl of safflower oil and half were fed the vehicle only. After 10 to 14 days of estrogen treatment, rats were fasted and exsanguinated. Alterations were found in weight gain, liver weights, and levels of various lipids in plasma and liver. Most lipid levels were influenced by an interaction of mestranol with one of the dietary factors. No changes were observed in …


Effect Of Estrogen And/Or Supplemental Substrates On Uterine Utilization Of Pyruvate For Lipid Synthesis, Julie W. Harris Jan 1976

Effect Of Estrogen And/Or Supplemental Substrates On Uterine Utilization Of Pyruvate For Lipid Synthesis, Julie W. Harris

Journal of the Arkansas Academy of Science

Precursor incorporation into uterine lipids was examined in vitro after estradiol-17ß administration in immature female rats. The effect of adding supplemental substrates, glucose or gluconate, to the incubation medium on the labeling pattern of pyruvate-3-¹⁴C into uterine lipids was studied. Presence of supplemental substrate in the medium enhanced the incorporation of pyruvate into uterine lipids after two hours of in vivo estrogen treatment. It is suggested that estrogen's acceleration of pyruvate incorporation into lipid may be due to a concomitant effect on glucose metabolism.


Enzymes In Heloderma Horridum Venom, S. A. Murphy, Bob D. Johnson, D. H. Sifford Jan 1976

Enzymes In Heloderma Horridum Venom, S. A. Murphy, Bob D. Johnson, D. H. Sifford

Journal of the Arkansas Academy of Science

A mixture of venom and saliva from the lizard Heloderma horridum was analyzed for esterase, phosphomonoesterase, phosphodiesterase, 5'nucleotidase, and protease activities. Hydrolysis of N-benzoyl-L-arginine ethyl ester occurred at a pH optimum between pH 8.6 and 9.1 with a maximum activity of 452 units per mg per min. Hydrolysis of ptoluenesulfonyl-L-arginine methyl ester occurred at a pH optimum between pH 8.1 and 8.5 with a maximum of only 36 units per mg per min. One mg of the venom mixture liberated 9.3 μM of p nitrophenol from p-nitrophenyl phosphate per minute at an optimum pH between 8.2 and 8.3. Over a …


Influence Of Dietary Fats And Carbohydrates On Lipid Metabolism In Male And Female Rats, Marjorie Ellen Fitch Jan 1976

Influence Of Dietary Fats And Carbohydrates On Lipid Metabolism In Male And Female Rats, Marjorie Ellen Fitch

Journal of the Arkansas Academy of Science

Effects of dietary fats and carbohydrates on lipid metabolism in rats were studied. Male and female 4-week-old rats were divided into 8 groups and fed 4 fat-carbohydrate combinations (beef tallow or safflower oil, each with either sucrose or rice starch). After 4 weeks, animals were killed by exsanguination through the abdominal aorta and livers were removed. Plasma and liver cholesterol and phospholipids were determined qualitatively and quantitatively. Liver moisture, protein, and lipid and the fatty acid composition of the total liver lipid were determined quantitatively. Variations in growth, food efficiency, and lipid metabolism, particularly as manifested by the fatty acid …