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Full-Text Articles in Life Sciences
Pluronic Pf68 Increases Transfection Efficiency In Electroporationof Mesenchymal Stem Cells, Safa Aydin, Mehmet Emi̇r Yalvaç, Ferruh Özcan, Fi̇kretti̇n Şahi̇n
Pluronic Pf68 Increases Transfection Efficiency In Electroporationof Mesenchymal Stem Cells, Safa Aydin, Mehmet Emi̇r Yalvaç, Ferruh Özcan, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
Electroporation is a safe and efficient method to transfect human tooth germ stem cells (hTGSCs), which are mainly mesenchymal stem cells (MSCs). In this study, pluronic F68 (PF68), a nonionic and low-foaming surfactant, was used for the first time to increase the transfection efficiency in electroporation of hTGSCs. Our results revealed that when PF68 was used in the preincubation, electroporation, and recovery medium, it dramatically increased transfection efficiency. Moreover, use of PF68 did not alter the differentiation capacity of hTGSCs, whereas cells electroporated without PF68 had significantly lower expression of Oct-4, pluripotency marker gene, and alkaline phosphatase (ALP) activity upon …
Cardiomyogenic Differentiation Potential Of Human Lipoaspirate-Derivedstem Cells On Hyaluronic Acid/Gelatin Plasma Gels, Esra Göv, Hali̇me Kenar, Zehra Seda Halbutoğullari, Kazim Yalçin Arğa, Erdal Karaöz
Cardiomyogenic Differentiation Potential Of Human Lipoaspirate-Derivedstem Cells On Hyaluronic Acid/Gelatin Plasma Gels, Esra Göv, Hali̇me Kenar, Zehra Seda Halbutoğullari, Kazim Yalçin Arğa, Erdal Karaöz
Turkish Journal of Biology
Cardiomyogenic differentiation from mesenchymal stem cells has emerged as a novel approach for repair of damaged myocardium. Cell transplantation through direct cell injection is not an optimal method due to the lack of cell-extracellular matrix interactions. In the present study, differentiation potential of human adipose-derived stem cells (ASCs) to cardiomyocytes has been investigated by growing them on hyaluronic acid/gelatin (HA/G) plasma gels and coverslips and supplementing the growth medium with chemical modifiers (activin-a, BMP-4, insulin, valproic acid, and 5-azacytidine) in various combinations. The HA/G plasma gels were produced from human blood plasma-derived fibrinogen, gelatin, and human umbilical cord-derived hyaluronic acid. …
Myelin Disorders And Stem Cells: As Therapies And Models, Fatmagül İlayda Aydinli, Eşref Çeli̇k, Burcu Kurt Vatandaşlar, Bi̇lal Ersen Kerman
Myelin Disorders And Stem Cells: As Therapies And Models, Fatmagül İlayda Aydinli, Eşref Çeli̇k, Burcu Kurt Vatandaşlar, Bi̇lal Ersen Kerman
Turkish Journal of Biology
Myelin disorders burden millions of people around the world, yet existing therapies are inadequate to cure them. Current remedies commonly treat the symptoms with minimal to no effect on the actual cause of the disorder. The basis and/or the mechanism of demyelination is not known for many of the disorders either. In recent years, stem cells of variable origin have been used in clinical trials as transplant agents to restore the defective biochemical process or the damaged tissue. We summarize the outcomes of these trials for demyelination disorders. The capability of reprograming mature cells into stem cells equips researchers with …
Current Status Of Stem Cell Therapy: Opportunities And Limitations, Elena Rusu, Laura Georgiana Necula, Ana Iulia Neagu, Mihail Alecu, Cristian Stan, Radu Albulescu, Cristiana Pistol Tanase
Current Status Of Stem Cell Therapy: Opportunities And Limitations, Elena Rusu, Laura Georgiana Necula, Ana Iulia Neagu, Mihail Alecu, Cristian Stan, Radu Albulescu, Cristiana Pistol Tanase
Turkish Journal of Biology
Over recent years stem cells have stood out as a promising tool for regenerative medicine, providing alternative therapeutic solutions for a large number of diseases. Many clinical trials using stem cells or induced pluripotent stem cells are focused on the repair and regeneration of various tissues and organs in degenerative diseases, whose current treatment only succeeds in slowing down the progression of the disease. Although the preliminary results are interesting, further studies are required in order to evaluate the safety and benefits of stem cell therapy, considering the teratoma development and ethical considerations in embryonic stem cell cases or reprogramming-induced …
Human Adipose-Derived Stem Cells Differentiation Into Epidermal Cells And Interaction With Human Keratinocytes In Coculture, Alexandra Gaspar, Daniel Constantin, Ana-Maria Seciu, Lucia Moldovan, Oana Craciunescu, Elena Ganea
Human Adipose-Derived Stem Cells Differentiation Into Epidermal Cells And Interaction With Human Keratinocytes In Coculture, Alexandra Gaspar, Daniel Constantin, Ana-Maria Seciu, Lucia Moldovan, Oana Craciunescu, Elena Ganea
Turkish Journal of Biology
Current procedures used for skin injury reepithelization have yet to improve. At present, stem cell-based therapies provide great potential in repairing different damaged tissues due to the multipotent character of these cells. This study aimed to investigate the in vitro differentiation capacity of human adipose-derived stem cells (ASCs) into epidermal cells and to evaluate their interaction with HaCaT keratinocytes in coculture experimental models that mimicked the living environment of human skin. The mesenchymal phenotype of isolated stem cells was indicated by their spindle-shaped morphology and the expression of specific surface markers CD73, CD90, and CD105. After 21 days of cultivation …