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Life Sciences Commons

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Journal

Minnesota State University, Mankato

Genes

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Full-Text Articles in Life Sciences

Identification Of Snps In The Coding Region Of Human Mtdna, Cassidy Punt, Elizabeth Smalley Aug 2014

Identification Of Snps In The Coding Region Of Human Mtdna, Cassidy Punt, Elizabeth Smalley

Journal of Undergraduate Research at Minnesota State University, Mankato

Two novel single nucleotide polymorphisms (SNPs) were discovered within the coding region of the NADH dehydrogenase subunit 2 gene of mitochondrial DNA (mtDNA). mtDNA is of particular importance in forensic analysis as well as in the study of the origin and dispersal of humans. Two segments of the coding region of human mtDNA, as well as the hyper-variable region 2 (HV 2) were selected and sequenced in order to determine if any previously unknown SNPs were present in our test subjects. Target regions were designed to include known SNPs; appropriate primers were developed using the OLIGO 6 Primer Analysis Software. …


Biofilm Formation By Escherichia Coli Csga And Fima Mutants, Nicole Snyder, Sean Willaert Aug 2014

Biofilm Formation By Escherichia Coli Csga And Fima Mutants, Nicole Snyder, Sean Willaert

Journal of Undergraduate Research at Minnesota State University, Mankato

Biofilms are a structured community of bacterial cells enclosed in a self-produced polymeric matrix and adherent to an inert or living surface. These structures and the organisms that cause them can pose a very serious problem if they colonize on medical devices. This is because biofilms have the ability to communicate within the colony and with other organisms that might attach to the surface, acting like a community working together. Biofilms allow the organism to be resistant to harsh and unfavorable conditions allowing them to survive longer and spread. Several genes in Escherichia coli (E. coli) have been associated …