Life Sciences Commons^™

Evaluating The Effect Of Manufacturing Porcine Epidemic Diarrhea Virus (Pedv)-Contaminated Feed On Subsequent Feed Mill Environmental Surface Contamination, L. L. Schumacher, R. A. Cochrane, C. E. Evans, J. R. Kalivoda, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, S. S. Dritz, Phillip Charles Gauger

Kansas Agricultural Experiment Station Research Reports

This study aimed to utilize the only known pilot feed mill facility approved for pathogenic feed agent use in the United States to evaluate the effect of manufacturing Porcine Epidemic Diarrhea Virus (PEDV)-contaminated feed on subsequent feed mill environmental surface contamination. In this study, PEDV inoculated feed was manufactured and conveyed on equipment along with four subsequent batches of PEDV-free feed. Equipment and environmental surfaces were sampled using swabs and analyzed for the presence of PEDV RNA by PCR. The experiment was replicated three times with decontamination of the feed mill and all equipment between replications. Overall, environmental swabs indicated …

Evaluating The Impact Of Maternal Vitamin D Supplementation On Sow Performance, Serum Vitamin Metabolites, Neonatal Muscle And Bone Characteristics, And Subsequent Pre-Weaning Pig Performance, J. R. Flohr, J. C. Woodworth, M. D. Tokach, S. S. Dritz, R. D. Goodband, J. M. Derouchey, J. R. Bergstrom

Kansas Agricultural Experiment Station Research Reports

A total of 56 gestating sows (PIC 1050; 35-d post-insemination) were used in a 30-d trial to determine the serum 25(OH)D₃response to increasing concentrations of vitamins D3. At initiation, sows were randomly allotted to 1 of 7 dietary vitamin D₃treatments (91, 363, 726, 1,451, 2,903, 5,806, or 11,612 IU of vitamin D₃/lb of complete diet) with 8 sows per treatment. All sows were fed 5.5 lb daily at 0800. Increasing vitamin D3 increased (quadratic; P<0.001) serum 25(OH)D₃with the response depicted by the following prediction equation:

Serum 25(OH)D3_,ng/mL = 35.1746 + (0.002353 × …

Determining The Minimum Infectious Dose Of Porcine Epidemic Diarrhea Virus (Pedv) In A Feed Matrix, L. L. Schumacher, J. C. Woodworth, C. R. Stark, C. K. Jones, R. A. Hesse, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach

Kansas Agricultural Experiment Station Research Reports

Understanding the magnitude of transmissible risk Porcine Epidemic Diarrhea Virus (PEDV)-infected feed imposes and establishing the minimum infectious dose of PEDV in a feed matrix are important components in strengthening virus prevention and control methods. In this study, an experiment was performed involving 30 crossbred, 10-d-old pigs that were used as a bioassay model for the minimum infectious dose of PEDV in feed. The PEDV was first diluted using tissue culture media to form 8 serial 10-fold dilutions. An aliquot of the original stock virus at 5.6 x 10⁵tissue culture infectious dose/ml (TCID₅₀/ml), each serial PEDV …

Effects Of Potential Detoxifying Agents On Growth Performance And Deoxynivalenol (Don) Urinary Balance Characteristics Of Nursery Pigs Fed Don-Contaminated Wheat, H. L. Frobose, E. W. Stephenson, M. D. Tokach, J. M. Derouchey, S. S. Dritz, R. D. Goodband, J. C. Woodworth, R. E. Musser

Kansas Agricultural Experiment Station Research Reports

Two experiments were conducted to evaluate the effects of detoxifying agents on the growth performance of nursery pigs fed diets contaminated with deoxynivalenol (DON). Naturally DON-contaminated wheat (6 ppm) replaced noncontaminated wheat in diets to achieve desired dietary DON concentrations. Basal ingredients were tested for mycotoxin and amino acid content prior to diet manufacturing. Diets were pelleted at 180˚F with a 45-s conditioning time.

A total of 238 barrows and gilts (PIC 327 × 1050; initially 29.6 ± 5.6 lb and 42 d of age) were used in a 21-d growth study. Pens of pigs were allotted by BW to …

Evaluating The Effects Of Maternal Vitamin D Supplementation On The Subsequent Growth Performance And Carcass Characteristics Of A Subsample Population Of Growing Pigs, J. R. Flohr, J. C. Woodworth, M. D. Tokach, S. S. Dritz, R. D. Goodband, J. M. Derouchey, J. R. Bergstrom

