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Full-Text Articles in Life Sciences
Does The Embedding Chemistry Interact With Tissue?, B. E. Causton
Does The Embedding Chemistry Interact With Tissue?, B. E. Causton
Scanning Electron Microscopy
Resins and resin compounds interact with tissue in two ways, physico-chemically and by chemical reaction. The physico-chemical influences affect both the structure and biological activity of the tissue causing proteins to change shape or phase equilibria to be disrupted by a change in solvent or by the growth of polymer networks within existing biological polymeric structures. Chemical reactivity between tissue and resin components also reduces biological activity by changing either the structure of polymeric matter already present, e.g., crosslinking proteins, grafting hydrophobic resins onto hydrophilic protein backbones, or by modifying the hydrophobicity of specific sites, e.g., acylation of amino groups …
The Morphology Of Thin Metal Coatings Formed By Rotary Shadowing Biological Species In High Vacuum, J. A. Panitz
The Morphology Of Thin Metal Coatings Formed By Rotary Shadowing Biological Species In High Vacuum, J. A. Panitz
Scanning Electron Microscopy
Rotary Shadowing has been used to increase the image contrast of biological species observed during edge-projection imaging in the transmission electron microscope. In this imaging mode, biological species are adsorbed from aqueous solutions onto a highly curved substrate and viewed, over its edge, in a direction parallel to its surface. Since the substrate is not placed between a biological adsorbate and the photographic emulsion that records its image, any material can be used as a support (including high-Z metals and semiconductors). Binding to these technologically interesting materials is observed with unusual clarity and contrast, even at 200kV. Individual adsorbates and …