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Full-Text Articles in Life Sciences
Chemical, Enzymatic, And Antioxidant Enrichments Of Full-Fat Soybean And Sunflower Meal By Bacillus Subtilis (Atcc$^{®}$ 6633$^{Tm}$) Fermentation Using A Solid-State Bioreactor, Yaşar Karakurt, Damla Güverci̇n, Sercan Önder, Ci̇van Çeli̇k, Ramazan Tosun, Burak Baran, Sulhatti̇n Yaşar
Chemical, Enzymatic, And Antioxidant Enrichments Of Full-Fat Soybean And Sunflower Meal By Bacillus Subtilis (Atcc$^{®}$ 6633$^{Tm}$) Fermentation Using A Solid-State Bioreactor, Yaşar Karakurt, Damla Güverci̇n, Sercan Önder, Ci̇van Çeli̇k, Ramazan Tosun, Burak Baran, Sulhatti̇n Yaşar
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to fortify nutritional and chemical composition of sunflower meal (SFM) and full-fat soybean (FFSB) used as a protein source in farm animal nutrition by Bacillus subtilis ATCC 6633 fermentation. An optimized set of fermentation parameters (60% moisture, 6.5-7.0 pH, 30-35 °C temperature, continuous aeration of 0.5-1.0 L/min and agitation of 20-100 rpm) were used to ferment SFM and FFSB samples with or without using bacterial inoculant for 0, 24, 48, and 72 h. The results indicated that the total organic acids (TOA) contents and crude ash (CA) of SFM and FFSB increased significantly, but there were …
Effect Of Calsporin On Nursing Piglet Growth Performance And Fecal Microflora, M. B. Menegat, C. M. Vier, H. S. Cemin, D. Shawk, J. M. Derouchey, J. C. Woodworth, M. D. Tokach, S. S. Dritz, R. D. Goodband
Effect Of Calsporin On Nursing Piglet Growth Performance And Fecal Microflora, M. B. Menegat, C. M. Vier, H. S. Cemin, D. Shawk, J. M. Derouchey, J. C. Woodworth, M. D. Tokach, S. S. Dritz, R. D. Goodband
Kansas Agricultural Experiment Station Research Reports
A total of 26 lactating sows (DNA 241, DNA Genetics, Columbus, NE) and litters were used in a discovery study to evaluate the effects of Calsporin, a direct-fed microbial containingBacillus subtilisC-3102, on fecal microflora of nursing pigs. The treatments consisted of providing a daily oral dose of Calsporin or a placebo control to piglets during the nursing phase. Sows were randomly assigned to treatments based on farrowing date, parity, and initial BW. The treatments were applied individually to piglets once a day from d 2 after farrowing and equalization until weaning on d 19. Sow BW, sow ADFI, …
Cloning And Expression Of The Gene Encoding Industrial \Alpha-Amylase From Bacillus Subtilis Orba97 In Escherichia Coli And Bacillus Subtilis Yb886, Numan Özcan
Turkish Journal of Veterinary & Animal Sciences
The \alpha-amylase encoding gene from Bacillus subtilis ORBA97 strain was cloned and expressed in Escherichia coli with the plasmid vector called pUC18 as well as in the \alpha-amylase negative Bacillus subtilis YB886 strain by using the pUB110 vector. The specific activity of the enzyme was determined to be 5.052 µmol (mg protein min)^{-1}. Zymogram analysis revealed no sign of protease degradation in the YB886 strain. The optimum temperature and pH of the enzyme were found to be 50°C and 5 respectively. Relative enzyme activity decreased to 36% of the original activity when it was subjected to a temperature of 90°C …
Molecular Cloning Of An \Alpha-Amylase Gene From Bacillus Subtilis Rskk246 And Its Expression In Escherichia Coli And In Bacillus Subtilis, Numan Özcan, Adem Altinalan, Mehmet Sai̇t Eki̇nci̇
Molecular Cloning Of An \Alpha-Amylase Gene From Bacillus Subtilis Rskk246 And Its Expression In Escherichia Coli And In Bacillus Subtilis, Numan Özcan, Adem Altinalan, Mehmet Sai̇t Eki̇nci̇
Turkish Journal of Veterinary & Animal Sciences
Bacillus subtilis RSKK246 was found to produce approximately a 65-kDa \alpha-amylase enzyme. A gene was isolated encoding \alpha-amylase activity that corresponded to this size and was inserted into pUC18 plasmid which was transferred to Escherichia coli. An approximately 1.7kbp fragment, which contains a whole \alpha-amylase gene, was excised and inserted into pUB110 and then transferred into the different B. subtilis strains including RSKK246, RSKK243, RSKK244, YB886 and ORBAM. The a-amylase gene was cloned into the plasmids and expressed with its own promoter, and this promoter sequence seemed to function in the E. coli and in all B. subtilis strains. Specific …
Cloning Of Bacillus Subtilis Rskk243 Bifunctional Xylanase Gene In E. Coli And B. Subtilis And Enzyme Characterization, Bahri̇ Devri̇m Özcan, Numan Özcan
Cloning Of Bacillus Subtilis Rskk243 Bifunctional Xylanase Gene In E. Coli And B. Subtilis And Enzyme Characterization, Bahri̇ Devri̇m Özcan, Numan Özcan
Turkish Journal of Veterinary & Animal Sciences
A bifunctional gene xylanase of B. subtilis RSKK243 encoding detectable beta(1,4) glucanase (CMCase) activity in addition to a high level of main xylanase activity was cloned and expressed in Escherichia coli with pUC18 plasmid and in various Bacillus subtilis strains with pUB110 vector. For enzymatic analysis and plasmid isolation, the gene was also expressed in xylanase and beta glucanase negative B.subtilis YB886 strain with pUB110 vector. The pUB110 with bifunctional xylanase gene was transferred into B. subtilis RSKK243 for maximizing the enzyme yield, as well as into other RSKK strains to create novel strains with multiple enzyme capacities. Recombinant strains …