Kansas Agricultural Experiment Station Research Reports

A subsample of 448 growing pigs (PIC 327 × 1050), or approximately 50% of pigs weaned from sows fed varying dietary vitamin D regimens, were used in a split-plot design to determine the influence of maternal and nursery vitamin D regimens on growth performance. Sows were previously administered diets containing vitamin D as either: 1) low vitamin D₃(363 IU/lb); 2) medium vitamin D₃(907 IU/lb); 3) high vitamin D₃(4,354 IU/lb); or 4) 23μg 25(OH)D₃/lb (Hy-D, DSM Nutritional Products Inc, Parsippany, NJ) as described by Flohr et al. (20153) throughout gestation and …

Effects Of Amino Acid And Energy Intake During Late Gestation On Piglet Birth Weight And Reproductive Performance Of Gilts And Sows Housed Under Commercial Conditions, M. A. Goncalves, K. M. Gourley, S. S. Dritz, M. D. Tokach, N. M. Bello, J. M. Derouchey, J. C. Woodworth, R. D. Goodband

Kansas Agricultural Experiment Station Research Reports

The objective of this study was to determine the effects of amino acid (AA) and energy intake during late gestation on piglet birth weight and reproductive performance of high-performing gilts and sows housed under commercial conditions. At d 90 of gestation, a total of 1,102 females (PIC 1050) were housed in pens by parity group (P1 or P2+), blocked by weight within each pen, and each female was randomly assigned to dietary treatments within weight block. Dietary treatments consisted of combinations of 2 standardized ileal digestible (SID) AA (10.7 or 20.0 g SID Lys intake/d with other AA meeting or …

Evaluating The Efficacy Of A Novel Phytase Source, J. R. Flohr, J. M. Derouchey, M. D. Tokach, S. S. Dritz, J. C. Woodworth, R. D. Goodband

Kansas Agricultural Experiment Station Research Reports

A total of 350 nursery pigs (PIC 1050 barrows, initially 33.2 lb and 49 d of age) were used in a 21-d study to determine the aP release curve for a novel phytase product (Microtech 5,000, VTR Bio-tech Co., Guangdong, China). Pigs were randomly allotted to pens at arrival to the facility, and on d 0 of the trial pens were allotted to 1 of 7 treatments in a randomized complete block design. There were 5 pigs per pen and 10 pens per treatment. Pigs were fed corn-soybean meal-based diets formulated to 1.25% SID lysine. A single batch of the …

Effects Of Commercial Formaldehyde Inclusion And Lysine Level On Pig Performance Of 35- To 50-Lb Nursery Pigs, R. A. Cochrane, L. G. Sica, J. C. Woodworth, S. S. Dritz, C. R. Stark, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

A total of 299 pigs (DNA 400 × 200; initial BW 33.6 lb) were used in a 14-d study to determine the effects of two separate commercial formaldehyde products (Termin-8; Anitox Corp, Lawrenceville, GA and SalCURB; Kemin Industries, Inc., Des Moines, IA) on nursery pig performance. Dietary treatments were arranged in a 3 × 2 factorial design with three formaldehyde inclusions: none vs. 6.5 lb/ton SalCURB vs. 6.0 lb/ton Termin-8 and 2 Lys levels: Standard (1.25% SID Lys) vs. Low (1.10% SID Lys). Formaldehyde treatments were established based on supplier recommendations and diets were treated with supplier-specific equipment. Pens of …

Vitamin And Trace Minerals: A Survey Of Current Feeding Regimens, J. R. Flohr, M. D. Tokach, J. C. Woodworth, J. M. Derouchey, S. S. Dritz, R. D. Goodband

Kansas Agricultural Experiment Station Research Reports

Swine producers and nutritionists representing production systems across the United States were surveyed about added vitamin and trace mineral concentrations in swine diets used from March to August of 2014. Respondents were asked to provide the vitamin and trace mineral premix specifications and inclusion rates to calculate complete diet added vitamin and trace mineral concentrations. Weight ranges associated with feeding phases also were collected. In total, 18 production systems representing approximately 2.3 million sows (~40% of the U.S. sow herd) participated in the survey. Data were compiled into relatively consistent weight ranges across all participating producers. There were three nursery …

Determining The Impact Of By-O-Reg+ In Diets With Or Without A Feed Grade Antibiotic On Growth Performance Of Nursery Pigs, L. L. Thomas, J. C. Woodworth, R. D. Goodband, J. M. Derouchey, M. D. Tokach, S. S. Dritz

Kansas Agricultural Experiment Station Research Reports

A total of 717 nursery pigs (PIC C-29 × 28 and PIC L3-1050 × 1040, initially 12.48 ± 0.10 lb) from two consecutive nursery groups were used in a 35-d growth study. The objective was to determine the impact of increasing levels of By-O-Reg+ in diets with or without 50 g/ton Carbadox. By-O-Reg+ is a unique mixture of essential oils primarily based on oregano. It utilizes encapsulation technology to stabilize the essential oils and is suggested to have antimicrobial-like properties. The present study evaluated growth performance of nursery pigs fed 1 of 6 dietary treatments that were arranged as a …

