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Agrobacterium-Mediated Transformation Of Five Inbred Maize Lines With The Brittle 2 Gene, Thuy Ninh Nguyen, Thi Luong Tran, Duc Thanh Nguyen
Agrobacterium-Mediated Transformation Of Five Inbred Maize Lines With The Brittle 2 Gene, Thuy Ninh Nguyen, Thi Luong Tran, Duc Thanh Nguyen
Turkish Journal of Biology
The Brittle 2 (Bt2) gene encodes a small subunit of ADP-glucose pyrophosphorylase (AGPase) enzyme, one of the enzymes that play an important role in endosperm starch biosynthesis. We carried out a study to transfer the Bt2 gene into several inbred maize lines and regenerate transgenic maize plants possessing the transgene, which can be used to further study the role of the Bt2 gene in starch biosynthesis. Agrobacterium tumefaciens strains C58 and EHA105 harboring the transformation vector pCambia2300/Ubi-Bt2-Nos were used to transfer the Bt2 gene into five inbred maize lines, H14, H26, H240, H95, and N18. Transformed calli were selected on …
Direct Shoot Organogenesis And Agrobacterium Tumefaciens Mediated Transformation Of Solanum Trilobatum L., Jayabalan Shilpha, Manoharan Jayashre, Muthiah Joe Virgin Largia, Manikandan Ramesh
Direct Shoot Organogenesis And Agrobacterium Tumefaciens Mediated Transformation Of Solanum Trilobatum L., Jayabalan Shilpha, Manoharan Jayashre, Muthiah Joe Virgin Largia, Manikandan Ramesh
Turkish Journal of Biology
Solanum trilobatum L. İs one of the most valued medicinal plants of Indian Ayurveda and Siddha medicine. An efficient protocol for direct shoot organogenesis and Agrobacterium tumefaciens mediated transformation using in vitro derived leaf explants of S. Trilobatum was established for the first time. High frequency direct shoot regeneration (89%) was achieved in MS medium containing 2 mg/L 6-benzylaminopurine and 0.25 mg/L indole-3-acetic acid. The Agrobacterium strain EHA 105 harboring the binary vector pCAMBIA 1301, which contained hygromycin phosphotransferase (hpt) as a selectable marker gene and β-glucuronidase (gus) as a reporter gene, was used for the transformation. The factors that …