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Interactive Effects Of Anthropogenic, Environmental, And Biotic Stressors On Multiple Endpoints In Hyla Chrysoscelis, Carol A. Britson, Stephen T. Threlkeld

Journal of the Iowa Academy of Science: JIAS

Multiple stressors have been proposed as causative agents for declining populations and increased incidence of malformations in amphibians although few studies have examined possible interactions among these stressors. We measured interactive effects of UV radiation, three chemicals, and interspecific competition (with Rana 1phenocephala) on multiple endpoints in Hyla chrysoscelis using a center point- and chemical-free control-enhanced 2⁴ factorial design. UV radiation was transmitted or filtered using OP-4 or OP-3 acrylite filters installed above 72, 500-liter mesocosms on 16 May 1997. Methyl mercury, chlorpyrifos, and atrazine were applied at levels of 0, 10, 50, and 100 % of 400 ppb, …