Effect Of Thermal Mitigation On Porcine Epidemic Diarrhea Virus (Pedv)- Contaminated Feed, R. A. Cochrane, L. L. Schumacher, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rodger G. Main, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

Porcine Epidemic Diarrhea Virus (PEDV) is primarily transmitted by fecal-oral contamination. However, epidemiological evidence has shown that swine feed and ingredients may serve as potential vectors of transmission. Since it is known that PEDV is a heat-sensitive virus, we hypothesized that a conditioner and pellet mill mimicking commercial thermal processing would mitigate PEDV infectivity. To test this hypothesis, two experiments were designed to determine if different pellet mill conditioner retention times or temperatures would impact PEDV infectivity determined by polymerase chain reaction (PCR) analysis and bioassay. For the first study, a 3×3×2 factorial was utilized, with three pelleting temperatures (155, …

Utilizing Feed Sequencing To Decrease The Risk Of Porcine Epidemic Diarrhea Virus (Pedv) Cross-Contamination During Feed Manufacturing, L. L. Schumacher, R. A. Cochrane, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach

Kansas Agricultural Experiment Station Research Reports

Understanding key points of potential cross-contamination during the feed manufacturing process is important to developing efficacious methods to control or prevent transmission of pathogens into swine diets. In this study, an experiment was conducted involving 30 crossbred 10-d-old pigs that were used as a bioassay model for Porcine Epidemic Diarrhea Virus (PEDV) to determine the effects of feed batch sequencing on PEDV cross-contamination and subsequent infectivity. PEDV with a PCR cycle threshold value (Ct) of 11 was uniformly mixed into 4.5 kg of swine diet using a stainless steel bench top mixer validated for mixing efficiency. The inoculated feed was …

Evaluating Chemical Mitigation Of Porcine Epidemic Diarrhea Virus (Pedv) In Swine Feed And Ingredients, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, R. A. Hesse, Jianqiang Zhang, M. D. Tokach, J. Bai, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

Porcine Epidemic Diarrhea Virus (PEDV) is primarily transmitted by fecal-oral contamination. Research has confirmed swine feed or ingredients as potential vectors of transmission, so strategies are needed to mitigate PEDV in feed. The objective of this experiment was to evaluate the effectiveness of various chemical additives to prevent or mitigate post-processing PEDV contamination in swine feed and ingredients. Treatments were arranged in a 7 × 4 factorial with seven chemical treatments and four feed matrices. The chemical treatments included: negative control with no chemical addition, 0.3% commercial formaldehyde product, 1% sodium bisulfate, 1% sodium chlorate, 3% custom organic acid blend …

Evaluating Pellet And Meal Feeding Regimens On Finishing Pig Performance, Stomach Morphology, Carcass Characteristics, And Economics, J. A. De Jong, J. M. Derouchey, M. D. Tokach, S. S. Dritz, R. D. Goodband, M. Allerson

Kansas Agricultural Experiment Station Research Reports

A total of 2,100 pigs (PIC 327 × 1050, initially 68.8 lb) were used in a 118-d trial to determine the effects of pellet feeding regimens on finishing pig growth performance, stomach morphology, and carcass characteristics. Pens of pigs were balanced by initial BW and randomly allotted to 1 of 6 dietary treatments (14 pens/treatment with 25 pigs/pen). Pens were sorted by gender allowing for 7 barrow and 7 gilt pens/treatment. The same corn-soybean meal–based diets containing 15% dried distillers grains with solubles were used for all treatments and fed in 5 phases. The 6 treatments included a meal or …

Effect Of Diet Type And Added Copper On Growth Performance, Carcass Characteristics, Energy Digestibility, Gut Morphology, And Mucosal Mrna Expression Of Finishing Pigs, K. F. Coble, D. D. Burnett, R. D. Goodband, J. M. Gonzales, J. Usry, M. D. Tokach, J. R. Pluske, J. M. Derouchey, J. C. Woodworth, S. S. Dritz, J. R. Flohr, M. A. Vaughn

Kansas Agricultural Experiment Station Research Reports

A total of 757 pigs (PIC 337 × 1050, initially 60.8 lb) were used to determine the effects of added Cu (TBCC, tribasic copper chloride, IntelliBond C; Micronutrients, Inc., Indianapolis, IN) and diet type on growth performance, carcass characteristics, energy digestibility, gut morphology, and mucosal mRNA expression of finishing pigs. Pens of pigs were allotted to 1 of 4 dietary treatments, balanced on average pen weight in a randomized complete-block design with 26 to 28 pigs per pen and 7 replications per treatment. Treatments were arranged as a 2 × 2 factorial with main effects of diet type, a corn-soybean …