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First Record Of A Npv Isolated From Spodoptera Littoralis (Boisd.) (Lepidoptera: Noctuidae) In Turkey And Its Molecular Identification According To The Partial Lef-8 Gene, Umut Toprak, M. Oktay Gürkan
First Record Of A Npv Isolated From Spodoptera Littoralis (Boisd.) (Lepidoptera: Noctuidae) In Turkey And Its Molecular Identification According To The Partial Lef-8 Gene, Umut Toprak, M. Oktay Gürkan
Turkish Journal of Biology
Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is an important pest in Turkey and causes extensive economic losses in many cultivated crops. The viruses within the family Baculoviridae are specific pathogens of insects. The nucleopolyhedroviruses (NPVs), 1 of the 2 genera of this family, have been isolated from many insect orders, primarily from the lepidopterans. In our study, a local NPV was isolated from the larvae of Spodoptera littoralis fed in the cotton fields in southern Turkey (Mersin). For the molecular identification of the virus, polyhedra (occlusion bodies [OBs]) were isolated from insects and total DNA extraction was performed using a commercial …