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A Nonhomologous End-Joining Mutant For Neurospora Sitophila Research, Nicholas A. Rhoades, Elise K. Webber, Thomas M. Hammond
A Nonhomologous End-Joining Mutant For Neurospora Sitophila Research, Nicholas A. Rhoades, Elise K. Webber, Thomas M. Hammond
Fungal Genetics Reports
Disruption of the nonhomologous end-joining (NHEJ) pathway has been shown to increase the efficiency of transgene integration into targeted genomic locations of Neurospora crassa and other fungi. Here, we report that a similar phenomenon occurs in a second Neurospora species: N. sitophila. Specifically, we show that deletion of N. sitophila mus-51 increases the efficiency of targeted-transgene integration, presumably by disrupting NHEJ. Researchers interested in obtaining the N. sitophila mus-51∆ strains described in this study can obtain them from the Fungal Genetics Stock Center (FGSC, Kansas State University, Manhattan, KS).